Proteomic analysis of the hydrogen and carbon monoxide metabolism of Methanothermobacter marburgensis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/43295 |
Resumo: | Hydrogenotrophic methanogenic archaea are efficient H2 utilizers, but only a few are known to be able to utilize CO. Methanothermobacter thermoautotrophicus is one of the hydrogenotrophic methanogens able to grow on CO, albeit about 100 times slower than on H2 + CO2. In this study we show that the hydrogenotrophic methanogen Methanothermobacter marburgensis, is able to perform methanogenic growth on H2/CO2/CO and on CO as a sole substrate. To gain further insight in its carboxydotrophic metabolism, the proteome of M. marburgensis, grown on H2/CO2 and H2/CO2/CO, was analysed. Cultures grown with H2/CO2/CO showed relative higher abundance of enzymes involved in the reductive acetyl-CoA pathway and proteins involved in redox metabolism. The data suggest that the strong reducing capacity of CO negatively affects hydrogenotrophic methanogenesis, making growth on CO as a sole substrate difficult for this type of methanogens. M. marburgensis appears to partly deal with this by upregulating co-factor regenerating reactions and activating additional pathways allowing for formation of other products, like acetate. |
id |
RCAP_66856a851c22bb16eb72dd491881b444 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/43295 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Proteomic analysis of the hydrogen and carbon monoxide metabolism of Methanothermobacter marburgensissyngasmethanogenesisCODHReductive acetyl-CoA pathwayMethanothermobacter thermoautotrophicusScience & TechnologyHydrogenotrophic methanogenic archaea are efficient H2 utilizers, but only a few are known to be able to utilize CO. Methanothermobacter thermoautotrophicus is one of the hydrogenotrophic methanogens able to grow on CO, albeit about 100 times slower than on H2 + CO2. In this study we show that the hydrogenotrophic methanogen Methanothermobacter marburgensis, is able to perform methanogenic growth on H2/CO2/CO and on CO as a sole substrate. To gain further insight in its carboxydotrophic metabolism, the proteome of M. marburgensis, grown on H2/CO2 and H2/CO2/CO, was analysed. Cultures grown with H2/CO2/CO showed relative higher abundance of enzymes involved in the reductive acetyl-CoA pathway and proteins involved in redox metabolism. The data suggest that the strong reducing capacity of CO negatively affects hydrogenotrophic methanogenesis, making growth on CO as a sole substrate difficult for this type of methanogens. M. marburgensis appears to partly deal with this by upregulating co-factor regenerating reactions and activating additional pathways allowing for formation of other products, like acetate.Research of AS is supported by an ERC grant (project 323009) and a Gravitation grant (project024.002.002)of the Netherlands Ministry of Education, Culture and Science and the Netherlands Science Foundation (NWO).Frontiers MediaUniversidade do MinhoDiender, MartijnPereira, Ricardo A. G.Wessels, Hans J. C. T.Stams, Alfons Johannes MariaSousa, Diana Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/43295engDiender, Martijn; Pereira, Ricardo A. G.; Wessels, Hans J. C. T.; Stams, A. J. M.; Sousa, Diana Z, Proteomic analysis of the hydrogen and carbon monoxide metabolism of Methanothermobacter marburgensis. Frontiers in Microbiology, 7(1049), 1-10, 20161664-302X1664-302X10.3389/fmicb.2016.01049http://journal.frontiersin.org/journal/microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:53:59Zoai:repositorium.sdum.uminho.pt:1822/43295Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:53:30.075922Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Proteomic analysis of the hydrogen and carbon monoxide metabolism of Methanothermobacter marburgensis |
title |
Proteomic analysis of the hydrogen and carbon monoxide metabolism of Methanothermobacter marburgensis |
spellingShingle |
Proteomic analysis of the hydrogen and carbon monoxide metabolism of Methanothermobacter marburgensis Diender, Martijn syngas methanogenesis CODH Reductive acetyl-CoA pathway Methanothermobacter thermoautotrophicus Science & Technology |
title_short |
Proteomic analysis of the hydrogen and carbon monoxide metabolism of Methanothermobacter marburgensis |
title_full |
Proteomic analysis of the hydrogen and carbon monoxide metabolism of Methanothermobacter marburgensis |
title_fullStr |
Proteomic analysis of the hydrogen and carbon monoxide metabolism of Methanothermobacter marburgensis |
title_full_unstemmed |
Proteomic analysis of the hydrogen and carbon monoxide metabolism of Methanothermobacter marburgensis |
title_sort |
Proteomic analysis of the hydrogen and carbon monoxide metabolism of Methanothermobacter marburgensis |
author |
Diender, Martijn |
author_facet |
Diender, Martijn Pereira, Ricardo A. G. Wessels, Hans J. C. T. Stams, Alfons Johannes Maria Sousa, Diana Z |
author_role |
author |
author2 |
Pereira, Ricardo A. G. Wessels, Hans J. C. T. Stams, Alfons Johannes Maria Sousa, Diana Z |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Diender, Martijn Pereira, Ricardo A. G. Wessels, Hans J. C. T. Stams, Alfons Johannes Maria Sousa, Diana Z |
dc.subject.por.fl_str_mv |
syngas methanogenesis CODH Reductive acetyl-CoA pathway Methanothermobacter thermoautotrophicus Science & Technology |
topic |
syngas methanogenesis CODH Reductive acetyl-CoA pathway Methanothermobacter thermoautotrophicus Science & Technology |
description |
Hydrogenotrophic methanogenic archaea are efficient H2 utilizers, but only a few are known to be able to utilize CO. Methanothermobacter thermoautotrophicus is one of the hydrogenotrophic methanogens able to grow on CO, albeit about 100 times slower than on H2 + CO2. In this study we show that the hydrogenotrophic methanogen Methanothermobacter marburgensis, is able to perform methanogenic growth on H2/CO2/CO and on CO as a sole substrate. To gain further insight in its carboxydotrophic metabolism, the proteome of M. marburgensis, grown on H2/CO2 and H2/CO2/CO, was analysed. Cultures grown with H2/CO2/CO showed relative higher abundance of enzymes involved in the reductive acetyl-CoA pathway and proteins involved in redox metabolism. The data suggest that the strong reducing capacity of CO negatively affects hydrogenotrophic methanogenesis, making growth on CO as a sole substrate difficult for this type of methanogens. M. marburgensis appears to partly deal with this by upregulating co-factor regenerating reactions and activating additional pathways allowing for formation of other products, like acetate. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/43295 |
url |
http://hdl.handle.net/1822/43295 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Diender, Martijn; Pereira, Ricardo A. G.; Wessels, Hans J. C. T.; Stams, A. J. M.; Sousa, Diana Z, Proteomic analysis of the hydrogen and carbon monoxide metabolism of Methanothermobacter marburgensis. Frontiers in Microbiology, 7(1049), 1-10, 2016 1664-302X 1664-302X 10.3389/fmicb.2016.01049 http://journal.frontiersin.org/journal/microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133131685494784 |