Determining the topology of peroxisomal proteins using protease protection assays

Detalhes bibliográficos
Autor(a) principal: Francisco, T
Data de Publicação: 2017
Outros Autores: Dias, AF, Pedrosa, AG, Grou, CP, Rodrigues, TA, Azevedo, JE
Tipo de documento: Livro
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://repositorio-aberto.up.pt/handle/10216/117911
Resumo: Protease protection assays are powerful tools to determine the topology of organelle proteins. Their simplicity, together with the fact that they are particularly suited to characterize endogenous proteins, are their major advantages and the reason why these assays have been in use for so many years. Here, we provide a detailed protocol to use with mammalian peroxisomes. Suggestions on how these assays can be controlled, and how to identify some technical pitfalls, are also presented.
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spelling Determining the topology of peroxisomal proteins using protease protection assaysProtease protection assays are powerful tools to determine the topology of organelle proteins. Their simplicity, together with the fact that they are particularly suited to characterize endogenous proteins, are their major advantages and the reason why these assays have been in use for so many years. Here, we provide a detailed protocol to use with mammalian peroxisomes. Suggestions on how these assays can be controlled, and how to identify some technical pitfalls, are also presented.Humana Press20172017-01-01T00:00:00Z2019-04-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookapplication/pdfhttps://repositorio-aberto.up.pt/handle/10216/117911eng1064-374510.1007/978-1-4939-6937-1_3Francisco, TDias, AFPedrosa, AGGrou, CPRodrigues, TAAzevedo, JEinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T12:27:17Zoai:repositorio-aberto.up.pt:10216/117911Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:20:38.390620Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Determining the topology of peroxisomal proteins using protease protection assays
title Determining the topology of peroxisomal proteins using protease protection assays
spellingShingle Determining the topology of peroxisomal proteins using protease protection assays
Francisco, T
title_short Determining the topology of peroxisomal proteins using protease protection assays
title_full Determining the topology of peroxisomal proteins using protease protection assays
title_fullStr Determining the topology of peroxisomal proteins using protease protection assays
title_full_unstemmed Determining the topology of peroxisomal proteins using protease protection assays
title_sort Determining the topology of peroxisomal proteins using protease protection assays
author Francisco, T
author_facet Francisco, T
Dias, AF
Pedrosa, AG
Grou, CP
Rodrigues, TA
Azevedo, JE
author_role author
author2 Dias, AF
Pedrosa, AG
Grou, CP
Rodrigues, TA
Azevedo, JE
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Francisco, T
Dias, AF
Pedrosa, AG
Grou, CP
Rodrigues, TA
Azevedo, JE
description Protease protection assays are powerful tools to determine the topology of organelle proteins. Their simplicity, together with the fact that they are particularly suited to characterize endogenous proteins, are their major advantages and the reason why these assays have been in use for so many years. Here, we provide a detailed protocol to use with mammalian peroxisomes. Suggestions on how these assays can be controlled, and how to identify some technical pitfalls, are also presented.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2019-04-14T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/book
format book
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio-aberto.up.pt/handle/10216/117911
url https://repositorio-aberto.up.pt/handle/10216/117911
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1064-3745
10.1007/978-1-4939-6937-1_3
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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