Ascorbate prevents prooxidant effects of vanadium pentoxide on wildtype Saccharomyces cerevisiae UEME3

Detalhes bibliográficos
Autor(a) principal: Gonçalves, S
Data de Publicação: 2010
Outros Autores: Ferreira, R, Alves-Pereira, I
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/5955
https://doi.org/10.1016/j.toxlet.2010.03.962
Resumo: Vanadiumpentoxide,V2O5, (V5+) themosttoxiccompoundof vanadium behaves as an amphoteric oxide and a powerful oxidizing agent which may be an oxidative stress inducer. The vanadium (V5+) is generally reduced by living cells to vanadium (V4+), less toxic, using enzymes which mobilize the reducing equivalents of NADPH, or nonenzymatically using ascorbate. Nevertheless, species generated by vanadium (V4+) from H2O2 and lipid peroxidation, via Fenton reaction can have a significant role in the metabolism of vanadium and induce cell damage in physiological conditions. Although vanadium is an element with ubiquitous environmental distribution, combustion of fossil fuels represents an important source of vanadium in the environment. Biological studies to evaluate the influence of vanadium on living organisms has shown that is mutagenic and genotoxic. Having in account that toxicity mechanisms of vanadium on eukaryotic cells are not entirely clear, the main objectives of this work was to evaluate the synergistic effects of 0.025mMascorbate vs 2mMV2O5 on cell survival, alkaline phosphatase (ALP), catalases A and T (CAT A, T) and glutathione reductase (GR) activities of Saccharomyces cerevisiae UEME3. Cells at midexponential phase were inoculated in YEPD medium with 2% (w/v) glucose and incubated during 72 h in a water bath with orbital stirring, at 28 ◦C, in the absence or in presence of 2mM V2O5, or 0.025mM ascorbate plus 2mM V2O5. Samples from each treatment were used to obtain growth curves and to prepare post12,000 g supernatant, used for enzymatic activities determination. The results shown that ascorbate counteracted growth inhibition, the decrease of ALP and CAT activities, as well as the increase of GR antioxidants activities, caused by 2mMV2O5, to values similar of control cells.
id RCAP_67dd4e797ec4e86470aeb7c74c74218d
oai_identifier_str oai:dspace.uevora.pt:10174/5955
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Ascorbate prevents prooxidant effects of vanadium pentoxide on wildtype Saccharomyces cerevisiae UEME3alkaline phosphatasecatalasesglutathione reductaseVanadiumpentoxide,V2O5, (V5+) themosttoxiccompoundof vanadium behaves as an amphoteric oxide and a powerful oxidizing agent which may be an oxidative stress inducer. The vanadium (V5+) is generally reduced by living cells to vanadium (V4+), less toxic, using enzymes which mobilize the reducing equivalents of NADPH, or nonenzymatically using ascorbate. Nevertheless, species generated by vanadium (V4+) from H2O2 and lipid peroxidation, via Fenton reaction can have a significant role in the metabolism of vanadium and induce cell damage in physiological conditions. Although vanadium is an element with ubiquitous environmental distribution, combustion of fossil fuels represents an important source of vanadium in the environment. Biological studies to evaluate the influence of vanadium on living organisms has shown that is mutagenic and genotoxic. Having in account that toxicity mechanisms of vanadium on eukaryotic cells are not entirely clear, the main objectives of this work was to evaluate the synergistic effects of 0.025mMascorbate vs 2mMV2O5 on cell survival, alkaline phosphatase (ALP), catalases A and T (CAT A, T) and glutathione reductase (GR) activities of Saccharomyces cerevisiae UEME3. Cells at midexponential phase were inoculated in YEPD medium with 2% (w/v) glucose and incubated during 72 h in a water bath with orbital stirring, at 28 ◦C, in the absence or in presence of 2mM V2O5, or 0.025mM ascorbate plus 2mM V2O5. Samples from each treatment were used to obtain growth curves and to prepare post12,000 g supernatant, used for enzymatic activities determination. The results shown that ascorbate counteracted growth inhibition, the decrease of ALP and CAT activities, as well as the increase of GR antioxidants activities, caused by 2mMV2O5, to values similar of control cells.Elsevier2012-11-23T14:29:02Z2012-11-232010-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/5955http://hdl.handle.net/10174/5955https://doi.org/10.1016/j.toxlet.2010.03.962porGonçalves S, Ferreira R, Alves-Pereira I (2010) – Ascorbate prevents prooxidant effects of vanadium pentoxide on wild-type Saccharomyces cerevisiae UE-ME3. Toxicology Letters, 196 Sup: S304-S305. (ISSN: 0378-4274, doi:10.1016/j.toxlet.2010.03.962).ICAAMndraf@uevora.ptiap@uevora.pt365Gonçalves, SFerreira, RAlves-Pereira, Iinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:45:08Zoai:dspace.uevora.pt:10174/5955Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:00:52.822691Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Ascorbate prevents prooxidant effects of vanadium pentoxide on wildtype Saccharomyces cerevisiae UEME3
