Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/143377 |
Resumo: | Sweat is a potential biological fluid for the non-invasive analytical assessment of diverse molecules, including biomarkers. Not with standing, the sampling methodology is critical, and it must be assessed prior to using sweat for clinical diagnosis. In the current work, the analytical methodology was further developed taking into account the sampling step, in view of the identification and level variations of sweat components that have potential to be stress biomarkers using separation by liquid chromatography and detection by tandem mass spectrometry, in order to attain a screening profile of 26 molecules in just one stage. As such, the molecule identification was used as a test for the evaluation of the sampling procedures, including the location on the body, using patches for long-term sampling and vials for direct sampling, through a qualitative approach. From this evaluation it was possible to conclude that the sampling may be performed on the chest or back skin. Additionally, possible interference was evaluated. The long-term sampling with patches can be used under both rest and exercise conditions with variation of the detected molecule’s levels. The direct sampling, using vials, has the advantage of not having interferences but the disadvantage of only being effective after exercise in order to have enough sample for sweat analysis. |
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Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker DetectionLC-MS/MSstressbiomarkerssamplingsweatblood analysisAnalytical ChemistrySDG 3 - Good Health and Well-beingSweat is a potential biological fluid for the non-invasive analytical assessment of diverse molecules, including biomarkers. Not with standing, the sampling methodology is critical, and it must be assessed prior to using sweat for clinical diagnosis. In the current work, the analytical methodology was further developed taking into account the sampling step, in view of the identification and level variations of sweat components that have potential to be stress biomarkers using separation by liquid chromatography and detection by tandem mass spectrometry, in order to attain a screening profile of 26 molecules in just one stage. As such, the molecule identification was used as a test for the evaluation of the sampling procedures, including the location on the body, using patches for long-term sampling and vials for direct sampling, through a qualitative approach. From this evaluation it was possible to conclude that the sampling may be performed on the chest or back skin. Additionally, possible interference was evaluated. The long-term sampling with patches can be used under both rest and exercise conditions with variation of the detected molecule’s levels. The direct sampling, using vials, has the advantage of not having interferences but the disadvantage of only being effective after exercise in order to have enough sample for sweat analysis.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNNunes, Maria JoãoMoura, José J. G.Noronha, João PauloBranco, Luís CobraSamhan-Arias, AlejandroSousa, João P.Rouco, CarlosCordas, Cristina M.2022-08-30T22:23:06Z2022-04-162022-04-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17application/pdfhttp://hdl.handle.net/10362/143377eng2673-4532PURE: 43388729https://doi.org/10.3390/analytica3020013info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:21:42Zoai:run.unl.pt:10362/143377Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:50:54.643648Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection |
title |
Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection |
spellingShingle |
Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection Nunes, Maria João LC-MS/MS stress biomarkers sampling sweat blood analysis Analytical Chemistry SDG 3 - Good Health and Well-being |
title_short |
Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection |
title_full |
Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection |
title_fullStr |
Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection |
title_full_unstemmed |
Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection |
title_sort |
Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection |
author |
Nunes, Maria João |
author_facet |
Nunes, Maria João Moura, José J. G. Noronha, João Paulo Branco, Luís Cobra Samhan-Arias, Alejandro Sousa, João P. Rouco, Carlos Cordas, Cristina M. |
author_role |
author |
author2 |
Moura, José J. G. Noronha, João Paulo Branco, Luís Cobra Samhan-Arias, Alejandro Sousa, João P. Rouco, Carlos Cordas, Cristina M. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
LAQV@REQUIMTE DQ - Departamento de Química RUN |
dc.contributor.author.fl_str_mv |
Nunes, Maria João Moura, José J. G. Noronha, João Paulo Branco, Luís Cobra Samhan-Arias, Alejandro Sousa, João P. Rouco, Carlos Cordas, Cristina M. |
dc.subject.por.fl_str_mv |
LC-MS/MS stress biomarkers sampling sweat blood analysis Analytical Chemistry SDG 3 - Good Health and Well-being |
topic |
LC-MS/MS stress biomarkers sampling sweat blood analysis Analytical Chemistry SDG 3 - Good Health and Well-being |
description |
Sweat is a potential biological fluid for the non-invasive analytical assessment of diverse molecules, including biomarkers. Not with standing, the sampling methodology is critical, and it must be assessed prior to using sweat for clinical diagnosis. In the current work, the analytical methodology was further developed taking into account the sampling step, in view of the identification and level variations of sweat components that have potential to be stress biomarkers using separation by liquid chromatography and detection by tandem mass spectrometry, in order to attain a screening profile of 26 molecules in just one stage. As such, the molecule identification was used as a test for the evaluation of the sampling procedures, including the location on the body, using patches for long-term sampling and vials for direct sampling, through a qualitative approach. From this evaluation it was possible to conclude that the sampling may be performed on the chest or back skin. Additionally, possible interference was evaluated. The long-term sampling with patches can be used under both rest and exercise conditions with variation of the detected molecule’s levels. The direct sampling, using vials, has the advantage of not having interferences but the disadvantage of only being effective after exercise in order to have enough sample for sweat analysis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-30T22:23:06Z 2022-04-16 2022-04-16T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/143377 |
url |
http://hdl.handle.net/10362/143377 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2673-4532 PURE: 43388729 https://doi.org/10.3390/analytica3020013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
17 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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