Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells

Detalhes bibliográficos
Autor(a) principal: Cal, Pedro M.S.D.
Data de Publicação: 2017
Outros Autores: Sieglitz, Florian, Santos, Fábio, Parente Carvalho, Cátia, Guerreiro, Ana, Bertoldo, Jean B., Pischel, Uwe, Gois, Pedro M. P., Bernardes, Gonçalo J. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/51441
Resumo: This journal is © The Royal Society of Chemistry 2017. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
id RCAP_625a1a7c4e9a69c0c63383928b11f0ff
oai_identifier_str oai:repositorio.ul.pt:10451/51441
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cellsThis journal is © The Royal Society of Chemistry 2017. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.Fluorophores are indispensable for imaging biological processes. We report the design and synthesis of azide-tagged boronic acid salicylidenehydrazone (BASHY) dyes and their use for site-selective labelling of Annexin V. The Annexin V-BASHY conjugate maintained function and fluorescence as demonstrated by the targeted detection of apoptotic cells.We thank FCT Portugal (Doctoral Fellowship, SFRH/BD/94779/2013 to F. M. F. S., Postdoctoral Fellowship, SFRH/BPD/103172/2014 to P. M. S. D. C.; projects PTDC/QUI-QUI/118315/2010 and PTDC/BBB BQB/0506/2012; PTDC/QEQ-QOR/1434/2014: PTDC/SAUFAR/119389/2010; FCT Investigator to G. J. L. B. and P. M. P. G.; iMed.ULisboa grant UID/DTP/04138/2013), EU (Marie-Curie CIG to G. J. L. B.; Marie-Sklodowska Curie ITN ProteinConjugates to G. J. L. B. and P. M. P. G.), DFG (SI 2117/1-1 to F. S.), CNPq Brazil (fellowship 200456/2015-6 to J. B. B.); Ministerio de Economía y Competitividad, Madrid, Spain (grant CTQ2014-54729-C2-1-P), Junta de Andalucía (grant P12-FQM-2140) and the EPSRC (G. J. L. B.) for financial support. G. J. L. B. is a Royal Society University Research Fellow and the recipient of a European Research Council Starting Grant (TagIt).Royal Society of ChemistryRepositório da Universidade de LisboaCal, Pedro M.S.D.Sieglitz, FlorianSantos, FábioParente Carvalho, CátiaGuerreiro, AnaBertoldo, Jean B.Pischel, UweGois, Pedro M. P.Bernardes, Gonçalo J. L.2022-02-21T18:00:39Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51441engChem. Commun., 2017, 53, 368-3711359-734510.1039/C6CC08671C1364-548Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:56:08Zoai:repositorio.ul.pt:10451/51441Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:43.942693Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells
title Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells
spellingShingle Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells
Cal, Pedro M.S.D.
title_short Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells
title_full Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells
title_fullStr Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells
title_full_unstemmed Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells
title_sort Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells
author Cal, Pedro M.S.D.
author_facet Cal, Pedro M.S.D.
Sieglitz, Florian
Santos, Fábio
Parente Carvalho, Cátia
Guerreiro, Ana
Bertoldo, Jean B.
Pischel, Uwe
Gois, Pedro M. P.
Bernardes, Gonçalo J. L.
author_role author
author2 Sieglitz, Florian
Santos, Fábio
Parente Carvalho, Cátia
Guerreiro, Ana
Bertoldo, Jean B.
Pischel, Uwe
Gois, Pedro M. P.
Bernardes, Gonçalo J. L.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Cal, Pedro M.S.D.
Sieglitz, Florian
Santos, Fábio
Parente Carvalho, Cátia
Guerreiro, Ana
Bertoldo, Jean B.
Pischel, Uwe
Gois, Pedro M. P.
Bernardes, Gonçalo J. L.
description This journal is © The Royal Society of Chemistry 2017. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2022-02-21T18:00:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/51441
url http://hdl.handle.net/10451/51441
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chem. Commun., 2017, 53, 368-371
1359-7345
10.1039/C6CC08671C
1364-548X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799134577119199232

Registros relacionados