Presence of pyrimethamine and cycloguanil resistance genotype in West Africa? Evidence of a single mutation 59Arg in the dhfr gene of Plasmodium falciparum.

Detalhes bibliográficos
Autor(a) principal: Pinheiro, L
Data de Publicação: 2003
Outros Autores: Rosário, V E
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/1190
Resumo: In 3 different geographical areas of West Africa (Guinea-Bissau, S. Tomé e Princípe and Angola) where chloroquine activity against P. falciparum seems to have decreased, the choice for prevention and/or treatment of malaria is often based on second line drugs such as Fansidar® (pyrimethamine/sulphadoxine). Little is known about the genetic basis of dhfr-ts gene mutations from parasites from these areas. In this study a PCR/RFLP methodology was used to screen 33 field isolates, without intervening steps of in vitro culture, for the pyrimethamine and cycloguanil sensitive/resistant genotype of P. falciparum. Analysis of the digested fragments revealed a prevalence of a Ser108 to Asn108 change in 63.63% of the samples tested.
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spelling Presence of pyrimethamine and cycloguanil resistance genotype in West Africa? Evidence of a single mutation 59Arg in the dhfr gene of Plasmodium falciparum.Resistência à pirimetamina e ao cicloguanil na áfrica occidental presença da mutação 59Arg no gene dhfr do Plasmodium falciparum.In 3 different geographical areas of West Africa (Guinea-Bissau, S. Tomé e Princípe and Angola) where chloroquine activity against P. falciparum seems to have decreased, the choice for prevention and/or treatment of malaria is often based on second line drugs such as Fansidar® (pyrimethamine/sulphadoxine). Little is known about the genetic basis of dhfr-ts gene mutations from parasites from these areas. In this study a PCR/RFLP methodology was used to screen 33 field isolates, without intervening steps of in vitro culture, for the pyrimethamine and cycloguanil sensitive/resistant genotype of P. falciparum. Analysis of the digested fragments revealed a prevalence of a Ser108 to Asn108 change in 63.63% of the samples tested.In 3 different geographical areas of West Africa (Guinea-Bissau, S. Tomé e Princípe and Angola) where chloroquine activity against P. falciparum seems to have decreased, the choice for prevention and/or treatment of malaria is often based on second line drugs such as Fansidar® (pyrimethamine/sulphadoxine). Little is known about the genetic basis of dhfr-ts gene mutations from parasites from these areas. In this study a PCR/RFLP methodology was used to screen 33 field isolates, without intervening steps of in vitro culture, for the pyrimethamine and cycloguanil sensitive/resistant genotype of P. falciparum. Analysis of the digested fragments revealed a prevalence of a Ser108 to Asn108 change in 63.63% of the samples tested.Ordem dos Médicos2003-08-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/1190oai:ojs.www.actamedicaportuguesa.com:article/1190Acta Médica Portuguesa; Vol. 16 No. 4 (2003): July-August; 235-8Acta Médica Portuguesa; Vol. 16 N.º 4 (2003): Julho-Agosto; 235-81646-07580870-399Xreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPporhttps://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/1190https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/1190/842Pinheiro, LRosário, V Einfo:eu-repo/semantics/openAccess2022-12-20T10:57:36Zoai:ojs.www.actamedicaportuguesa.com:article/1190Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:17:01.232161Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Presence of pyrimethamine and cycloguanil resistance genotype in West Africa? Evidence of a single mutation 59Arg in the dhfr gene of Plasmodium falciparum.
Resistência à pirimetamina e ao cicloguanil na áfrica occidental presença da mutação 59Arg no gene dhfr do Plasmodium falciparum.
title Presence of pyrimethamine and cycloguanil resistance genotype in West Africa? Evidence of a single mutation 59Arg in the dhfr gene of Plasmodium falciparum.
spellingShingle Presence of pyrimethamine and cycloguanil resistance genotype in West Africa? Evidence of a single mutation 59Arg in the dhfr gene of Plasmodium falciparum.
Pinheiro, L
title_short Presence of pyrimethamine and cycloguanil resistance genotype in West Africa? Evidence of a single mutation 59Arg in the dhfr gene of Plasmodium falciparum.
title_full Presence of pyrimethamine and cycloguanil resistance genotype in West Africa? Evidence of a single mutation 59Arg in the dhfr gene of Plasmodium falciparum.
title_fullStr Presence of pyrimethamine and cycloguanil resistance genotype in West Africa? Evidence of a single mutation 59Arg in the dhfr gene of Plasmodium falciparum.
title_full_unstemmed Presence of pyrimethamine and cycloguanil resistance genotype in West Africa? Evidence of a single mutation 59Arg in the dhfr gene of Plasmodium falciparum.
title_sort Presence of pyrimethamine and cycloguanil resistance genotype in West Africa? Evidence of a single mutation 59Arg in the dhfr gene of Plasmodium falciparum.
author Pinheiro, L
author_facet Pinheiro, L
Rosário, V E
author_role author
author2 Rosário, V E
author2_role author
dc.contributor.author.fl_str_mv Pinheiro, L
Rosário, V E
description In 3 different geographical areas of West Africa (Guinea-Bissau, S. Tomé e Princípe and Angola) where chloroquine activity against P. falciparum seems to have decreased, the choice for prevention and/or treatment of malaria is often based on second line drugs such as Fansidar® (pyrimethamine/sulphadoxine). Little is known about the genetic basis of dhfr-ts gene mutations from parasites from these areas. In this study a PCR/RFLP methodology was used to screen 33 field isolates, without intervening steps of in vitro culture, for the pyrimethamine and cycloguanil sensitive/resistant genotype of P. falciparum. Analysis of the digested fragments revealed a prevalence of a Ser108 to Asn108 change in 63.63% of the samples tested.
publishDate 2003
dc.date.none.fl_str_mv 2003-08-31
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/1190
oai:ojs.www.actamedicaportuguesa.com:article/1190
url https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/1190
identifier_str_mv oai:ojs.www.actamedicaportuguesa.com:article/1190
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/1190
https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/1190/842
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Ordem dos Médicos
publisher.none.fl_str_mv Ordem dos Médicos
dc.source.none.fl_str_mv Acta Médica Portuguesa; Vol. 16 No. 4 (2003): July-August; 235-8
Acta Médica Portuguesa; Vol. 16 N.º 4 (2003): Julho-Agosto; 235-8
1646-0758
0870-399X
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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