Redox–oligomeric state of Peroxiredoxin-2 and Glyceraldehyde-3-phosphate dehydrogenase in obstructive sleep apnea red blood cells under positive airway pressure therapy

Detalhes bibliográficos
Autor(a) principal: Valentim Coelho, Cristina
Data de Publicação: 2020
Outros Autores: Vaz, Fátima, Antunes, Marilia, Neves, Sofia, Martins, Inês L., Osório, Hugo, Feliciano, Amélia, Pinto, Paula, Bárbara, Cristina, Penque, Deborah
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/45242
Resumo: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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spelling Redox–oligomeric state of Peroxiredoxin-2 and Glyceraldehyde-3-phosphate dehydrogenase in obstructive sleep apnea red blood cells under positive airway pressure therapyObstructive sleep apnea (OSA)Positive airway pressure (PAP)Peroxiredoxin-2 (PRDX2)Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)Cys-sulfinylation/Cys-sulfonylationProteomics-biomarkers© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).In this study, we examined the effect of six months of positive airway pressure (PAP) therapy on Obstructive Sleep Apnea (OSA) red blood cell (RBC) proteome by two dimensional difference gel electrophoresis (2D-DIGE) - based proteomics followed by Western blotting (WB) validation. The discovered dysregulated proteins/proteoforms are associated with cell death, H2O2 catabolic/metabolic process, stress response, and protein oligomerization. Validation by nonreducing WB was performed for peroxiredoxin-2 (PRDX2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by using antibodies against the sulfinylated/sulfonylated cysteine of these proteins to better evaluate their redox-oligomeric states under OSA and/or in response to PAP therapy. The results indicated that the redox-oligomeric state of GAPDH and PRDX2 involving overoxidation by sulfinic/sulfonic acids were differentially modulated in OSA RBC, which might be compromising RBC homeostasis. PAP therapy by restoring this modulation induced a higher oligomerization of overoxidized GAPDH and PRDX2 in some patients that could be associated with eryptosis and the chaperone "gain" of function, respectively. This varied response following PAP may result from the complex interplay between OSA and OSA metabolic comorbidity. Hence, information on the redox status of PRDX2 and GAPDH in RBC will help to better recognize OSA subtypes and predict the therapeutic response in these patients. GAPDH monomer combined with body mass index (BMI) and PRDX2 S-S dimer combined with homeostatic model assessment for insulin resistance (HOMA-IR) showed to be very promising biomarkers to predict OSA and OSA severity, respectively.Project partially supported by Harvard Medical School-Portugal Program (HMSPICJ/0022/2011), ToxOmics - Centre for Toxicogenomics and Human Health (FCT-UID/BIM/00009/2013), Fundação para a Ciência e a Tecnologia (FCT)/Poly-Annual Funding Program and FEDER/Saúde XXI Program, Portugal, RNEM- National Mass Spectrometry Networking—FCT Strategic Infrastructure and PhD fellowship, FCT-SFRH/BD/133511/2017, Portugal.MDPIRepositório da Universidade de LisboaValentim Coelho, CristinaVaz, FátimaAntunes, MariliaNeves, SofiaMartins, Inês L.Osório, HugoFeliciano, AméliaPinto, PaulaBárbara, CristinaPenque, Deborah2020-12-10T16:38:08Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/45242engAntioxidants 2020, 9, 1184.2076-392110.3390/antiox9121184info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:46:56Zoai:repositorio.ul.pt:10451/45242Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:57:44.471437Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Redox–oligomeric state of Peroxiredoxin-2 and Glyceraldehyde-3-phosphate dehydrogenase in obstructive sleep apnea red blood cells under positive airway pressure therapy
title Redox–oligomeric state of Peroxiredoxin-2 and Glyceraldehyde-3-phosphate dehydrogenase in obstructive sleep apnea red blood cells under positive airway pressure therapy
spellingShingle Redox–oligomeric state of Peroxiredoxin-2 and Glyceraldehyde-3-phosphate dehydrogenase in obstructive sleep apnea red blood cells under positive airway pressure therapy
Valentim Coelho, Cristina
Obstructive sleep apnea (OSA)
Positive airway pressure (PAP)
Peroxiredoxin-2 (PRDX2)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Cys-sulfinylation/Cys-sulfonylation
Proteomics-biomarkers
title_short Redox–oligomeric state of Peroxiredoxin-2 and Glyceraldehyde-3-phosphate dehydrogenase in obstructive sleep apnea red blood cells under positive airway pressure therapy
title_full Redox–oligomeric state of Peroxiredoxin-2 and Glyceraldehyde-3-phosphate dehydrogenase in obstructive sleep apnea red blood cells under positive airway pressure therapy
title_fullStr Redox–oligomeric state of Peroxiredoxin-2 and Glyceraldehyde-3-phosphate dehydrogenase in obstructive sleep apnea red blood cells under positive airway pressure therapy
title_full_unstemmed Redox–oligomeric state of Peroxiredoxin-2 and Glyceraldehyde-3-phosphate dehydrogenase in obstructive sleep apnea red blood cells under positive airway pressure therapy
title_sort Redox–oligomeric state of Peroxiredoxin-2 and Glyceraldehyde-3-phosphate dehydrogenase in obstructive sleep apnea red blood cells under positive airway pressure therapy
author Valentim Coelho, Cristina
author_facet Valentim Coelho, Cristina
Vaz, Fátima
Antunes, Marilia
Neves, Sofia
Martins, Inês L.
Osório, Hugo
Feliciano, Amélia
Pinto, Paula
Bárbara, Cristina
Penque, Deborah
author_role author
author2 Vaz, Fátima
Antunes, Marilia
Neves, Sofia
Martins, Inês L.
Osório, Hugo
Feliciano, Amélia
Pinto, Paula
Bárbara, Cristina
Penque, Deborah
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Valentim Coelho, Cristina
Vaz, Fátima
Antunes, Marilia
Neves, Sofia
Martins, Inês L.
Osório, Hugo
Feliciano, Amélia
Pinto, Paula
Bárbara, Cristina
Penque, Deborah
dc.subject.por.fl_str_mv Obstructive sleep apnea (OSA)
Positive airway pressure (PAP)
Peroxiredoxin-2 (PRDX2)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Cys-sulfinylation/Cys-sulfonylation
Proteomics-biomarkers
topic Obstructive sleep apnea (OSA)
Positive airway pressure (PAP)
Peroxiredoxin-2 (PRDX2)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Cys-sulfinylation/Cys-sulfonylation
Proteomics-biomarkers
description © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
publishDate 2020
dc.date.none.fl_str_mv 2020-12-10T16:38:08Z
2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/45242
url http://hdl.handle.net/10451/45242
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Antioxidants 2020, 9, 1184.
2076-3921
10.3390/antiox9121184
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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