Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA

Detalhes bibliográficos
Autor(a) principal: Crnolatac, Ivo
Data de Publicação: 2020
Outros Autores: Giestas, Letícia, Horvat, Gordan, Parola, António Jorge, Piantanida, Ivo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/114694
Resumo: UIDB/50006/2020 ROTEIRO/0031/2013-PINFRA/22161/2016 HrZZ IP-2018-01-5475
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spelling Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNAChalconesDNA/RNA bindingFlavylium cationFluorescence, circular dichroismpH controlAnalytical ChemistryPhysical and Theoretical ChemistryUIDB/50006/2020 ROTEIRO/0031/2013-PINFRA/22161/2016 HrZZ IP-2018-01-5475The interaction of 4′-(N,N-dimethylamino)-6-hydroxyflavylium cation with double stranded (ds-) DNA/RNA was studied by UV/Vis spectrophotometry, circular dichroism (CD), and also steady-state and time-resolved emission spectroscopies at neutral and weakly acidic conditions. At pH 5, the studied molecule, in its flavylium cationic form, showed considerable binding affinities (5 < logKs < 6) for all ds-DNA/RNA, contrary to chalcones forms (dominant at pH 7), which did not show binding to polynucleotides. Flavylium cation intercalated into ds-DNAs at variance to dominant groove aggregation within ds-RNA, which was reported by RNA-specific bisignate induced CD spectrum (ICD) bands. The intrinsically negligible fluorescence of flavylium was strongly increased upon the addition of DNA or RNA, whereby both the fluorescence intensity and emission lifetimes of complexes differed considerably: the strongest emission increase was observed for AU-RNA (detection limit estimated to 10 nM) followed by AT-DNAs and the much weaker effect of GC-DNAs. Both fluorescence sensitivity on the ds-DNA/RNA secondary structure and sequence-selective ICD bands make the flavylium–chalcones system an intriguing pH-switchable new probe for distinguishing between various polynucleotide sequences.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNCrnolatac, IvoGiestas, LetíciaHorvat, GordanParola, António JorgePiantanida, Ivo2021-03-29T22:24:58Z2020-12-132020-12-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://hdl.handle.net/10362/114694eng2227-9040PURE: 28793625https://doi.org/10.3390/chemosensors8040129info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:57:23Zoai:run.unl.pt:10362/114694Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:42:36.706210Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA
title Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA
spellingShingle Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA
Crnolatac, Ivo
Chalcones
DNA/RNA binding
Flavylium cation
Fluorescence, circular dichroism
pH control
Analytical Chemistry
Physical and Theoretical Chemistry
title_short Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA
title_full Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA
title_fullStr Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA
title_full_unstemmed Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA
title_sort Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA
author Crnolatac, Ivo
author_facet Crnolatac, Ivo
Giestas, Letícia
Horvat, Gordan
Parola, António Jorge
Piantanida, Ivo
author_role author
author2 Giestas, Letícia
Horvat, Gordan
Parola, António Jorge
Piantanida, Ivo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Crnolatac, Ivo
Giestas, Letícia
Horvat, Gordan
Parola, António Jorge
Piantanida, Ivo
dc.subject.por.fl_str_mv Chalcones
DNA/RNA binding
Flavylium cation
Fluorescence, circular dichroism
pH control
Analytical Chemistry
Physical and Theoretical Chemistry
topic Chalcones
DNA/RNA binding
Flavylium cation
Fluorescence, circular dichroism
pH control
Analytical Chemistry
Physical and Theoretical Chemistry
description UIDB/50006/2020 ROTEIRO/0031/2013-PINFRA/22161/2016 HrZZ IP-2018-01-5475
publishDate 2020
dc.date.none.fl_str_mv 2020-12-13
2020-12-13T00:00:00Z
2021-03-29T22:24:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/114694
url http://hdl.handle.net/10362/114694
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2227-9040
PURE: 28793625
https://doi.org/10.3390/chemosensors8040129
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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