Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry

Detalhes bibliográficos
Autor(a) principal: Taborda, Tiago
Data de Publicação: 2021
Outros Autores: Moniz, Patricia, Reis, Alberto, Silva, Teresa Lopes da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.9/3624
Resumo: ABSTRACT: Crypthecodinium cohnii growth was studied on pure carbon sources (glucose, acetate, glycerol) and low-cost complex carbon sources (sugarcane molasses, crude glycerol and vinegar effluent) for lipid and DHA production. Among the pure substrates, glucose induced the highest lipid content (14.75% w/w DCW) and DHA content (7.14 mg g(-1) DCW). Among the low-cost substrates, the highest lipid and DHA content were observed for the crude glycerol assay (14.7% w/w DCW and 6.56 mg g(-1), respectively). Molasses induced the highest proportion of DHA of total fatty acids (49.58% w/w TFA) among all the substrates studied. Flow cytometric analysis revealed that the vinegar effluent induced the highest proportion of C. cohnii cells with injured membrane (92.8%). These results foresee the possibility of using these low-cost substrates at a larger scale for C. cohnii DHA and biodiesel production, aiming at zero wastes and process costs reduction.
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spelling Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometryMicroalgaeAlternative carbon sourceSubstratesLipidsFlow cytometryABSTRACT: Crypthecodinium cohnii growth was studied on pure carbon sources (glucose, acetate, glycerol) and low-cost complex carbon sources (sugarcane molasses, crude glycerol and vinegar effluent) for lipid and DHA production. Among the pure substrates, glucose induced the highest lipid content (14.75% w/w DCW) and DHA content (7.14 mg g(-1) DCW). Among the low-cost substrates, the highest lipid and DHA content were observed for the crude glycerol assay (14.7% w/w DCW and 6.56 mg g(-1), respectively). Molasses induced the highest proportion of DHA of total fatty acids (49.58% w/w TFA) among all the substrates studied. Flow cytometric analysis revealed that the vinegar effluent induced the highest proportion of C. cohnii cells with injured membrane (92.8%). These results foresee the possibility of using these low-cost substrates at a larger scale for C. cohnii DHA and biodiesel production, aiming at zero wastes and process costs reduction.SpringerRepositório do LNEGTaborda, TiagoMoniz, PatriciaReis, AlbertoSilva, Teresa Lopes da2021-12-03T12:15:47Z2021-02-01T00:00:00Z2021-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.9/3624engTaborda, Tiago... [et.al.] - Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry. In: Journal of Applied Phycology, 2021, Vol. 33 (1), p. 263-2740921-897110.1007/s10811-020-02304-z1573-5176info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-06T12:29:19Zoai:repositorio.lneg.pt:10400.9/3624Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:36:50.089599Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry
title Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry
spellingShingle Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry
Taborda, Tiago
Microalgae
Alternative carbon source
Substrates
Lipids
Flow cytometry
title_short Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry
title_full Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry
title_fullStr Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry
title_full_unstemmed Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry
title_sort Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry
author Taborda, Tiago
author_facet Taborda, Tiago
Moniz, Patricia
Reis, Alberto
Silva, Teresa Lopes da
author_role author
author2 Moniz, Patricia
Reis, Alberto
Silva, Teresa Lopes da
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório do LNEG
dc.contributor.author.fl_str_mv Taborda, Tiago
Moniz, Patricia
Reis, Alberto
Silva, Teresa Lopes da
dc.subject.por.fl_str_mv Microalgae
Alternative carbon source
Substrates
Lipids
Flow cytometry
topic Microalgae
Alternative carbon source
Substrates
Lipids
Flow cytometry
description ABSTRACT: Crypthecodinium cohnii growth was studied on pure carbon sources (glucose, acetate, glycerol) and low-cost complex carbon sources (sugarcane molasses, crude glycerol and vinegar effluent) for lipid and DHA production. Among the pure substrates, glucose induced the highest lipid content (14.75% w/w DCW) and DHA content (7.14 mg g(-1) DCW). Among the low-cost substrates, the highest lipid and DHA content were observed for the crude glycerol assay (14.7% w/w DCW and 6.56 mg g(-1), respectively). Molasses induced the highest proportion of DHA of total fatty acids (49.58% w/w TFA) among all the substrates studied. Flow cytometric analysis revealed that the vinegar effluent induced the highest proportion of C. cohnii cells with injured membrane (92.8%). These results foresee the possibility of using these low-cost substrates at a larger scale for C. cohnii DHA and biodiesel production, aiming at zero wastes and process costs reduction.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-03T12:15:47Z
2021-02-01T00:00:00Z
2021-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.9/3624
url http://hdl.handle.net/10400.9/3624
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Taborda, Tiago... [et.al.] - Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry. In: Journal of Applied Phycology, 2021, Vol. 33 (1), p. 263-274
0921-8971
10.1007/s10811-020-02304-z
1573-5176
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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