Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices

Detalhes bibliográficos
Autor(a) principal: Maduro, Filipa Cristiana Duarte Fangueiro
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/785
Resumo: Dissertação mest., Biotecnologia, Universidade do Algarve, 2009
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spelling Penicillin G acylase encapsulation studies in polyvinyl alcohol based matricesTesesPenicilinaAntibióticosDissertação mest., Biotecnologia, Universidade do Algarve, 2009Penicillin G acylase (EC 3.5.1.11) (PGA) hydrolyses penicillin G (PG) producing 6-aminopenicillanic acid (6-APA), an important building block in the synthesis of semi-synthetic antibiotics such as ampicillin and amoxicillin. The synthesis of these antibiotics by PGA is already successfully used in lab and pilot scales and more recently in the synthesis of cefalexin (CEX) at industrial scale. This work characterizes the stability and activity of PGA when immobilized in a polyvinyl alcohol based matrix (Lentikat® liquid), PVA/LL by a new and simple entrapment strategy. The biocatalyst retains from 22 to 66% of its original activity, depending on enzyme load. Hydrolysis and synthesis reactions were tested and the immobilized enzyme can be reused for, at least, 10 consecutive batches without decay of initial activity. The preferential pH and temperature for the hydrolysis of PG are 8 and 37ºC, respectively, it is stable at 4ºC and its half-life, at 25ºC and pH 8 is roughly 8 days. For the synthesis of CEX there was no decay of initial activity for at least 50 hours, at 14ºC and pH 7.2, which indicates a high operational stability. Substrate inhibition by 7-aminodesacetoxicefalosporanic acid (7-ADCA) was observed both for the free and the immobilized enzyme. These forms of enzyme presented synthesis of CEX and hydrolysis of PGM ratio (S/H) of 4.2 and 2.5, respectively. The biocatalyst (PGA_PVA beads) is suitable for the production of cefalexin in industry since it showed a high operational stability, without enzyme leakage during the reaction and between washing steps.Fonseca, Luís Pina daPower, DeborahSapientiaMaduro, Filipa Cristiana Duarte Fangueiro2011-09-07T16:05:34Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.1/785eng577 MAD*Pen Caveinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:11:37Zoai:sapientia.ualg.pt:10400.1/785Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:55:05.150026Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices
title Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices
spellingShingle Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices
Maduro, Filipa Cristiana Duarte Fangueiro
Teses
Penicilina
Antibióticos
title_short Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices
title_full Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices
title_fullStr Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices
title_full_unstemmed Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices
title_sort Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices
author Maduro, Filipa Cristiana Duarte Fangueiro
author_facet Maduro, Filipa Cristiana Duarte Fangueiro
author_role author
dc.contributor.none.fl_str_mv Fonseca, Luís Pina da
Power, Deborah
Sapientia
dc.contributor.author.fl_str_mv Maduro, Filipa Cristiana Duarte Fangueiro
dc.subject.por.fl_str_mv Teses
Penicilina
Antibióticos
topic Teses
Penicilina
Antibióticos
description Dissertação mest., Biotecnologia, Universidade do Algarve, 2009
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01T00:00:00Z
2011-09-07T16:05:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/785
url http://hdl.handle.net/10400.1/785
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 577 MAD*Pen Cave
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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