Magnetic bioreactor for magneto-, mechano- and electroactive tissue engineering strategies
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/65638 |
Resumo: | Biomimetic bioreactor systems are increasingly being developed for tissue engineering applications, due to their ability to recreate the native cell/tissue microenvironment. Regarding bone-related diseases and considering the piezoelectric nature of bone, piezoelectric scaffolds electromechanically stimulated by a bioreactor, providing the stimuli to the cells, allows a biomimetic approach and thus, mimicking the required microenvironment for effective growth and differentiation of bone cells. In this work, a bioreactor has been designed and built allowing to magnetically stimulate magnetoelectric scaffolds and therefore provide mechanical and electrical stimuli to the cells through magnetomechanical or magnetoelectrical effects, depending on the piezoelectric nature of the scaffold. While mechanical bioreactors need direct application of the stimuli on the scaffolds, the herein proposed magnetic bioreactors allow for a remote stimulation without direct contact with the material. Thus, the stimuli application (23 mT at a frequency of 0.3 Hz) to cells seeded on the magnetoelectric, leads to an increase in cell viability of almost 30% with respect to cell culture under static conditions. This could be valuable to mimic what occurs in the human body and for application in immobilized patients. Thus, special emphasis has been placed on the control, design and modeling parameters governing the bioreactor as well as its functional mechanism. |
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Magnetic bioreactor for magneto-, mechano- and electroactive tissue engineering strategiesmagnetic bioreactormagnetoactive scaffoldstissue engineeringmagnetic actuatormagnetoelectric stimulationScience & TechnologyBiomimetic bioreactor systems are increasingly being developed for tissue engineering applications, due to their ability to recreate the native cell/tissue microenvironment. Regarding bone-related diseases and considering the piezoelectric nature of bone, piezoelectric scaffolds electromechanically stimulated by a bioreactor, providing the stimuli to the cells, allows a biomimetic approach and thus, mimicking the required microenvironment for effective growth and differentiation of bone cells. In this work, a bioreactor has been designed and built allowing to magnetically stimulate magnetoelectric scaffolds and therefore provide mechanical and electrical stimuli to the cells through magnetomechanical or magnetoelectrical effects, depending on the piezoelectric nature of the scaffold. While mechanical bioreactors need direct application of the stimuli on the scaffolds, the herein proposed magnetic bioreactors allow for a remote stimulation without direct contact with the material. Thus, the stimuli application (23 mT at a frequency of 0.3 Hz) to cells seeded on the magnetoelectric, leads to an increase in cell viability of almost 30% with respect to cell culture under static conditions. This could be valuable to mimic what occurs in the human body and for application in immobilized patients. Thus, special emphasis has been placed on the control, design and modeling parameters governing the bioreactor as well as its functional mechanism.FCT—Fundação para a Ciência e Tecnologia: UID/FIS/04650/2020; PTDC/BTM-MAT/28237/2017; PTDC/EMD-EMD/28159/2017 and SFRH/BPD/121464/2016. Spanish Ministry of Economy and Competitiveness (MINECO): MAT2016–76039-C4–3-R (AEI/FEDER, UE). Basque Government Industry and Education Department: ELKARTEK, PIB and PIBA (PIBA−2018–06) programs, respectively.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoCastro, NelsonFernandes, MargaridaRibeiro, ClarisseCorreia, VitorMinguez, RikardoLanceros-Méndez, S.2020-06-122020-06-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/65638engCastro, Nelson; Fernandes, Margarida; Ribeiro, Clarisse; Correia, Vítor; Minguez, Rikardo; Lanceros-Méndez, Senentxu, Magnetic bioreactor for magneto-, mechano- and electroactive tissue engineering strategies. Sensors, 20(12), 3340, 20201424-82201424-321010.3390/s2012334032545551http://www.mdpi.com/journal/sensorsinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:47:04Zoai:repositorium.sdum.uminho.pt:1822/65638Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:45:08.122511Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Magnetic bioreactor for magneto-, mechano- and electroactive tissue engineering strategies |
title |
Magnetic bioreactor for magneto-, mechano- and electroactive tissue engineering strategies |
spellingShingle |
Magnetic bioreactor for magneto-, mechano- and electroactive tissue engineering strategies Castro, Nelson magnetic bioreactor magnetoactive scaffolds tissue engineering magnetic actuator magnetoelectric stimulation Science & Technology |
title_short |
Magnetic bioreactor for magneto-, mechano- and electroactive tissue engineering strategies |
title_full |
Magnetic bioreactor for magneto-, mechano- and electroactive tissue engineering strategies |
title_fullStr |
Magnetic bioreactor for magneto-, mechano- and electroactive tissue engineering strategies |
title_full_unstemmed |
Magnetic bioreactor for magneto-, mechano- and electroactive tissue engineering strategies |
title_sort |
Magnetic bioreactor for magneto-, mechano- and electroactive tissue engineering strategies |
author |
Castro, Nelson |
author_facet |
Castro, Nelson Fernandes, Margarida Ribeiro, Clarisse Correia, Vitor Minguez, Rikardo Lanceros-Méndez, S. |
author_role |
author |
author2 |
Fernandes, Margarida Ribeiro, Clarisse Correia, Vitor Minguez, Rikardo Lanceros-Méndez, S. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Castro, Nelson Fernandes, Margarida Ribeiro, Clarisse Correia, Vitor Minguez, Rikardo Lanceros-Méndez, S. |
dc.subject.por.fl_str_mv |
magnetic bioreactor magnetoactive scaffolds tissue engineering magnetic actuator magnetoelectric stimulation Science & Technology |
topic |
magnetic bioreactor magnetoactive scaffolds tissue engineering magnetic actuator magnetoelectric stimulation Science & Technology |
description |
Biomimetic bioreactor systems are increasingly being developed for tissue engineering applications, due to their ability to recreate the native cell/tissue microenvironment. Regarding bone-related diseases and considering the piezoelectric nature of bone, piezoelectric scaffolds electromechanically stimulated by a bioreactor, providing the stimuli to the cells, allows a biomimetic approach and thus, mimicking the required microenvironment for effective growth and differentiation of bone cells. In this work, a bioreactor has been designed and built allowing to magnetically stimulate magnetoelectric scaffolds and therefore provide mechanical and electrical stimuli to the cells through magnetomechanical or magnetoelectrical effects, depending on the piezoelectric nature of the scaffold. While mechanical bioreactors need direct application of the stimuli on the scaffolds, the herein proposed magnetic bioreactors allow for a remote stimulation without direct contact with the material. Thus, the stimuli application (23 mT at a frequency of 0.3 Hz) to cells seeded on the magnetoelectric, leads to an increase in cell viability of almost 30% with respect to cell culture under static conditions. This could be valuable to mimic what occurs in the human body and for application in immobilized patients. Thus, special emphasis has been placed on the control, design and modeling parameters governing the bioreactor as well as its functional mechanism. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-12 2020-06-12T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/65638 |
url |
http://hdl.handle.net/1822/65638 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Castro, Nelson; Fernandes, Margarida; Ribeiro, Clarisse; Correia, Vítor; Minguez, Rikardo; Lanceros-Méndez, Senentxu, Magnetic bioreactor for magneto-, mechano- and electroactive tissue engineering strategies. Sensors, 20(12), 3340, 2020 1424-8220 1424-3210 10.3390/s20123340 32545551 http://www.mdpi.com/journal/sensors |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133015203381248 |