Robustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia coli
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/36308 |
Resumo: | Portuguese Foundation for Science and Technology provided funding to Jose Fonseca under grant number PTDC/BBB-MET/1084/2012. Academy of Finland vertical bar Biotieteiden ja Ympariston Tutkimuksen Toimikunta (Forskningsradet for Biovetenskap och Miljo) provided funding to Andre S. Ribeiro under grant number 257603. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Robustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia coliCell DivisionEscherichia coliEscherichia coli ProteinsGenes, ReporterLuminescent ProteinsMicroscopy, FluorescenceProtein AggregatesStaining and LabelingTime-Lapse ImagingJournal ArticleResearch Support, Non-U.S. Gov'tPortuguese Foundation for Science and Technology provided funding to Jose Fonseca under grant number PTDC/BBB-MET/1084/2012. Academy of Finland vertical bar Biotieteiden ja Ympariston Tutkimuksen Toimikunta (Forskningsradet for Biovetenskap och Miljo) provided funding to Andre S. Ribeiro under grant number 257603.UNLABELLED: Escherichia coli segregates protein aggregates to the poles by nucleoid exclusion. Combined with cell divisions, this generates heterogeneous aggregate distributions in subsequent cell generations. We studied the robustness of this process with differing medium richness and antibiotics stress, which affect nucleoid size, using multimodal, time-lapse microscopy of live cells expressing both a fluorescently tagged chaperone (IbpA), which identifies in vivo the location of aggregates, and HupA-mCherry, a fluorescent variant of a nucleoid-associated protein. We find that the relative sizes of the nucleoid's major and minor axes change widely, in a positively correlated fashion, with medium richness and antibiotic stress. The aggregate's distribution along the major cell axis also changes between conditions and in agreement with the nucleoid exclusion phenomenon. Consequently, the fraction of aggregates at the midcell region prior to cell division differs between conditions, which will affect the degree of asymmetries in the partitioning of aggregates between cells of future generations. Finally, from the location of the peak of anisotropy in the aggregate displacement distribution, the nucleoid relative size, and the spatiotemporal aggregate distribution, we find that the exclusion of detectable aggregates from midcell is most pronounced in cells with mid-sized nucleoids, which are most common under optimal conditions. We conclude that the aggregate management mechanisms of E. coli are significantly robust but are not immune to stresses due to the tangible effect that these have on nucleoid size. IMPORTANCE: Escherichia coli segregates protein aggregates to the poles by nucleoid exclusion. From live single-cell microscopy studies of the robustness of this process to various stresses known to affect nucleoid size, we find that nucleoid size and aggregate preferential locations change concordantly between conditions. Also, the degree of influence of the nucleoid on aggregate positioning differs between conditions, causing aggregate numbers at midcell to differ in cell division events, which will affect the degree of asymmetries in the partitioning of aggregates between cells of future generations. Finally, we find that aggregate segregation to the cell poles is most pronounced in cells with mid-sized nucleoids. We conclude that the energy-free process of the midcell exclusion of aggregates partially loses effectiveness under stressful conditions.UNINOVA-Instituto de Desenvolvimento de Novas TecnologiasRUNNeeli-Venkata, RamakanthMartikainen, AnttiGupta, AbhishekhGonçalves, NadiaFonseca, JoseRibeiro, André S.2018-05-09T22:03:53Z2016-032016-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://hdl.handle.net/10362/36308eng0021-9193PURE: 1264601https://doi.org/10.1128/JB.00848-15info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:19:41Zoai:run.unl.pt:10362/36308Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:26.237030Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Robustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia coli |
| title |
Robustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia coli |
| spellingShingle |
Robustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia coli Neeli-Venkata, Ramakanth Cell Division Escherichia coli Escherichia coli Proteins Genes, Reporter Luminescent Proteins Microscopy, Fluorescence Protein Aggregates Staining and Labeling Time-Lapse Imaging Journal Article Research Support, Non-U.S. Gov't |
| title_short |
Robustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia coli |
| title_full |
Robustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia coli |
| title_fullStr |
Robustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia coli |
| title_full_unstemmed |
Robustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia coli |
| title_sort |
Robustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia coli |
| author |
Neeli-Venkata, Ramakanth |
| author_facet |
Neeli-Venkata, Ramakanth Martikainen, Antti Gupta, Abhishekh Gonçalves, Nadia Fonseca, Jose Ribeiro, André S. |
| author_role |
author |
| author2 |
Martikainen, Antti Gupta, Abhishekh Gonçalves, Nadia Fonseca, Jose Ribeiro, André S. |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias RUN |
| dc.contributor.author.fl_str_mv |
Neeli-Venkata, Ramakanth Martikainen, Antti Gupta, Abhishekh Gonçalves, Nadia Fonseca, Jose Ribeiro, André S. |
| dc.subject.por.fl_str_mv |
Cell Division Escherichia coli Escherichia coli Proteins Genes, Reporter Luminescent Proteins Microscopy, Fluorescence Protein Aggregates Staining and Labeling Time-Lapse Imaging Journal Article Research Support, Non-U.S. Gov't |
| topic |
Cell Division Escherichia coli Escherichia coli Proteins Genes, Reporter Luminescent Proteins Microscopy, Fluorescence Protein Aggregates Staining and Labeling Time-Lapse Imaging Journal Article Research Support, Non-U.S. Gov't |
| description |
Portuguese Foundation for Science and Technology provided funding to Jose Fonseca under grant number PTDC/BBB-MET/1084/2012. Academy of Finland vertical bar Biotieteiden ja Ympariston Tutkimuksen Toimikunta (Forskningsradet for Biovetenskap och Miljo) provided funding to Andre S. Ribeiro under grant number 257603. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016-03 2016-03-01T00:00:00Z 2018-05-09T22:03:53Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/36308 |
| url |
http://hdl.handle.net/10362/36308 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
0021-9193 PURE: 1264601 https://doi.org/10.1128/JB.00848-15 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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9 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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