Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/22150 |
Resumo: | Research Areas: Veterinary Sciences |
id |
RCAP_641a73ec897bd25b9411954e56309aca |
---|---|
oai_identifier_str |
oai:www.repository.utl.pt:10400.5/22150 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigsSeminal plasmaendometriumtranscriptomeembryopreimplantationpigResearch Areas: Veterinary SciencesABSTRACT - Background: Seminal plasma (SP) promotes sperm survival and fertilizing capacity, and potentially affects embryo development, presumably via specific signaling pathways to the internal female genital tract. Objectives: This study evaluated how heterologous SP, infused immediately before postcervical artificial insemination (AI) affected embryo development and the transcriptional pattern of the pig endometria containing embryos. Materials and Methods: Postweaning estrus sows (n = 34) received 40-mL intrauterine infusions of either heterologous pooled SP or Beltsville Thawing Solution (BTS; control) 30 min before AI of semen extended to 10% of homologous SR. Embryos (all sows) and endometrium samples (3 sows/group) were removed during laparotomy 6 days after the infusion of SP or BTS to morphologically evaluate the embryos to determine their developmental stage and to analyze the endometrial transcriptome using microarrays (PORGENE 1.0 ST GeneChip array, Affymetrix) followed by qPCR for further validation. Results: Embryo viability was equal between the groups (similar to 93%), but embryo development was significantly (P < 0.05) more advanced in the SP-treated group compared to control. A total of 1,604 endometrium transcripts were differentially expressed in the SP group compared to the control group. An enrichment analysis showed an overrepresentation of genes and pathways associated with the immune response, cytokine signaling, cell cycle, cell adhesion, and hormone response, among others. Conclusions: SP infusions prior to AI positively impacted the preimplantation embryo development and altered the expression of the endometrial genes and pathways potentially involved in embryo development.Arumugam Kumaresan, National Dairy Research Institute (ICAR), IndiaRepositório da Universidade de LisboaMartinez, CACambra, JMParrilla, IRoca, JFerreira-Dias, GraçaPallares, FJLucas, XVazquez, JMMartinez, EAGil, MA2021-10-06T15:49:54Z2019-12-182019-12-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22150engMartinez CA, Cambra JM, Parrilla I, Roca J, Ferreira-Dias G, Pallares FJ, Lucas X, Vazquez JM, Martinez EA, Gil MA. 2019. Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs. Frontiers in Veterinary Science. 6:465.10.3389/fvets.2019.004652297-1769info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:51:43Zoai:www.repository.utl.pt:10400.5/22150Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:38.317466Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs |
title |
Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs |
spellingShingle |
Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs Martinez, CA Seminal plasma endometrium transcriptome embryo preimplantation pig |
title_short |
Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs |
title_full |
Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs |
title_fullStr |
Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs |
title_full_unstemmed |
Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs |
title_sort |
Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs |
author |
Martinez, CA |
author_facet |
Martinez, CA Cambra, JM Parrilla, I Roca, J Ferreira-Dias, Graça Pallares, FJ Lucas, X Vazquez, JM Martinez, EA Gil, MA |
author_role |
author |
author2 |
Cambra, JM Parrilla, I Roca, J Ferreira-Dias, Graça Pallares, FJ Lucas, X Vazquez, JM Martinez, EA Gil, MA |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Martinez, CA Cambra, JM Parrilla, I Roca, J Ferreira-Dias, Graça Pallares, FJ Lucas, X Vazquez, JM Martinez, EA Gil, MA |
dc.subject.por.fl_str_mv |
Seminal plasma endometrium transcriptome embryo preimplantation pig |
topic |
Seminal plasma endometrium transcriptome embryo preimplantation pig |
description |
Research Areas: Veterinary Sciences |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-18 2019-12-18T00:00:00Z 2021-10-06T15:49:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/22150 |
url |
http://hdl.handle.net/10400.5/22150 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Martinez CA, Cambra JM, Parrilla I, Roca J, Ferreira-Dias G, Pallares FJ, Lucas X, Vazquez JM, Martinez EA, Gil MA. 2019. Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs. Frontiers in Veterinary Science. 6:465. 10.3389/fvets.2019.00465 2297-1769 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Arumugam Kumaresan, National Dairy Research Institute (ICAR), India |
publisher.none.fl_str_mv |
Arumugam Kumaresan, National Dairy Research Institute (ICAR), India |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131159484956672 |