Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/114694 |
Resumo: | UIDB/50006/2020 ROTEIRO/0031/2013-PINFRA/22161/2016 HrZZ IP-2018-01-5475 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNAChalconesDNA/RNA bindingFlavylium cationFluorescence, circular dichroismpH controlAnalytical ChemistryPhysical and Theoretical ChemistryUIDB/50006/2020 ROTEIRO/0031/2013-PINFRA/22161/2016 HrZZ IP-2018-01-5475The interaction of 4′-(N,N-dimethylamino)-6-hydroxyflavylium cation with double stranded (ds-) DNA/RNA was studied by UV/Vis spectrophotometry, circular dichroism (CD), and also steady-state and time-resolved emission spectroscopies at neutral and weakly acidic conditions. At pH 5, the studied molecule, in its flavylium cationic form, showed considerable binding affinities (5 < logKs < 6) for all ds-DNA/RNA, contrary to chalcones forms (dominant at pH 7), which did not show binding to polynucleotides. Flavylium cation intercalated into ds-DNAs at variance to dominant groove aggregation within ds-RNA, which was reported by RNA-specific bisignate induced CD spectrum (ICD) bands. The intrinsically negligible fluorescence of flavylium was strongly increased upon the addition of DNA or RNA, whereby both the fluorescence intensity and emission lifetimes of complexes differed considerably: the strongest emission increase was observed for AU-RNA (detection limit estimated to 10 nM) followed by AT-DNAs and the much weaker effect of GC-DNAs. Both fluorescence sensitivity on the ds-DNA/RNA secondary structure and sequence-selective ICD bands make the flavylium–chalcones system an intriguing pH-switchable new probe for distinguishing between various polynucleotide sequences.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNCrnolatac, IvoGiestas, LetíciaHorvat, GordanParola, António JorgePiantanida, Ivo2021-03-29T22:24:58Z2020-12-132020-12-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://hdl.handle.net/10362/114694eng2227-9040PURE: 28793625https://doi.org/10.3390/chemosensors8040129info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:57:23Zoai:run.unl.pt:10362/114694Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:42:36.706210Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA |
title |
Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA |
spellingShingle |
Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA Crnolatac, Ivo Chalcones DNA/RNA binding Flavylium cation Fluorescence, circular dichroism pH control Analytical Chemistry Physical and Theoretical Chemistry |
title_short |
Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA |
title_full |
Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA |
title_fullStr |
Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA |
title_full_unstemmed |
Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA |
title_sort |
Flavylium dye as pH-tunable fluorescent and CD probe for double-stranded DNA and RNA |
author |
Crnolatac, Ivo |
author_facet |
Crnolatac, Ivo Giestas, Letícia Horvat, Gordan Parola, António Jorge Piantanida, Ivo |
author_role |
author |
author2 |
Giestas, Letícia Horvat, Gordan Parola, António Jorge Piantanida, Ivo |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
LAQV@REQUIMTE DQ - Departamento de Química RUN |
dc.contributor.author.fl_str_mv |
Crnolatac, Ivo Giestas, Letícia Horvat, Gordan Parola, António Jorge Piantanida, Ivo |
dc.subject.por.fl_str_mv |
Chalcones DNA/RNA binding Flavylium cation Fluorescence, circular dichroism pH control Analytical Chemistry Physical and Theoretical Chemistry |
topic |
Chalcones DNA/RNA binding Flavylium cation Fluorescence, circular dichroism pH control Analytical Chemistry Physical and Theoretical Chemistry |
description |
UIDB/50006/2020 ROTEIRO/0031/2013-PINFRA/22161/2016 HrZZ IP-2018-01-5475 |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-13 2020-12-13T00:00:00Z 2021-03-29T22:24:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/114694 |
url |
http://hdl.handle.net/10362/114694 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2227-9040 PURE: 28793625 https://doi.org/10.3390/chemosensors8040129 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799138037796438016 |