Carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds for bone tissue engineering applications

Detalhes bibliográficos
Autor(a) principal: Lemos, R.
Data de Publicação: 2021
Outros Autores: Maia, F. Raquel, Ribeiro, Viviana Pinto, Costa, João Pedro Bebiano, Coutinho, Paulo J. G., Reis, R. L., Oliveira, J. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/74568
Resumo: Accepted Manuscript
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spelling Carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds for bone tissue engineering applicationsBone Tissue EngineeringCarbon nanotubesDecellularized cell-derived matrixHierarchical scaffoldsSilk fibroinCiências Médicas::Biotecnologia MédicaEngenharia e Tecnologia::Engenharia dos MateriaisEngenharia e Tecnologia::Engenharia MédicaScience & TechnologyAccepted ManuscriptIn bone tissue engineering, the development of advanced biomimetic scaffolds has led to the quest for biomotifs in scaffold design that better recreate bone matrix structure and composition and hierarchy at different length scales. In this study, an advanced hierarchical scaffold consisting of silk fibroin combined with decellularized cell-derived extracellular matrix and reinforced with carbon nanotubes was developed. The goal of the carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds is to harvest the individual properties of its constituents to introduce hierarchical capacity in order to improve standard silk fibroin scaffolds. The scaffolds were fabricated using enzymatic cross-linking, freeze modeling, and decellularization methods. The developed scaffolds were assessed for pore structure and mechanical properties showing satisfying results to be used in bone regeneration. The developed carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds showed to be bioactive in vitro and expressed no hemolytic effect. Furthermore, cellular in vitro studies on human adipose-derived stem cells (hASCs) showed that scaffolds supported cell proliferation. The hASCs seeded onto these scaffolds evidenced similar metabolic activity to standard silk fibroin scaffolds but increased ALP activity. The histological stainings showed cells infiltration into the scaffolds and visible collagen production. The expression of several osteogenic markers was investigated, further supporting the osteogenic potential of the developed carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds. The hemolytic assay demonstrated the hemocompatibility of the hierarchical scaffolds. Overall, the carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds presented the required architecture for bone tissue engineering applications.Research and Innovation Staff Exchanges (RISE) action (H2020 Marie Skłodowska-Curie actions) for funds obtained through the BAMOS project (H2020-MSCA-RISE-2016-73415) and the R&D Project KOAT PTDC/BTMMAT/29760/2017 (POCI-01-0145- FEDER-029760), financed by Fundação para a Ciência e a Tecnologia (FCT) and co-financed by FEDER and POCI. F.R.M. acknowledges FCT for her contract under the Transitional Rule DL 57/2016 (CTTI-57/18-I3BS(5)). V.P.R. acknowledges the Junior Researcher contracts (POCI-01-0145-FEDER-031367; POCI-01-0145-FEDER-029139) attributed by the FCT under the projects Fun4TE project (PTDC/EMD-EMD/31367/2017) and BLiver (PTDC/EMD-EMD/29139/2017). J.B.C. acknowledges the Junior Researcher contract (POCI-01-0145-FEDER-031367) attributed by FCT to the Fun4TE project (PTDC/EMDEMD/31367/2017).Royal Society of ChemistryUniversidade do MinhoLemos, R.Maia, F. RaquelRibeiro, Viviana PintoCosta, João Pedro BebianoCoutinho, Paulo J. G.Reis, R. L.Oliveira, J. M.2021-102021-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/74568engLemos R., Maia F. R., Ribeiro V. P., Costa J. B., Coutinho P., Reis R. L., Oliveira J. M. Carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds for bone tissue engineering applications, Journal Of Materials Chemistry B, doi:10.1039/D1TB01972D, 20212050-750X10.1039/D1TB01972D34761792https://pubs.rsc.org/en/content/articlehtml/2021/tb/d1tb01972dinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:37:34Zoai:repositorium.sdum.uminho.pt:1822/74568Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:33:52.614707Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds for bone tissue engineering applications
title Carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds for bone tissue engineering applications
spellingShingle Carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds for bone tissue engineering applications
Lemos, R.
Bone Tissue Engineering
Carbon nanotubes
Decellularized cell-derived matrix
Hierarchical scaffolds
Silk fibroin
Ciências Médicas::Biotecnologia Médica
Engenharia e Tecnologia::Engenharia dos Materiais
Engenharia e Tecnologia::Engenharia Médica
Science & Technology
title_short Carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds for bone tissue engineering applications
title_full Carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds for bone tissue engineering applications
title_fullStr Carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds for bone tissue engineering applications
title_full_unstemmed Carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds for bone tissue engineering applications
title_sort Carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds for bone tissue engineering applications
author Lemos, R.
author_facet Lemos, R.
Maia, F. Raquel
Ribeiro, Viviana Pinto
Costa, João Pedro Bebiano
Coutinho, Paulo J. G.
Reis, R. L.
Oliveira, J. M.
author_role author
author2 Maia, F. Raquel
Ribeiro, Viviana Pinto
Costa, João Pedro Bebiano
Coutinho, Paulo J. G.
Reis, R. L.
Oliveira, J. M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Lemos, R.
Maia, F. Raquel
Ribeiro, Viviana Pinto
Costa, João Pedro Bebiano
Coutinho, Paulo J. G.
Reis, R. L.
Oliveira, J. M.
dc.subject.por.fl_str_mv Bone Tissue Engineering
Carbon nanotubes
Decellularized cell-derived matrix
Hierarchical scaffolds
Silk fibroin
Ciências Médicas::Biotecnologia Médica
Engenharia e Tecnologia::Engenharia dos Materiais
Engenharia e Tecnologia::Engenharia Médica
Science & Technology
topic Bone Tissue Engineering
Carbon nanotubes
Decellularized cell-derived matrix
Hierarchical scaffolds
Silk fibroin
Ciências Médicas::Biotecnologia Médica
Engenharia e Tecnologia::Engenharia dos Materiais
Engenharia e Tecnologia::Engenharia Médica
Science & Technology
description Accepted Manuscript
publishDate 2021
dc.date.none.fl_str_mv 2021-10
2021-10-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/74568
url https://hdl.handle.net/1822/74568
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lemos R., Maia F. R., Ribeiro V. P., Costa J. B., Coutinho P., Reis R. L., Oliveira J. M. Carbon nanotubes-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds for bone tissue engineering applications, Journal Of Materials Chemistry B, doi:10.1039/D1TB01972D, 2021
2050-750X
10.1039/D1TB01972D
34761792
https://pubs.rsc.org/en/content/articlehtml/2021/tb/d1tb01972d
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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