Escherichia coli RIC is able to donate iron to iron-sulfur clusters

Detalhes bibliográficos
Autor(a) principal: Nobre, Lígia S.
Data de Publicação: 2014
Outros Autores: Garcia-Serres, Ricardo, Todorovic, Smilja, Hildebrandt, Peter, Teixeira, Miguel, Latour, Jean Marc, Saraiva, Lígia M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/93031
Resumo: WOS:000336863900120
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spelling Escherichia coli RIC is able to donate iron to iron-sulfur clustersAgricultural and Biological Sciences(all)Biochemistry, Genetics and Molecular Biology(all)Medicine(all)WOS:000336863900120Escherichia coli RIC (Repair of Iron Centers) is a diiron protein previously reported to be involved in the repair of iron-sulfur proteins damaged by oxidative or nitrosative stresses, and proposed to act as an iron donor. This possible role of RIC was now examined specifically by evaluating its ability to donate iron ions to apo-iron-sulfur proteins, determining the iron binding constants and assessing the lability of its iron ions. We show, by UV-visible, EPR and resonance Raman spectroscopies that RIC may participate in the synthesis of an iron-sulfur cluster in the apo-forms of the spinach ferredoxin and IscU when in the presence of the sulfide donating system IscS and L-cysteine. Iron binding assays allowed determining the as-isolated and fully reduced RIC dissociation constants for the ferric and ferrous iron of 10-27 M and 10-13 M, respectively. Mössbauer studies revealed that the RIC iron ions are labile, namely when the center is in the mixed-valence redox form as compared with the (μ-oxo) diferric one. Altogether, these results suggest that RIC is capable of delivering iron for the formation of iron-sulfur clusters.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNNobre, Lígia S.Garcia-Serres, RicardoTodorovic, SmiljaHildebrandt, PeterTeixeira, MiguelLatour, Jean MarcSaraiva, Lígia M.2020-02-19T23:25:19Z2014-04-012014-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/93031eng1932-6203PURE: 1766456https://doi.org/10.1371/journal.pone.0095222info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:41:34Zoai:run.unl.pt:10362/93031Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:37:40.262511Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Escherichia coli RIC is able to donate iron to iron-sulfur clusters
title Escherichia coli RIC is able to donate iron to iron-sulfur clusters
spellingShingle Escherichia coli RIC is able to donate iron to iron-sulfur clusters
Nobre, Lígia S.
Agricultural and Biological Sciences(all)
Biochemistry, Genetics and Molecular Biology(all)
Medicine(all)
title_short Escherichia coli RIC is able to donate iron to iron-sulfur clusters
title_full Escherichia coli RIC is able to donate iron to iron-sulfur clusters
title_fullStr Escherichia coli RIC is able to donate iron to iron-sulfur clusters
title_full_unstemmed Escherichia coli RIC is able to donate iron to iron-sulfur clusters
title_sort Escherichia coli RIC is able to donate iron to iron-sulfur clusters
author Nobre, Lígia S.
author_facet Nobre, Lígia S.
Garcia-Serres, Ricardo
Todorovic, Smilja
Hildebrandt, Peter
Teixeira, Miguel
Latour, Jean Marc
Saraiva, Lígia M.
author_role author
author2 Garcia-Serres, Ricardo
Todorovic, Smilja
Hildebrandt, Peter
Teixeira, Miguel
Latour, Jean Marc
Saraiva, Lígia M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Nobre, Lígia S.
Garcia-Serres, Ricardo
Todorovic, Smilja
Hildebrandt, Peter
Teixeira, Miguel
Latour, Jean Marc
Saraiva, Lígia M.
dc.subject.por.fl_str_mv Agricultural and Biological Sciences(all)
Biochemistry, Genetics and Molecular Biology(all)
Medicine(all)
topic Agricultural and Biological Sciences(all)
Biochemistry, Genetics and Molecular Biology(all)
Medicine(all)
description WOS:000336863900120
publishDate 2014
dc.date.none.fl_str_mv 2014-04-01
2014-04-01T00:00:00Z
2020-02-19T23:25:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/93031
url http://hdl.handle.net/10362/93031
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
PURE: 1766456
https://doi.org/10.1371/journal.pone.0095222
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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