Expression of tumour-related Rac1b antagonizes B-Raf-induced senescence in colorectal cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.18/3424 |
Resumo: | Erratum Cancer Lett. 2022 Dec 1;550:215936. doi: 10.1016/j.canlet.2022.215936. Epub 2022 Oct 11. |
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Expression of tumour-related Rac1b antagonizes B-Raf-induced senescence in colorectal cellsVias de Transdução de Sinal e Patologias AssociadasCancro ColoretalRac1bSignallingSenescenceB-RafColorectal CancerTumor ProgressionErratum Cancer Lett. 2022 Dec 1;550:215936. doi: 10.1016/j.canlet.2022.215936. Epub 2022 Oct 11.Mutations in the BRAF oncogene have been identified as a tumor-initiating genetic event in mainly melanoma, thyroid and colon cancer, resulting in an initial proliferative stimulus that is followed by a growth arrest period known as oncogene-induced senescence (OIS). It remains unknown what triggers subsequent escape from OIS to allow further tumor progression. A previous analysis revealed that around 80% of colorectal tumors carrying a mutation in BRAF also overexpress splice variant Rac1b. We used normal NCM460 colonocytes as a model to express oncogenic B-Raf-V600E in the presence or absence of co-transfected Rac1b and then analyzed the effect on expression of senescence markers. When oncogenic B-Raf-V600E was expressed we observed the induction of the senescence-associated β-galactosidase and of the cell-cycle inhibitors p14, p15 and p21 whereas proliferation marker Ki67 was suppressed. Upon co-expression of splice variant Rac1b, but not of Rac1, the B-Raf-induced senescence phenotype was reverted and expression of the cell-cycle inhibitors downregulated in a reactive oxygen-species dependent manner. We thus provide evidence that co-expression of splice variant Rac1b counteracts B-Raf-induced senescence, indicating the selection for increased Rac1b expression as one potential mechanism by which colorectal tumor cells can escape from B-Raf-induced OIS.Fundação para a Ciência e Tecnologia, Portugal (center grant to BioISI (UID/MULTI/04046/2013), contract ‘FCT Investigator’ to P.M. and fellowship SFRH/BPD/ 94322/2013 to P.BElsevierRepositório Científico do Instituto Nacional de SaúdeHenriques, Andreia FABarros, PatríciaMoyer, MaryMatos, PauloJordan, Peter2019-01-01T01:30:10Z2015-12-282015-12-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.18/3424engCancer Lett. 2015 Dec 28;369(2):368-75. doi: 10.1016/j.canlet.2015.08.027. Epub 2015 Sep 10304-383510.1016/j.canlet.2015.08.027info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:43Zoai:repositorio.insa.pt:10400.18/3424Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:38:12.388882Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Expression of tumour-related Rac1b antagonizes B-Raf-induced senescence in colorectal cells |
| title |
Expression of tumour-related Rac1b antagonizes B-Raf-induced senescence in colorectal cells |
| spellingShingle |
Expression of tumour-related Rac1b antagonizes B-Raf-induced senescence in colorectal cells Henriques, Andreia FA Vias de Transdução de Sinal e Patologias Associadas Cancro Coloretal Rac1b Signalling Senescence B-Raf Colorectal Cancer Tumor Progression |
| title_short |
Expression of tumour-related Rac1b antagonizes B-Raf-induced senescence in colorectal cells |
| title_full |
Expression of tumour-related Rac1b antagonizes B-Raf-induced senescence in colorectal cells |
| title_fullStr |
Expression of tumour-related Rac1b antagonizes B-Raf-induced senescence in colorectal cells |
| title_full_unstemmed |
Expression of tumour-related Rac1b antagonizes B-Raf-induced senescence in colorectal cells |
| title_sort |
Expression of tumour-related Rac1b antagonizes B-Raf-induced senescence in colorectal cells |
| author |
Henriques, Andreia FA |
| author_facet |
Henriques, Andreia FA Barros, Patrícia Moyer, Mary Matos, Paulo Jordan, Peter |
| author_role |
author |
| author2 |
Barros, Patrícia Moyer, Mary Matos, Paulo Jordan, Peter |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
| dc.contributor.author.fl_str_mv |
Henriques, Andreia FA Barros, Patrícia Moyer, Mary Matos, Paulo Jordan, Peter |
| dc.subject.por.fl_str_mv |
Vias de Transdução de Sinal e Patologias Associadas Cancro Coloretal Rac1b Signalling Senescence B-Raf Colorectal Cancer Tumor Progression |
| topic |
Vias de Transdução de Sinal e Patologias Associadas Cancro Coloretal Rac1b Signalling Senescence B-Raf Colorectal Cancer Tumor Progression |
| description |
Erratum Cancer Lett. 2022 Dec 1;550:215936. doi: 10.1016/j.canlet.2022.215936. Epub 2022 Oct 11. |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015-12-28 2015-12-28T00:00:00Z 2019-01-01T01:30:10Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10400.18/3424 |
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http://hdl.handle.net/10400.18/3424 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Cancer Lett. 2015 Dec 28;369(2):368-75. doi: 10.1016/j.canlet.2015.08.027. Epub 2015 Sep 1 0304-3835 10.1016/j.canlet.2015.08.027 |
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application/pdf application/pdf |
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Elsevier |
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Elsevier |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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