Manipulation of the Quorum Sensing Signal AI-2 Affects the Antibiotic-Treated Gut Microbiota
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/658 |
Resumo: | The mammalian gut microbiota harbors a diverse ecosystem where hundreds of bacterial species interact with each other and their host. Given that bacteria use signals to communicate and regulate group behaviors (quorum sensing), we asked whether such communication between different commensal species can influence the interactions occurring in this environment. We engineered the enteric bacterium, Escherichia coli, to manipulate the levels of the interspecies quorum sensing signal, autoinducer-2 (AI-2), in the mouse intestine and investigated the effect upon antibiotic-induced gut microbiota dysbiosis. E. coli that increased intestinal AI-2 levels altered the composition of the antibiotic-treated gut microbiota, favoring the expansion of the Firmicutes phylum. This significantly increased the Firmicutes/Bacteroidetes ratio, to oppose the strong effect of the antibiotic, which had almost cleared the Firmicutes. This demonstrates that AI-2 levels influence the abundance of the major phyla of the gut microbiota, the balance of which is known to influence human health. |
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Manipulation of the Quorum Sensing Signal AI-2 Affects the Antibiotic-Treated Gut MicrobiotaAnimalsAnti-Bacterial AgentsBacteroidetesEscherichia coliHomoserineIntestinesLactonesMaleMiceMice, Inbred C57BLMicrobiotaStaphylococcaceaeStreptomycinQuorum SensingThe mammalian gut microbiota harbors a diverse ecosystem where hundreds of bacterial species interact with each other and their host. Given that bacteria use signals to communicate and regulate group behaviors (quorum sensing), we asked whether such communication between different commensal species can influence the interactions occurring in this environment. We engineered the enteric bacterium, Escherichia coli, to manipulate the levels of the interspecies quorum sensing signal, autoinducer-2 (AI-2), in the mouse intestine and investigated the effect upon antibiotic-induced gut microbiota dysbiosis. E. coli that increased intestinal AI-2 levels altered the composition of the antibiotic-treated gut microbiota, favoring the expansion of the Firmicutes phylum. This significantly increased the Firmicutes/Bacteroidetes ratio, to oppose the strong effect of the antibiotic, which had almost cleared the Firmicutes. This demonstrates that AI-2 levels influence the abundance of the major phyla of the gut microbiota, the balance of which is known to influence human health.Howard Hughes Medical Institute grants: (International Early Career Scientist, HHMI 55007436); Fundação para a Ciência e Tecnologia grants: (PTDC/BIA-EVF/118075/2010, RECI/IMI-IMU/0038/2012); Ministerio de Ciencia e Innovacion grants: (MICINN, SAF2011-29458).Cell PressARCAThompson, Jessica AnnOliveira, Rita AlmeidaDjukovic, AnaUbeda, CarlesXavier, Karina Bivar2016-06-21T14:06:27Z2015-03-242015-03-24T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.7/658engJessica Ann Thompson, Rita Almeida Oliveira, Ana Djukovic, Carles Ubeda, Karina Bivar Xavier, Manipulation of the Quorum Sensing Signal AI-2 Affects the Antibiotic-Treated Gut Microbiota, Cell Reports, Volume 10, Issue 11, 24 March 2015, Pages 1861-1871, ISSN 2211-1247, http://dx.doi.org/10.1016/j.celrep.2015.02.049. (http://www.sciencedirect.com/science/article/pii/S2211124715002077)10.1016/j.celrep.2015.02.049info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:03Zoai:arca.igc.gulbenkian.pt:10400.7/658Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:53.881449Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Manipulation of the Quorum Sensing Signal AI-2 Affects the Antibiotic-Treated Gut Microbiota |
title |
Manipulation of the Quorum Sensing Signal AI-2 Affects the Antibiotic-Treated Gut Microbiota |
spellingShingle |
Manipulation of the Quorum Sensing Signal AI-2 Affects the Antibiotic-Treated Gut Microbiota Thompson, Jessica Ann Animals Anti-Bacterial Agents Bacteroidetes Escherichia coli Homoserine Intestines Lactones Male Mice Mice, Inbred C57BL Microbiota Staphylococcaceae Streptomycin Quorum Sensing |
title_short |
Manipulation of the Quorum Sensing Signal AI-2 Affects the Antibiotic-Treated Gut Microbiota |
title_full |
Manipulation of the Quorum Sensing Signal AI-2 Affects the Antibiotic-Treated Gut Microbiota |
title_fullStr |
Manipulation of the Quorum Sensing Signal AI-2 Affects the Antibiotic-Treated Gut Microbiota |
title_full_unstemmed |
Manipulation of the Quorum Sensing Signal AI-2 Affects the Antibiotic-Treated Gut Microbiota |
title_sort |
Manipulation of the Quorum Sensing Signal AI-2 Affects the Antibiotic-Treated Gut Microbiota |
author |
Thompson, Jessica Ann |
author_facet |
Thompson, Jessica Ann Oliveira, Rita Almeida Djukovic, Ana Ubeda, Carles Xavier, Karina Bivar |
author_role |
author |
author2 |
Oliveira, Rita Almeida Djukovic, Ana Ubeda, Carles Xavier, Karina Bivar |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Thompson, Jessica Ann Oliveira, Rita Almeida Djukovic, Ana Ubeda, Carles Xavier, Karina Bivar |
dc.subject.por.fl_str_mv |
Animals Anti-Bacterial Agents Bacteroidetes Escherichia coli Homoserine Intestines Lactones Male Mice Mice, Inbred C57BL Microbiota Staphylococcaceae Streptomycin Quorum Sensing |
topic |
Animals Anti-Bacterial Agents Bacteroidetes Escherichia coli Homoserine Intestines Lactones Male Mice Mice, Inbred C57BL Microbiota Staphylococcaceae Streptomycin Quorum Sensing |
description |
The mammalian gut microbiota harbors a diverse ecosystem where hundreds of bacterial species interact with each other and their host. Given that bacteria use signals to communicate and regulate group behaviors (quorum sensing), we asked whether such communication between different commensal species can influence the interactions occurring in this environment. We engineered the enteric bacterium, Escherichia coli, to manipulate the levels of the interspecies quorum sensing signal, autoinducer-2 (AI-2), in the mouse intestine and investigated the effect upon antibiotic-induced gut microbiota dysbiosis. E. coli that increased intestinal AI-2 levels altered the composition of the antibiotic-treated gut microbiota, favoring the expansion of the Firmicutes phylum. This significantly increased the Firmicutes/Bacteroidetes ratio, to oppose the strong effect of the antibiotic, which had almost cleared the Firmicutes. This demonstrates that AI-2 levels influence the abundance of the major phyla of the gut microbiota, the balance of which is known to influence human health. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-24 2015-03-24T00:00:00Z 2016-06-21T14:06:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/658 |
url |
http://hdl.handle.net/10400.7/658 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Jessica Ann Thompson, Rita Almeida Oliveira, Ana Djukovic, Carles Ubeda, Karina Bivar Xavier, Manipulation of the Quorum Sensing Signal AI-2 Affects the Antibiotic-Treated Gut Microbiota, Cell Reports, Volume 10, Issue 11, 24 March 2015, Pages 1861-1871, ISSN 2211-1247, http://dx.doi.org/10.1016/j.celrep.2015.02.049. (http://www.sciencedirect.com/science/article/pii/S2211124715002077) 10.1016/j.celrep.2015.02.049 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Cell Press |
publisher.none.fl_str_mv |
Cell Press |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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