Mutagens manufactured in fungal culture may affect DNA/RNA of producing fungi

Detalhes bibliográficos
Autor(a) principal: Paterson, R. R. M.
Data de Publicação: 2009
Outros Autores: Lima, Nelson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/9217
Resumo: Self-produced mutagens in culture by fungi may affect DNA analysis of the same fungi. This has not been considered previously. Many fungi produce numerous mutagenic secondary metabolites (SM) in culture. There is a paradox of growing fungi in media to produce representative DNA which also support mutagenic SM. This is a crucial issue in developing diagnostic and phylogenetic methods, especially for closely-related fungi. For example, idh gene analysis of the patulin metabolic pathway in fungi can be interpreted as producing some false negative and positive results in terms of possession, or nonpossession, of the gene from mutated strains. The most obvious mycotoxins and fungi to consider in this regard are aflatoxins and Aspergillus, as aflatoxins are the most mutagenic natural compounds. Many other fungi and SM are relevant. Conditions to grow fungi have not been selected to inhibit SM production although relevant data exist. In fact, fungi repair damaged nucleic acid (NA) and are capable of removing toxins by employing transporter proteins. These and NA repair mechanisms could be inhibited by secondary metabolites. Mutagenic effects may involve inhibition of DNA stabilizing enzymes. There may be an equivalent situation for bacteria. Researchers need to devise methods to reduce SM for valid protocols. More work on how mutagens affect the NA of producing fungus in vitro is required. The current review assesses the potential seriousness of the situation with selected papers.
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spelling Mutagens manufactured in fungal culture may affect DNA/RNA of producing fungiDiagnosticsDNAFungiMutagensPhylogeneticsSecondary metabolitesSelf - protectionSelf-produced mutagens in culture by fungi may affect DNA analysis of the same fungi. This has not been considered previously. Many fungi produce numerous mutagenic secondary metabolites (SM) in culture. There is a paradox of growing fungi in media to produce representative DNA which also support mutagenic SM. This is a crucial issue in developing diagnostic and phylogenetic methods, especially for closely-related fungi. For example, idh gene analysis of the patulin metabolic pathway in fungi can be interpreted as producing some false negative and positive results in terms of possession, or nonpossession, of the gene from mutated strains. The most obvious mycotoxins and fungi to consider in this regard are aflatoxins and Aspergillus, as aflatoxins are the most mutagenic natural compounds. Many other fungi and SM are relevant. Conditions to grow fungi have not been selected to inhibit SM production although relevant data exist. In fact, fungi repair damaged nucleic acid (NA) and are capable of removing toxins by employing transporter proteins. These and NA repair mechanisms could be inhibited by secondary metabolites. Mutagenic effects may involve inhibition of DNA stabilizing enzymes. There may be an equivalent situation for bacteria. Researchers need to devise methods to reduce SM for valid protocols. More work on how mutagens affect the NA of producing fungus in vitro is required. The current review assesses the potential seriousness of the situation with selected papers.IOI Professorial Chair, Department of Plant Protection, Universiti Putra Malaysia, MalaysiaFundação para a Ciência e a Tecnologia (FCT)The Society for Applied MicrobiologyUniversidade do MinhoPaterson, R. R. M.Lima, Nelson20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/9217eng"Journal of Applied Microbiology". 106:4 (2009) ISSN 1364-5072. 1070-1080.1364-507210.1111/j.1365-2672.2008.04024.x19291250info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:54Zoai:repositorium.sdum.uminho.pt:1822/9217Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:52.119541Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Mutagens manufactured in fungal culture may affect DNA/RNA of producing fungi
title Mutagens manufactured in fungal culture may affect DNA/RNA of producing fungi
spellingShingle Mutagens manufactured in fungal culture may affect DNA/RNA of producing fungi
Paterson, R. R. M.
Diagnostics
DNA
Fungi
Mutagens
Phylogenetics
Secondary metabolites
Self - protection
title_short Mutagens manufactured in fungal culture may affect DNA/RNA of producing fungi
title_full Mutagens manufactured in fungal culture may affect DNA/RNA of producing fungi
title_fullStr Mutagens manufactured in fungal culture may affect DNA/RNA of producing fungi
title_full_unstemmed Mutagens manufactured in fungal culture may affect DNA/RNA of producing fungi
title_sort Mutagens manufactured in fungal culture may affect DNA/RNA of producing fungi
author Paterson, R. R. M.
author_facet Paterson, R. R. M.
Lima, Nelson
author_role author
author2 Lima, Nelson
author2_role author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Paterson, R. R. M.
Lima, Nelson
dc.subject.por.fl_str_mv Diagnostics
DNA
Fungi
Mutagens
Phylogenetics
Secondary metabolites
Self - protection
topic Diagnostics
DNA
Fungi
Mutagens
Phylogenetics
Secondary metabolites
Self - protection
description Self-produced mutagens in culture by fungi may affect DNA analysis of the same fungi. This has not been considered previously. Many fungi produce numerous mutagenic secondary metabolites (SM) in culture. There is a paradox of growing fungi in media to produce representative DNA which also support mutagenic SM. This is a crucial issue in developing diagnostic and phylogenetic methods, especially for closely-related fungi. For example, idh gene analysis of the patulin metabolic pathway in fungi can be interpreted as producing some false negative and positive results in terms of possession, or nonpossession, of the gene from mutated strains. The most obvious mycotoxins and fungi to consider in this regard are aflatoxins and Aspergillus, as aflatoxins are the most mutagenic natural compounds. Many other fungi and SM are relevant. Conditions to grow fungi have not been selected to inhibit SM production although relevant data exist. In fact, fungi repair damaged nucleic acid (NA) and are capable of removing toxins by employing transporter proteins. These and NA repair mechanisms could be inhibited by secondary metabolites. Mutagenic effects may involve inhibition of DNA stabilizing enzymes. There may be an equivalent situation for bacteria. Researchers need to devise methods to reduce SM for valid protocols. More work on how mutagens affect the NA of producing fungus in vitro is required. The current review assesses the potential seriousness of the situation with selected papers.
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/9217
url http://hdl.handle.net/1822/9217
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of Applied Microbiology". 106:4 (2009) ISSN 1364-5072. 1070-1080.
1364-5072
10.1111/j.1365-2672.2008.04024.x
19291250
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Society for Applied Microbiology
publisher.none.fl_str_mv The Society for Applied Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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