Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs

Detalhes bibliográficos
Autor(a) principal: Martinez, CA
Data de Publicação: 2019
Outros Autores: Cambra, JM, Parrilla, I, Roca, J, Ferreira-Dias, Graça, Pallares, FJ, Lucas, X, Vazquez, JM, Martinez, EA, Gil, MA
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/22150
Resumo: Research Areas: Veterinary Sciences
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spelling Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigsSeminal plasmaendometriumtranscriptomeembryopreimplantationpigResearch Areas: Veterinary SciencesABSTRACT - Background: Seminal plasma (SP) promotes sperm survival and fertilizing capacity, and potentially affects embryo development, presumably via specific signaling pathways to the internal female genital tract. Objectives: This study evaluated how heterologous SP, infused immediately before postcervical artificial insemination (AI) affected embryo development and the transcriptional pattern of the pig endometria containing embryos. Materials and Methods: Postweaning estrus sows (n = 34) received 40-mL intrauterine infusions of either heterologous pooled SP or Beltsville Thawing Solution (BTS; control) 30 min before AI of semen extended to 10% of homologous SR. Embryos (all sows) and endometrium samples (3 sows/group) were removed during laparotomy 6 days after the infusion of SP or BTS to morphologically evaluate the embryos to determine their developmental stage and to analyze the endometrial transcriptome using microarrays (PORGENE 1.0 ST GeneChip array, Affymetrix) followed by qPCR for further validation. Results: Embryo viability was equal between the groups (similar to 93%), but embryo development was significantly (P < 0.05) more advanced in the SP-treated group compared to control. A total of 1,604 endometrium transcripts were differentially expressed in the SP group compared to the control group. An enrichment analysis showed an overrepresentation of genes and pathways associated with the immune response, cytokine signaling, cell cycle, cell adhesion, and hormone response, among others. Conclusions: SP infusions prior to AI positively impacted the preimplantation embryo development and altered the expression of the endometrial genes and pathways potentially involved in embryo development.Arumugam Kumaresan, National Dairy Research Institute (ICAR), IndiaRepositório da Universidade de LisboaMartinez, CACambra, JMParrilla, IRoca, JFerreira-Dias, GraçaPallares, FJLucas, XVazquez, JMMartinez, EAGil, MA2021-10-06T15:49:54Z2019-12-182019-12-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22150engMartinez CA, Cambra JM, Parrilla I, Roca J, Ferreira-Dias G, Pallares FJ, Lucas X, Vazquez JM, Martinez EA, Gil MA. 2019. Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs. Frontiers in Veterinary Science. 6:465.10.3389/fvets.2019.004652297-1769info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:51:43Zoai:www.repository.utl.pt:10400.5/22150Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:38.317466Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs
title Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs
spellingShingle Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs
Martinez, CA
Seminal plasma
endometrium
transcriptome
embryo
preimplantation
pig
title_short Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs
title_full Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs
title_fullStr Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs
title_full_unstemmed Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs
title_sort Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs
author Martinez, CA
author_facet Martinez, CA
Cambra, JM
Parrilla, I
Roca, J
Ferreira-Dias, Graça
Pallares, FJ
Lucas, X
Vazquez, JM
Martinez, EA
Gil, MA
author_role author
author2 Cambra, JM
Parrilla, I
Roca, J
Ferreira-Dias, Graça
Pallares, FJ
Lucas, X
Vazquez, JM
Martinez, EA
Gil, MA
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Martinez, CA
Cambra, JM
Parrilla, I
Roca, J
Ferreira-Dias, Graça
Pallares, FJ
Lucas, X
Vazquez, JM
Martinez, EA
Gil, MA
dc.subject.por.fl_str_mv Seminal plasma
endometrium
transcriptome
embryo
preimplantation
pig
topic Seminal plasma
endometrium
transcriptome
embryo
preimplantation
pig
description Research Areas: Veterinary Sciences
publishDate 2019
dc.date.none.fl_str_mv 2019-12-18
2019-12-18T00:00:00Z
2021-10-06T15:49:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/22150
url http://hdl.handle.net/10400.5/22150
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Martinez CA, Cambra JM, Parrilla I, Roca J, Ferreira-Dias G, Pallares FJ, Lucas X, Vazquez JM, Martinez EA, Gil MA. 2019. Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs. Frontiers in Veterinary Science. 6:465.
10.3389/fvets.2019.00465
2297-1769
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Arumugam Kumaresan, National Dairy Research Institute (ICAR), India
publisher.none.fl_str_mv Arumugam Kumaresan, National Dairy Research Institute (ICAR), India
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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