Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/59535 |
Resumo: | The majority of phage infection studies are performed in bacteria that are growing exponentially, although in nature, phages usually interact also with non-replicating cells. These stationary-phase cells differ from exponential cells morphologically, physiologically and metabolically. The interaction of a Sep1virus with Staphylococcus epidermidis stationary and exponential phase cells was explored. Phage SEP1 efficiently infected both cell culture states, without the addition of any fresh nutrients to stationary cultures. Phage-host interactions, analysed by flow cytometry, showed stationary-phase cells response to phage immediately after SEP1 addition. Quantitative PCR experiments corroborate that phage genes are expressed within 5 min of contact with stationary phase cells. The increase of host RNA polymerase transcripts in stationary cells, suggests that SEP1 infection leads to upregulation of host machinery fundamental for completion of its lytic life cycle. SEP1 infection and replication process highlights its potential clinical interest targeting stationary phase cells. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studiesStaphylococcus epidermidisbacteriophageflow cytometrystationary cellsgene expressionphage-host interactionsScience & TechnologyThe majority of phage infection studies are performed in bacteria that are growing exponentially, although in nature, phages usually interact also with non-replicating cells. These stationary-phase cells differ from exponential cells morphologically, physiologically and metabolically. The interaction of a Sep1virus with Staphylococcus epidermidis stationary and exponential phase cells was explored. Phage SEP1 efficiently infected both cell culture states, without the addition of any fresh nutrients to stationary cultures. Phage-host interactions, analysed by flow cytometry, showed stationary-phase cells response to phage immediately after SEP1 addition. Quantitative PCR experiments corroborate that phage genes are expressed within 5 min of contact with stationary phase cells. The increase of host RNA polymerase transcripts in stationary cells, suggests that SEP1 infection leads to upregulation of host machinery fundamental for completion of its lytic life cycle. SEP1 infection and replication process highlights its potential clinical interest targeting stationary phase cells.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding [UID/BIO/04469] unit and COMPETE 2020 [POCI01-0145-FEDER-006684] and BioTecNorte operation [NORTE-010145-FEDER-000004] funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. SS and NC are Investigator FCT. AF is supported by an individual FCT fellowship [SFRH/BPD/99961/2014].info:eu-repo/semantics/publishedVersionOxford University PressUniversidade do MinhoMelo, Luís D. R.França, AngelaBrandão, Ana CatarinaSillankorva, SannaCerca, NunoAzeredo, Joana2018-102018-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/59535engMelo, Luís D. R.; França, Angela; Brandão, Ana Catarina; Sillankorva, Sanna; Cerca, Nuno; Azeredo, Joana, Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies. FEMS Microbiology Ecology, 94(10), fiy143, 20180168-649610.1093/femsec/fiy14330060135https://academic.oup.com/femsecinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:42:14Zoai:repositorium.sdum.uminho.pt:1822/59535Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:39:26.503528Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies |
title |
Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies |
spellingShingle |
Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies Melo, Luís D. R. Staphylococcus epidermidis bacteriophage flow cytometry stationary cells gene expression phage-host interactions Science & Technology |
title_short |
Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies |
title_full |
Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies |
title_fullStr |
Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies |
title_full_unstemmed |
Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies |
title_sort |
Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies |
author |
Melo, Luís D. R. |
author_facet |
Melo, Luís D. R. França, Angela Brandão, Ana Catarina Sillankorva, Sanna Cerca, Nuno Azeredo, Joana |
author_role |
author |
author2 |
França, Angela Brandão, Ana Catarina Sillankorva, Sanna Cerca, Nuno Azeredo, Joana |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Melo, Luís D. R. França, Angela Brandão, Ana Catarina Sillankorva, Sanna Cerca, Nuno Azeredo, Joana |
dc.subject.por.fl_str_mv |
Staphylococcus epidermidis bacteriophage flow cytometry stationary cells gene expression phage-host interactions Science & Technology |
topic |
Staphylococcus epidermidis bacteriophage flow cytometry stationary cells gene expression phage-host interactions Science & Technology |
description |
The majority of phage infection studies are performed in bacteria that are growing exponentially, although in nature, phages usually interact also with non-replicating cells. These stationary-phase cells differ from exponential cells morphologically, physiologically and metabolically. The interaction of a Sep1virus with Staphylococcus epidermidis stationary and exponential phase cells was explored. Phage SEP1 efficiently infected both cell culture states, without the addition of any fresh nutrients to stationary cultures. Phage-host interactions, analysed by flow cytometry, showed stationary-phase cells response to phage immediately after SEP1 addition. Quantitative PCR experiments corroborate that phage genes are expressed within 5 min of contact with stationary phase cells. The increase of host RNA polymerase transcripts in stationary cells, suggests that SEP1 infection leads to upregulation of host machinery fundamental for completion of its lytic life cycle. SEP1 infection and replication process highlights its potential clinical interest targeting stationary phase cells. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10 2018-10-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/59535 |
url |
http://hdl.handle.net/1822/59535 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Melo, Luís D. R.; França, Angela; Brandão, Ana Catarina; Sillankorva, Sanna; Cerca, Nuno; Azeredo, Joana, Assessment of Sep1virus interaction with stationary cultures by transcriptional and flow cytometry studies. FEMS Microbiology Ecology, 94(10), fiy143, 2018 0168-6496 10.1093/femsec/fiy143 30060135 https://academic.oup.com/femsec |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Oxford University Press |
publisher.none.fl_str_mv |
Oxford University Press |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132935736000512 |