Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies

Detalhes bibliográficos
Autor(a) principal: Melo, Luís D. R.
Data de Publicação: 2018
Outros Autores: França, Angela, Brandão, Ana Catarina, Sillankorva, Sanna, Cerca, Nuno, Azeredo, Joana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/59535
Resumo: The majority of phage infection studies are performed in bacteria that are growing exponentially, although in nature, phages usually interact also with non-replicating cells. These stationary-phase cells differ from exponential cells morphologically, physiologically and metabolically. The interaction of a Sep1virus with Staphylococcus epidermidis stationary and exponential phase cells was explored. Phage SEP1 efficiently infected both cell culture states, without the addition of any fresh nutrients to stationary cultures. Phage-host interactions, analysed by flow cytometry, showed stationary-phase cells response to phage immediately after SEP1 addition. Quantitative PCR experiments corroborate that phage genes are expressed within 5 min of contact with stationary phase cells. The increase of host RNA polymerase transcripts in stationary cells, suggests that SEP1 infection leads to upregulation of host machinery fundamental for completion of its lytic life cycle. SEP1 infection and replication process highlights its potential clinical interest targeting stationary phase cells.
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spelling Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studiesStaphylococcus epidermidisbacteriophageflow cytometrystationary cellsgene expressionphage-host interactionsScience & TechnologyThe majority of phage infection studies are performed in bacteria that are growing exponentially, although in nature, phages usually interact also with non-replicating cells. These stationary-phase cells differ from exponential cells morphologically, physiologically and metabolically. The interaction of a Sep1virus with Staphylococcus epidermidis stationary and exponential phase cells was explored. Phage SEP1 efficiently infected both cell culture states, without the addition of any fresh nutrients to stationary cultures. Phage-host interactions, analysed by flow cytometry, showed stationary-phase cells response to phage immediately after SEP1 addition. Quantitative PCR experiments corroborate that phage genes are expressed within 5 min of contact with stationary phase cells. The increase of host RNA polymerase transcripts in stationary cells, suggests that SEP1 infection leads to upregulation of host machinery fundamental for completion of its lytic life cycle. SEP1 infection and replication process highlights its potential clinical interest targeting stationary phase cells.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding [UID/BIO/04469] unit and COMPETE 2020 [POCI01-0145-FEDER-006684] and BioTecNorte operation [NORTE-010145-FEDER-000004] funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. SS and NC are Investigator FCT. AF is supported by an individual FCT fellowship [SFRH/BPD/99961/2014].info:eu-repo/semantics/publishedVersionOxford University PressUniversidade do MinhoMelo, Luís D. R.França, AngelaBrandão, Ana CatarinaSillankorva, SannaCerca, NunoAzeredo, Joana2018-102018-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/59535engMelo, Luís D. R.; França, Angela; Brandão, Ana Catarina; Sillankorva, Sanna; Cerca, Nuno; Azeredo, Joana, Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies. FEMS Microbiology Ecology, 94(10), fiy143, 20180168-649610.1093/femsec/fiy14330060135https://academic.oup.com/femsecinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:42:14Zoai:repositorium.sdum.uminho.pt:1822/59535Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:39:26.503528Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies
title Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies
spellingShingle Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies
Melo, Luís D. R.
Staphylococcus epidermidis
bacteriophage
flow cytometry
stationary cells
gene expression
phage-host interactions
Science & Technology
title_short Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies
title_full Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies
title_fullStr Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies
title_full_unstemmed Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies
title_sort Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies
author Melo, Luís D. R.
author_facet Melo, Luís D. R.
França, Angela
Brandão, Ana Catarina
Sillankorva, Sanna
Cerca, Nuno
Azeredo, Joana
author_role author
author2 França, Angela
Brandão, Ana Catarina
Sillankorva, Sanna
Cerca, Nuno
Azeredo, Joana
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Melo, Luís D. R.
França, Angela
Brandão, Ana Catarina
Sillankorva, Sanna
Cerca, Nuno
Azeredo, Joana
dc.subject.por.fl_str_mv Staphylococcus epidermidis
bacteriophage
flow cytometry
stationary cells
gene expression
phage-host interactions
Science & Technology
topic Staphylococcus epidermidis
bacteriophage
flow cytometry
stationary cells
gene expression
phage-host interactions
Science & Technology
description The majority of phage infection studies are performed in bacteria that are growing exponentially, although in nature, phages usually interact also with non-replicating cells. These stationary-phase cells differ from exponential cells morphologically, physiologically and metabolically. The interaction of a Sep1virus with Staphylococcus epidermidis stationary and exponential phase cells was explored. Phage SEP1 efficiently infected both cell culture states, without the addition of any fresh nutrients to stationary cultures. Phage-host interactions, analysed by flow cytometry, showed stationary-phase cells response to phage immediately after SEP1 addition. Quantitative PCR experiments corroborate that phage genes are expressed within 5 min of contact with stationary phase cells. The increase of host RNA polymerase transcripts in stationary cells, suggests that SEP1 infection leads to upregulation of host machinery fundamental for completion of its lytic life cycle. SEP1 infection and replication process highlights its potential clinical interest targeting stationary phase cells.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
2018-10-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/59535
url http://hdl.handle.net/1822/59535
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Melo, Luís D. R.; França, Angela; Brandão, Ana Catarina; Sillankorva, Sanna; Cerca, Nuno; Azeredo, Joana, Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies. FEMS Microbiology Ecology, 94(10), fiy143, 2018
0168-6496
10.1093/femsec/fiy143
30060135
https://academic.oup.com/femsec
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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