Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/785 |
Resumo: | Dissertação mest., Biotecnologia, Universidade do Algarve, 2009 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Penicillin G acylase encapsulation studies in polyvinyl alcohol based matricesTesesPenicilinaAntibióticosDissertação mest., Biotecnologia, Universidade do Algarve, 2009Penicillin G acylase (EC 3.5.1.11) (PGA) hydrolyses penicillin G (PG) producing 6-aminopenicillanic acid (6-APA), an important building block in the synthesis of semi-synthetic antibiotics such as ampicillin and amoxicillin. The synthesis of these antibiotics by PGA is already successfully used in lab and pilot scales and more recently in the synthesis of cefalexin (CEX) at industrial scale. This work characterizes the stability and activity of PGA when immobilized in a polyvinyl alcohol based matrix (Lentikat® liquid), PVA/LL by a new and simple entrapment strategy. The biocatalyst retains from 22 to 66% of its original activity, depending on enzyme load. Hydrolysis and synthesis reactions were tested and the immobilized enzyme can be reused for, at least, 10 consecutive batches without decay of initial activity. The preferential pH and temperature for the hydrolysis of PG are 8 and 37ºC, respectively, it is stable at 4ºC and its half-life, at 25ºC and pH 8 is roughly 8 days. For the synthesis of CEX there was no decay of initial activity for at least 50 hours, at 14ºC and pH 7.2, which indicates a high operational stability. Substrate inhibition by 7-aminodesacetoxicefalosporanic acid (7-ADCA) was observed both for the free and the immobilized enzyme. These forms of enzyme presented synthesis of CEX and hydrolysis of PGM ratio (S/H) of 4.2 and 2.5, respectively. The biocatalyst (PGA_PVA beads) is suitable for the production of cefalexin in industry since it showed a high operational stability, without enzyme leakage during the reaction and between washing steps.Fonseca, Luís Pina daPower, DeborahSapientiaMaduro, Filipa Cristiana Duarte Fangueiro2011-09-07T16:05:34Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.1/785eng577 MAD*Pen Caveinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:11:37Zoai:sapientia.ualg.pt:10400.1/785Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:55:05.150026Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices |
title |
Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices |
spellingShingle |
Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices Maduro, Filipa Cristiana Duarte Fangueiro Teses Penicilina Antibióticos |
title_short |
Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices |
title_full |
Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices |
title_fullStr |
Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices |
title_full_unstemmed |
Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices |
title_sort |
Penicillin G acylase encapsulation studies in polyvinyl alcohol based matrices |
author |
Maduro, Filipa Cristiana Duarte Fangueiro |
author_facet |
Maduro, Filipa Cristiana Duarte Fangueiro |
author_role |
author |
dc.contributor.none.fl_str_mv |
Fonseca, Luís Pina da Power, Deborah Sapientia |
dc.contributor.author.fl_str_mv |
Maduro, Filipa Cristiana Duarte Fangueiro |
dc.subject.por.fl_str_mv |
Teses Penicilina Antibióticos |
topic |
Teses Penicilina Antibióticos |
description |
Dissertação mest., Biotecnologia, Universidade do Algarve, 2009 |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2009-01-01T00:00:00Z 2011-09-07T16:05:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/785 |
url |
http://hdl.handle.net/10400.1/785 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
577 MAD*Pen Cave |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799133151462686720 |