Self mutagens affect detrimentally PCR analysis of food fungi by creating potential mutants

Detalhes bibliográficos
Autor(a) principal: Paterson, R. R. M.
Data de Publicação: 2014
Outros Autores: Lima, Nelson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/25535
Resumo: Versão aceite em 2013
id RCAP_65419281ef4c7b972d0befaedaf9971b
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/25535
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Self mutagens affect detrimentally PCR analysis of food fungi by creating potential mutantsMutagensMutationPCRFood fungiAflatoxinsMycotoxinsScience & TechnologyVersão aceite em 2013Analysing fungi from food by PCR is increasing rapidly. However, food fungi produce mutagens which may mutate the fungi in culture so that fungi which produce mycotoxins may have a negative PCR result for genes in the mycotoxin metabolic pathway and vice versa: It is impossible to state unequivocally that the current PCR results obtained are accurate. For example, food containing a mycotoxin fungus may be considered safe if the isolates from the food were mutated into being negative for the mycotoxin gene and vice versa. Growth conditions affect which mutagens are produced and the conditions used by previous authors are assessed for the first time in the current report. Previous research assumed that NA was unaffected by how fungi were grown despite no supporting evidence. Individual research groups used similar growth conditions for disparate fungi for PCR analysis which were different from methods used by alternative workers. Rationales for using particular growth methods are unexplained. The fungi will be in almost continuous contact for long periods with various biochemical mutagens at high concentrations. Only partial solutions can be provided by suggesting alternative methods. Future methods need to state why particular conditions are employed when growing these fungi and what was done to avoid mutagens.ElsevierElsevier BVUniversidade do MinhoPaterson, R. R. M.Lima, Nelson20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/25535eng0956-713510.1016/j.foodcont.2013.07.024www.elsevier.com/locate/foodcontinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:58:42Zoai:repositorium.sdum.uminho.pt:1822/25535Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:48:27.533501Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Self mutagens affect detrimentally PCR analysis of food fungi by creating potential mutants
title Self mutagens affect detrimentally PCR analysis of food fungi by creating potential mutants
spellingShingle Self mutagens affect detrimentally PCR analysis of food fungi by creating potential mutants
Paterson, R. R. M.
Mutagens
Mutation
PCR
Food fungi
Aflatoxins
Mycotoxins
Science & Technology
title_short Self mutagens affect detrimentally PCR analysis of food fungi by creating potential mutants
title_full Self mutagens affect detrimentally PCR analysis of food fungi by creating potential mutants
title_fullStr Self mutagens affect detrimentally PCR analysis of food fungi by creating potential mutants
title_full_unstemmed Self mutagens affect detrimentally PCR analysis of food fungi by creating potential mutants
title_sort Self mutagens affect detrimentally PCR analysis of food fungi by creating potential mutants
author Paterson, R. R. M.
author_facet Paterson, R. R. M.
Lima, Nelson
author_role author
author2 Lima, Nelson
author2_role author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Paterson, R. R. M.
Lima, Nelson
dc.subject.por.fl_str_mv Mutagens
Mutation
PCR
Food fungi
Aflatoxins
Mycotoxins
Science & Technology
topic Mutagens
Mutation
PCR
Food fungi
Aflatoxins
Mycotoxins
Science & Technology
description Versão aceite em 2013
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/25535
url http://hdl.handle.net/1822/25535
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0956-7135
10.1016/j.foodcont.2013.07.024
www.elsevier.com/locate/foodcont
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
Elsevier BV
publisher.none.fl_str_mv Elsevier
Elsevier BV
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132246465052672

Registros relacionados