In Vitro Cyto-and Genotoxicity Assessment of Antibacterial Paints with Triclosan and Isoborneol
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/10216/151489 |
Resumo: | Surfaces with antimicrobial properties are gaining notoriety as an efficient method to avoid surface contamination. Self-disinfecting paints are a promising strategy towards cleaner indoor environments by preventing the colonization of walls with microorganisms. However, its widespread use needs an appropriate toxicological safety evaluation due to the potential for biological disturbance associated to its biocidal activity. In this work, the cyto-and genotoxic assessment of two self-disinfecting paints containing the antimicrobial substances triclosan (TCS) and isoborneol (ISB) is performed. HaCaT and A549 cell lines models were selected for the in vitro assessment. To evaluate the cytotoxicity, tests by direct contact and on extracts obtained from leaching were performed following ISO 10993, whereas the genotoxicity was assessed by comet assay and cytokinesis-block micronucleus (CBMN) assay. The results showed low levels of cyto-and genotoxicity under the models and conditions tested, indicating that these substances have commercial potential. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
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In Vitro Cyto-and Genotoxicity Assessment of Antibacterial Paints with Triclosan and IsoborneolAntibacterial; Cytotoxicity; Genotoxicity; Isoborneol; Paint; TriclosanSurfaces with antimicrobial properties are gaining notoriety as an efficient method to avoid surface contamination. Self-disinfecting paints are a promising strategy towards cleaner indoor environments by preventing the colonization of walls with microorganisms. However, its widespread use needs an appropriate toxicological safety evaluation due to the potential for biological disturbance associated to its biocidal activity. In this work, the cyto-and genotoxic assessment of two self-disinfecting paints containing the antimicrobial substances triclosan (TCS) and isoborneol (ISB) is performed. HaCaT and A549 cell lines models were selected for the in vitro assessment. To evaluate the cytotoxicity, tests by direct contact and on extracts obtained from leaching were performed following ISO 10993, whereas the genotoxicity was assessed by comet assay and cytokinesis-block micronucleus (CBMN) assay. The results showed low levels of cyto-and genotoxicity under the models and conditions tested, indicating that these substances have commercial potential. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.MDPI20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/151489eng2305-630410.3390/toxics10020058Querido, MMRosário, FBessa, MJMendes, FTeixeira, JCTeixeira, JPPereira, CCinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-27T06:58:57Zoai:repositorio-aberto.up.pt:10216/151489Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-27T06:58:57Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
In Vitro Cyto-and Genotoxicity Assessment of Antibacterial Paints with Triclosan and Isoborneol |
title |
In Vitro Cyto-and Genotoxicity Assessment of Antibacterial Paints with Triclosan and Isoborneol |
spellingShingle |
In Vitro Cyto-and Genotoxicity Assessment of Antibacterial Paints with Triclosan and Isoborneol Querido, MM Antibacterial; Cytotoxicity; Genotoxicity; Isoborneol; Paint; Triclosan |
title_short |
In Vitro Cyto-and Genotoxicity Assessment of Antibacterial Paints with Triclosan and Isoborneol |
title_full |
In Vitro Cyto-and Genotoxicity Assessment of Antibacterial Paints with Triclosan and Isoborneol |
title_fullStr |
In Vitro Cyto-and Genotoxicity Assessment of Antibacterial Paints with Triclosan and Isoborneol |
title_full_unstemmed |
In Vitro Cyto-and Genotoxicity Assessment of Antibacterial Paints with Triclosan and Isoborneol |
title_sort |
In Vitro Cyto-and Genotoxicity Assessment of Antibacterial Paints with Triclosan and Isoborneol |
author |
Querido, MM |
author_facet |
Querido, MM Rosário, F Bessa, MJ Mendes, F Teixeira, JC Teixeira, JP Pereira, CC |
author_role |
author |
author2 |
Rosário, F Bessa, MJ Mendes, F Teixeira, JC Teixeira, JP Pereira, CC |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Querido, MM Rosário, F Bessa, MJ Mendes, F Teixeira, JC Teixeira, JP Pereira, CC |
dc.subject.por.fl_str_mv |
Antibacterial; Cytotoxicity; Genotoxicity; Isoborneol; Paint; Triclosan |
topic |
Antibacterial; Cytotoxicity; Genotoxicity; Isoborneol; Paint; Triclosan |
description |
Surfaces with antimicrobial properties are gaining notoriety as an efficient method to avoid surface contamination. Self-disinfecting paints are a promising strategy towards cleaner indoor environments by preventing the colonization of walls with microorganisms. However, its widespread use needs an appropriate toxicological safety evaluation due to the potential for biological disturbance associated to its biocidal activity. In this work, the cyto-and genotoxic assessment of two self-disinfecting paints containing the antimicrobial substances triclosan (TCS) and isoborneol (ISB) is performed. HaCaT and A549 cell lines models were selected for the in vitro assessment. To evaluate the cytotoxicity, tests by direct contact and on extracts obtained from leaching were performed following ISO 10993, whereas the genotoxicity was assessed by comet assay and cytokinesis-block micronucleus (CBMN) assay. The results showed low levels of cyto-and genotoxicity under the models and conditions tested, indicating that these substances have commercial potential. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2022-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/151489 |
url |
https://hdl.handle.net/10216/151489 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2305-6304 10.3390/toxics10020058 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817547427071131648 |