title Ascorbate prevents prooxidant effects of vanadium pentoxide on wildtype Saccharomyces cerevisiae UEME3
spellingShingle Ascorbate prevents prooxidant effects of vanadium pentoxide on wildtype Saccharomyces cerevisiae UEME3
Gonçalves, S
alkaline phosphatase
catalases
glutathione reductase
title_short Ascorbate prevents prooxidant effects of vanadium pentoxide on wildtype Saccharomyces cerevisiae UEME3
title_full Ascorbate prevents prooxidant effects of vanadium pentoxide on wildtype Saccharomyces cerevisiae UEME3
title_fullStr Ascorbate prevents prooxidant effects of vanadium pentoxide on wildtype Saccharomyces cerevisiae UEME3
title_full_unstemmed Ascorbate prevents prooxidant effects of vanadium pentoxide on wildtype Saccharomyces cerevisiae UEME3
title_sort Ascorbate prevents prooxidant effects of vanadium pentoxide on wildtype Saccharomyces cerevisiae UEME3
author Gonçalves, S
author_facet Gonçalves, S
Ferreira, R
Alves-Pereira, I
author_role author
author2 Ferreira, R
Alves-Pereira, I
author2_role author
author
dc.contributor.author.fl_str_mv Gonçalves, S
Ferreira, R
Alves-Pereira, I
dc.subject.por.fl_str_mv alkaline phosphatase
catalases
glutathione reductase
topic alkaline phosphatase
catalases
glutathione reductase
description Vanadiumpentoxide,V2O5, (V5+) themosttoxiccompoundof vanadium behaves as an amphoteric oxide and a powerful oxidizing agent which may be an oxidative stress inducer. The vanadium (V5+) is generally reduced by living cells to vanadium (V4+), less toxic, using enzymes which mobilize the reducing equivalents of NADPH, or nonenzymatically using ascorbate. Nevertheless, species generated by vanadium (V4+) from H2O2 and lipid peroxidation, via Fenton reaction can have a significant role in the metabolism of vanadium and induce cell damage in physiological conditions. Although vanadium is an element with ubiquitous environmental distribution, combustion of fossil fuels represents an important source of vanadium in the environment. Biological studies to evaluate the influence of vanadium on living organisms has shown that is mutagenic and genotoxic. Having in account that toxicity mechanisms of vanadium on eukaryotic cells are not entirely clear, the main objectives of this work was to evaluate the synergistic effects of 0.025mMascorbate vs 2mMV2O5 on cell survival, alkaline phosphatase (ALP), catalases A and T (CAT A, T) and glutathione reductase (GR) activities of Saccharomyces cerevisiae UEME3. Cells at midexponential phase were inoculated in YEPD medium with 2% (w/v) glucose and incubated during 72 h in a water bath with orbital stirring, at 28 ◦C, in the absence or in presence of 2mM V2O5, or 0.025mM ascorbate plus 2mM V2O5. Samples from each treatment were used to obtain growth curves and to prepare post12,000 g supernatant, used for enzymatic activities determination. The results shown that ascorbate counteracted growth inhibition, the decrease of ALP and CAT activities, as well as the increase of GR antioxidants activities, caused by 2mMV2O5, to values similar of control cells.
publishDate 2010
dc.date.none.fl_str_mv 2010-07-01T00:00:00Z
2012-11-23T14:29:02Z
2012-11-23
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/5955
http://hdl.handle.net/10174/5955
https://doi.org/10.1016/j.toxlet.2010.03.962
url http://hdl.handle.net/10174/5955
https://doi.org/10.1016/j.toxlet.2010.03.962
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Gonçalves S, Ferreira R, Alves-Pereira I (2010) – Ascorbate prevents prooxidant effects of vanadium pentoxide on wild-type Saccharomyces cerevisiae UE-ME3. Toxicology Letters, 196 Sup: S304-S305. (ISSN: 0378-4274, doi:10.1016/j.toxlet.2010.03.962).
ICAAM
nd
raf@uevora.pt
iap@uevora.pt
365
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799136491766546432