Mössbauer study of the native, reduced and substrate‐reacted Desulfovibrio gigas aldehyde oxido‐reductase

Detalhes bibliográficos
Autor(a) principal: Barata, Belarmino A. S.
Data de Publicação: 1992
Outros Autores: Liang, Jin, Moura, Isabel, LeGall, Jean, Moura, José J. G., Hanh Huynh, Boi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/80774
Resumo: NIGMS NIH HHS (GM41482; GM 32187)
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spelling Mössbauer study of the native, reduced and substrate‐reacted Desulfovibrio gigas aldehyde oxido‐reductaseBiochemistryNIGMS NIH HHS (GM41482; GM 32187)The Desulfovibrio gigas aldehyde oxido‐reductase contains molybdenum and iron‐sulfur clusters. Mössbauer spectroscopy was used to characterize the iron‐sulfur clusters. Spectra of the enzyme in its oxidized, partially reduced and benzaldehyde‐reacted states were recorded at different temperatures and applied magnetic fields. All the iron atoms in D. gigas aldehyde oxido‐reductase are organized as [2Fe‐2S] clusters. In the oxydized enzyme, the clusters are diamagnetic and exhibit a single quadrupole doublet with parameters (ΔEQ= 0.62 ± 0.02 mm/s and δ= 0.27 ± 0.01 mm/s) typical for the [2Fe‐2S]2+ state. Mössbauer spectra of the reduced clusters also show the characteristics of a [2Fe‐2S]1+ cluster and can be explained by a spin‐coupling model proposed for the [2Fe‐2S] cluster where a high‐spin ferrous ion (S= 2) is antiferromagnetically coupled to a high‐spin ferric ion (S= 5/2) to form a S= 1/2 system. Two ferrous sites with different ΔEQ values (3.42 mm/s and 2.93 mm/s at 85 K) are observed for the reduced enzyme, indicating the presence of two types of [2Fe‐2S] clusters in the D. gigas enzyme. Taking this observation together with the re‐evaluated value of iron content (3.5 ± 0.1 Fe/molecule), it is concluded that, similar to other Mo‐hydroxylases, the D. gigas aldehyde oxido‐reductase also contains two spectroscopically distinguishable [2Fe‐2S] clusters.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNBarata, Belarmino A. S.Liang, JinMoura, IsabelLeGall, JeanMoura, José J. G.Hanh Huynh, Boi2019-09-10T22:49:49Z1992-01-011992-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6application/pdfhttp://hdl.handle.net/10362/80774eng0014-2956PURE: 14639904http://www.scopus.com/inward/record.url?scp=0026610349&partnerID=8YFLogxKhttps://doi.org/10.1111/j.1432-1033.1992.tb16693.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:35:45Zoai:run.unl.pt:10362/80774Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:35:56.016798Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Mössbauer study of the native, reduced and substrate‐reacted Desulfovibrio gigas aldehyde oxido‐reductase
title Mössbauer study of the native, reduced and substrate‐reacted Desulfovibrio gigas aldehyde oxido‐reductase
spellingShingle Mössbauer study of the native, reduced and substrate‐reacted Desulfovibrio gigas aldehyde oxido‐reductase
Barata, Belarmino A. S.
Biochemistry
title_short Mössbauer study of the native, reduced and substrate‐reacted Desulfovibrio gigas aldehyde oxido‐reductase
title_full Mössbauer study of the native, reduced and substrate‐reacted Desulfovibrio gigas aldehyde oxido‐reductase
title_fullStr Mössbauer study of the native, reduced and substrate‐reacted Desulfovibrio gigas aldehyde oxido‐reductase
title_full_unstemmed Mössbauer study of the native, reduced and substrate‐reacted Desulfovibrio gigas aldehyde oxido‐reductase
title_sort Mössbauer study of the native, reduced and substrate‐reacted Desulfovibrio gigas aldehyde oxido‐reductase
author Barata, Belarmino A. S.
author_facet Barata, Belarmino A. S.
Liang, Jin
Moura, Isabel
LeGall, Jean
Moura, José J. G.
Hanh Huynh, Boi
author_role author
author2 Liang, Jin
Moura, Isabel
LeGall, Jean
Moura, José J. G.
Hanh Huynh, Boi
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Barata, Belarmino A. S.
Liang, Jin
Moura, Isabel
LeGall, Jean
Moura, José J. G.
Hanh Huynh, Boi
dc.subject.por.fl_str_mv Biochemistry
topic Biochemistry
description NIGMS NIH HHS (GM41482; GM 32187)
publishDate 1992
dc.date.none.fl_str_mv 1992-01-01
1992-01-01T00:00:00Z
2019-09-10T22:49:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/80774
url http://hdl.handle.net/10362/80774
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0014-2956
PURE: 14639904
http://www.scopus.com/inward/record.url?scp=0026610349&partnerID=8YFLogxK
https://doi.org/10.1111/j.1432-1033.1992.tb16693.x
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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