Redox–Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/7651 |
Resumo: | This article belongs to the Special Issue Peroxiredoxin. |
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Redox–Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure TherapyCys-sulfinylation/Cys-sulfonylationGlyceraldehyde-3-phosphate Dehydrogenase (GAPDH)Obstructive Sleep Apnea (OSA)Peroxiredoxin-2 (PRDX2)Positive Airway Pressure (PAP)Proteomics-biomarkersGenómica Funcional e EstruturalThis article belongs to the Special Issue Peroxiredoxin.In this study, we examined the effect of six months of positive airway pressure (PAP) therapy on Obstructive Sleep Apnea (OSA) red blood cell (RBC) proteome by two dimensional difference gel electrophoresis (2D-DIGE) - based proteomics followed by Western blotting (WB) validation. The discovered dysregulated proteins/proteoforms are associated with cell death, H2O2 catabolic/metabolic process, stress response, and protein oligomerization. Validation by nonreducing WB was performed for peroxiredoxin-2 (PRDX2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by using antibodies against the sulfinylated/sulfonylated cysteine of these proteins to better evaluate their redox-oligomeric states under OSA and/or in response to PAP therapy. The results indicated that the redox-oligomeric state of GAPDH and PRDX2 involving overoxidation by sulfinic/sulfonic acids were differentially modulated in OSA RBC, which might be compromising RBC homeostasis. PAP therapy by restoring this modulation induced a higher oligomerization of overoxidized GAPDH and PRDX2 in some patients that could be associated with eryptosis and the chaperone "gain" of function, respectively. This varied response following PAP may result from the complex interplay between OSA and OSA metabolic comorbidity. Hence, information on the redox status of PRDX2 and GAPDH in RBC will help to better recognize OSA subtypes and predict the therapeutic response in these patients. GAPDH monomer combined with body mass index (BMI) and PRDX2 S-S dimer combined with homeostatic model assessment for insulin resistance (HOMA-IR) showed to be very promising biomarkers to predict OSA and OSA severity, respectively.Project partially supported by Harvard Medical School-Portugal Program (HMSPICJ/0022/2011), ToxOmics—Centre for Toxicogenomics and Human Health (FCT-UID/BIM/00009/2013), Fundação para a Ciência e a Tecnologia (FCT)/Poly-Annual Funding Program and FEDER/Saúde XXI Program, Portugal, RNEM- National Mass Spectrometry Networking—FCT Strategic Infrastructure and PhD fellowship, FCT-SFRH/BD/133511/2017, Portugal.MDPIRepositório Científico do Instituto Nacional de SaúdeValentim-Coelho, CristinaVaz, FátimaAntunes, MaríliaNeves, SofiaMartins, Inês L.Osório, HugoFeliciano, AméliaPinto, PaulaBárbara, CristinaPenque, Deborah2021-04-07T15:02:06Z2020-11-262020-11-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7651engAntioxidants (Basel). 2020 Nov 26;9(12):1184. doi: 10.3390/antiox9121184.2076-392110.3390/antiox9121184info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:08Zoai:repositorio.insa.pt:10400.18/7651Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:17.663947Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Redox–Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy |
title |
Redox–Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy |
spellingShingle |
Redox–Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy Valentim-Coelho, Cristina Cys-sulfinylation/Cys-sulfonylation Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Obstructive Sleep Apnea (OSA) Peroxiredoxin-2 (PRDX2) Positive Airway Pressure (PAP) Proteomics-biomarkers Genómica Funcional e Estrutural |
title_short |
Redox–Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy |
title_full |
Redox–Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy |
title_fullStr |
Redox–Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy |
title_full_unstemmed |
Redox–Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy |
title_sort |
Redox–Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy |
author |
Valentim-Coelho, Cristina |
author_facet |
Valentim-Coelho, Cristina Vaz, Fátima Antunes, Marília Neves, Sofia Martins, Inês L. Osório, Hugo Feliciano, Amélia Pinto, Paula Bárbara, Cristina Penque, Deborah |
author_role |
author |
author2 |
Vaz, Fátima Antunes, Marília Neves, Sofia Martins, Inês L. Osório, Hugo Feliciano, Amélia Pinto, Paula Bárbara, Cristina Penque, Deborah |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Valentim-Coelho, Cristina Vaz, Fátima Antunes, Marília Neves, Sofia Martins, Inês L. Osório, Hugo Feliciano, Amélia Pinto, Paula Bárbara, Cristina Penque, Deborah |
dc.subject.por.fl_str_mv |
Cys-sulfinylation/Cys-sulfonylation Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Obstructive Sleep Apnea (OSA) Peroxiredoxin-2 (PRDX2) Positive Airway Pressure (PAP) Proteomics-biomarkers Genómica Funcional e Estrutural |
topic |
Cys-sulfinylation/Cys-sulfonylation Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Obstructive Sleep Apnea (OSA) Peroxiredoxin-2 (PRDX2) Positive Airway Pressure (PAP) Proteomics-biomarkers Genómica Funcional e Estrutural |
description |
This article belongs to the Special Issue Peroxiredoxin. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-26 2020-11-26T00:00:00Z 2021-04-07T15:02:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/7651 |
url |
http://hdl.handle.net/10400.18/7651 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Antioxidants (Basel). 2020 Nov 26;9(12):1184. doi: 10.3390/antiox9121184. 2076-3921 10.3390/antiox9121184 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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