Investigation of cerebellar outputs for adapting locomotion
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/162064 |
Resumo: | Every time we are exposed to new perturbations that affect our walking, we have to adjust in time and space to ensure a coordinated gait. This requires locomotor learning which can be studied using a split-belt treadmill, where each side of the body can move at different speeds. Within minutes, humans learn to regain a more symmetric gait in response to the split-belt perturbation. Previous findings obtained in the Carey lab, where the present project was carried out, demonstrated that this behavior is conserved in mice and requires the intermediate cerebellum. The goal of this study is to unravel the circuits, downstream of the interposed, that carry spatial and/ or temporal calibration signals to the limbs so as to correct movement. We employ the split-belt treadmill paradigm to test if spatial and temporal adaptation relies on distinct cerebellar output circuits. This was done using mice lacking specific subpopulations of interposed neurons projecting to downstream regions. We analyzed the functional impact of different interposed circuits in both spatial and temporal locomotor adaptation. Since the behavior is conserved across vertebrates and is currently used in rehabilitation therapy, these results may help refine or create new strategies to treat certain gait asymmetries. |
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Investigation of cerebellar outputs for adapting locomotionAdaptationLocomotionCerebellumNeural circuitsMouseDomínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e TecnologiasEvery time we are exposed to new perturbations that affect our walking, we have to adjust in time and space to ensure a coordinated gait. This requires locomotor learning which can be studied using a split-belt treadmill, where each side of the body can move at different speeds. Within minutes, humans learn to regain a more symmetric gait in response to the split-belt perturbation. Previous findings obtained in the Carey lab, where the present project was carried out, demonstrated that this behavior is conserved in mice and requires the intermediate cerebellum. The goal of this study is to unravel the circuits, downstream of the interposed, that carry spatial and/ or temporal calibration signals to the limbs so as to correct movement. We employ the split-belt treadmill paradigm to test if spatial and temporal adaptation relies on distinct cerebellar output circuits. This was done using mice lacking specific subpopulations of interposed neurons projecting to downstream regions. We analyzed the functional impact of different interposed circuits in both spatial and temporal locomotor adaptation. Since the behavior is conserved across vertebrates and is currently used in rehabilitation therapy, these results may help refine or create new strategies to treat certain gait asymmetries.Sempre que somos expostos a novas perturbações que afetam a marcha, temos de nos ajustar no tempo e no espaço para garantir uma marcha mais coordenada. Isto requer uma aprendizagem locomotora que pode ser estudada utilizando uma passadeira com tapete rolante dividido, de modo a que cada lado do corpo se possa mover a velocidades diferentes. Em poucos minutos, os seres humanos aprendem a recuperar uma marcha mais simétrica em resposta à perturbação da cinta dividida. Resultados anteriormente obtidos no Carey Lab, onde o presente projeto foi realizado, demonstram que este comportamento é conservado em ratinhos e requer o cerebelo. O objetivo deste estudo é revelar os circuitos, a jusante do interposed nucleus, que transportam os sinais de calibração espacial e/ ou temporal para os membros, de modo a corrigir o movimento. Foi aplicado este paradigma que recorre a uma passadeira com tapete rolante dividido para testar se a adaptação espacial e temporal depende de diferentes cerebellar output circuits. Para tal, utilizámos ratinhos que apresentam défice de certas subpopulações específicas de neurónios do interposed nucleus que projetam para outras regiões no cérebro. Analisáamos o impacto funcional de diferentes circuitos neuronais com origem no interposed na adaptação locomotora espacial e temporal. Uma vez que o comportamento é conservado entre vertebrados e atualmente utilizado em terapia de reabilitação, os resultados adquiridos neste projeto poderão, em última análise, ajudar a melhorar ou criar novas estratégias destinadas a tratar certas assimetrias da marcha.Carey, MeganVigário, RicardoRUNMatos, Matilde Costa Coelho De Saldanha2023-12-112026-11-23T00:00:00Z2023-12-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/162064enginfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:44:52Zoai:run.unl.pt:10362/162064Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:58:44.598767Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Investigation of cerebellar outputs for adapting locomotion |
title |
Investigation of cerebellar outputs for adapting locomotion |
spellingShingle |
Investigation of cerebellar outputs for adapting locomotion Matos, Matilde Costa Coelho De Saldanha Adaptation Locomotion Cerebellum Neural circuits Mouse Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
title_short |
Investigation of cerebellar outputs for adapting locomotion |
title_full |
Investigation of cerebellar outputs for adapting locomotion |
title_fullStr |
Investigation of cerebellar outputs for adapting locomotion |
title_full_unstemmed |
Investigation of cerebellar outputs for adapting locomotion |
title_sort |
Investigation of cerebellar outputs for adapting locomotion |
author |
Matos, Matilde Costa Coelho De Saldanha |
author_facet |
Matos, Matilde Costa Coelho De Saldanha |
author_role |
author |
dc.contributor.none.fl_str_mv |
Carey, Megan Vigário, Ricardo RUN |
dc.contributor.author.fl_str_mv |
Matos, Matilde Costa Coelho De Saldanha |
dc.subject.por.fl_str_mv |
Adaptation Locomotion Cerebellum Neural circuits Mouse Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
topic |
Adaptation Locomotion Cerebellum Neural circuits Mouse Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
description |
Every time we are exposed to new perturbations that affect our walking, we have to adjust in time and space to ensure a coordinated gait. This requires locomotor learning which can be studied using a split-belt treadmill, where each side of the body can move at different speeds. Within minutes, humans learn to regain a more symmetric gait in response to the split-belt perturbation. Previous findings obtained in the Carey lab, where the present project was carried out, demonstrated that this behavior is conserved in mice and requires the intermediate cerebellum. The goal of this study is to unravel the circuits, downstream of the interposed, that carry spatial and/ or temporal calibration signals to the limbs so as to correct movement. We employ the split-belt treadmill paradigm to test if spatial and temporal adaptation relies on distinct cerebellar output circuits. This was done using mice lacking specific subpopulations of interposed neurons projecting to downstream regions. We analyzed the functional impact of different interposed circuits in both spatial and temporal locomotor adaptation. Since the behavior is conserved across vertebrates and is currently used in rehabilitation therapy, these results may help refine or create new strategies to treat certain gait asymmetries. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-11 2023-12-11T00:00:00Z 2026-11-23T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/162064 |
url |
http://hdl.handle.net/10362/162064 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799138168027480064 |