Membrane fractionation of Cynara cardunculus swine blood hydrolysate: ingredients of high nutritional and nutraceutical value
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/38115 |
Resumo: | This work proposes an innovative approach to valorise swine blood based on enzymatic hydrolysis and membrane fractionations. Hydrolysis with Cynara cardunculus enzymes, followed by microfiltration and double nanofiltration generated three high protein fractions, retentate of microfiltration (RMF; >0.5 µm) and retentate of nanofiltration (RNF; >3 kDa) with approximately 90% of protein on a dry basis and filtrate of nanofiltrate (FNF; <3 kDa) with 65%. FNF, rich in low molecular weight peptides, showed excellent antioxidants (ABTS and ORAC of 911.81 and 532.82 µmol TE g−1 DB, respectively) and antihypertensive (IC50 of 28.51 µg mL−1) potential. By peptidomics and in silico analysis, 43 unique sequences of interest were found, among which LVV-Hemorphin-7 was identified. This hemorphin was demonstrated as the main responsible for the observed bioactivity. Complementary results showed a prebiotic effect mainly for the growth of Bifidobacterium animalis Bo, as well as interesting free amino acids (mainly glutamic acid, leucine, alanine, phenylalanine and aspartic acid) and mineral (e.g., Ca, Mg, P, K and Na) profiles. No antibacterial effect was verified for the seven pathogenic bacteria tested. This study allowed obtaining new ingredient of high nutritional and nutraceutical value for human consumption, with a perspective of sustainability and industrial viability. |
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Membrane fractionation of Cynara cardunculus swine blood hydrolysate: ingredients of high nutritional and nutraceutical valueACE inhibitorAntioxidant activityBioactive peptidesBIOPEPEnzymatic hydrolysisFunctional ingredientSwine bloodWaste valorisationThis work proposes an innovative approach to valorise swine blood based on enzymatic hydrolysis and membrane fractionations. Hydrolysis with Cynara cardunculus enzymes, followed by microfiltration and double nanofiltration generated three high protein fractions, retentate of microfiltration (RMF; >0.5 µm) and retentate of nanofiltration (RNF; >3 kDa) with approximately 90% of protein on a dry basis and filtrate of nanofiltrate (FNF; <3 kDa) with 65%. FNF, rich in low molecular weight peptides, showed excellent antioxidants (ABTS and ORAC of 911.81 and 532.82 µmol TE g−1 DB, respectively) and antihypertensive (IC50 of 28.51 µg mL−1) potential. By peptidomics and in silico analysis, 43 unique sequences of interest were found, among which LVV-Hemorphin-7 was identified. This hemorphin was demonstrated as the main responsible for the observed bioactivity. Complementary results showed a prebiotic effect mainly for the growth of Bifidobacterium animalis Bo, as well as interesting free amino acids (mainly glutamic acid, leucine, alanine, phenylalanine and aspartic acid) and mineral (e.g., Ca, Mg, P, K and Na) profiles. No antibacterial effect was verified for the seven pathogenic bacteria tested. This study allowed obtaining new ingredient of high nutritional and nutraceutical value for human consumption, with a perspective of sustainability and industrial viability.Veritati - Repositório Institucional da Universidade Católica PortuguesaAraújo-Rodrigues, HelenaCoscueta, Ezequiel R.Pereira, Miguel F.Cunha, Sara A.Almeida, AndréRosa, AnaMartins, RuiPereira, Carlos D.Pintado, Manuela E.2022-082022-08-01T00:00:00Z2023-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/38115eng0963-996910.1016/j.foodres.2022.1115498513290795935840243000826360000001info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:43:36Zoai:repositorio.ucp.pt:10400.14/38115Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:31:04.005636Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Membrane fractionation of Cynara cardunculus swine blood hydrolysate: ingredients of high nutritional and nutraceutical value |
title |
Membrane fractionation of Cynara cardunculus swine blood hydrolysate: ingredients of high nutritional and nutraceutical value |
spellingShingle |
Membrane fractionation of Cynara cardunculus swine blood hydrolysate: ingredients of high nutritional and nutraceutical value Araújo-Rodrigues, Helena ACE inhibitor Antioxidant activity Bioactive peptides BIOPEP Enzymatic hydrolysis Functional ingredient Swine blood Waste valorisation |
title_short |
Membrane fractionation of Cynara cardunculus swine blood hydrolysate: ingredients of high nutritional and nutraceutical value |
title_full |
Membrane fractionation of Cynara cardunculus swine blood hydrolysate: ingredients of high nutritional and nutraceutical value |
title_fullStr |
Membrane fractionation of Cynara cardunculus swine blood hydrolysate: ingredients of high nutritional and nutraceutical value |
title_full_unstemmed |
Membrane fractionation of Cynara cardunculus swine blood hydrolysate: ingredients of high nutritional and nutraceutical value |
title_sort |
Membrane fractionation of Cynara cardunculus swine blood hydrolysate: ingredients of high nutritional and nutraceutical value |
author |
Araújo-Rodrigues, Helena |
author_facet |
Araújo-Rodrigues, Helena Coscueta, Ezequiel R. Pereira, Miguel F. Cunha, Sara A. Almeida, André Rosa, Ana Martins, Rui Pereira, Carlos D. Pintado, Manuela E. |
author_role |
author |
author2 |
Coscueta, Ezequiel R. Pereira, Miguel F. Cunha, Sara A. Almeida, André Rosa, Ana Martins, Rui Pereira, Carlos D. Pintado, Manuela E. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Araújo-Rodrigues, Helena Coscueta, Ezequiel R. Pereira, Miguel F. Cunha, Sara A. Almeida, André Rosa, Ana Martins, Rui Pereira, Carlos D. Pintado, Manuela E. |
dc.subject.por.fl_str_mv |
ACE inhibitor Antioxidant activity Bioactive peptides BIOPEP Enzymatic hydrolysis Functional ingredient Swine blood Waste valorisation |
topic |
ACE inhibitor Antioxidant activity Bioactive peptides BIOPEP Enzymatic hydrolysis Functional ingredient Swine blood Waste valorisation |
description |
This work proposes an innovative approach to valorise swine blood based on enzymatic hydrolysis and membrane fractionations. Hydrolysis with Cynara cardunculus enzymes, followed by microfiltration and double nanofiltration generated three high protein fractions, retentate of microfiltration (RMF; >0.5 µm) and retentate of nanofiltration (RNF; >3 kDa) with approximately 90% of protein on a dry basis and filtrate of nanofiltrate (FNF; <3 kDa) with 65%. FNF, rich in low molecular weight peptides, showed excellent antioxidants (ABTS and ORAC of 911.81 and 532.82 µmol TE g−1 DB, respectively) and antihypertensive (IC50 of 28.51 µg mL−1) potential. By peptidomics and in silico analysis, 43 unique sequences of interest were found, among which LVV-Hemorphin-7 was identified. This hemorphin was demonstrated as the main responsible for the observed bioactivity. Complementary results showed a prebiotic effect mainly for the growth of Bifidobacterium animalis Bo, as well as interesting free amino acids (mainly glutamic acid, leucine, alanine, phenylalanine and aspartic acid) and mineral (e.g., Ca, Mg, P, K and Na) profiles. No antibacterial effect was verified for the seven pathogenic bacteria tested. This study allowed obtaining new ingredient of high nutritional and nutraceutical value for human consumption, with a perspective of sustainability and industrial viability. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08 2022-08-01T00:00:00Z 2023-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/38115 |
url |
http://hdl.handle.net/10400.14/38115 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0963-9969 10.1016/j.foodres.2022.111549 85132907959 35840243 000826360000001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132033360855040 |