Effect of the morphogene bolA on the permeability of the Escherichia coli outer membrane

Detalhes bibliográficos
Autor(a) principal: Freire, Patrick
Data de Publicação: 2006
Outros Autores: Vieira, Helena L.A., Furtado, Ana R., De Pedro, Miguel A., Arraiano, Cecília M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/147414
Resumo: Escherichia coli bolA is a morphogene involved in stress response and cell division. Overexpression of bolA induces biofilm formation and affects the levels of carboxypeptidases PBP5, PBP6 and β-lactamase AmpC. In this study, we have shown that changes in the expression of bolA result in alterations in the properties of the outer membrane. The sensitivity to detergents and vancomycin was reduced when bolA was overexpressed and fluorescent probes indicated that different levels of bolA had an effect on outer membrane protein accessibility. Moreover, bolA was shown to be involved in the modulation of the OmpF/OmpC balance.
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spelling Effect of the morphogene bolA on the permeability of the Escherichia coli outer membranebolACell morphologyEscherichia coliOuter membranePermeabilityMicrobiologyMolecular BiologyGeneticsEscherichia coli bolA is a morphogene involved in stress response and cell division. Overexpression of bolA induces biofilm formation and affects the levels of carboxypeptidases PBP5, PBP6 and β-lactamase AmpC. In this study, we have shown that changes in the expression of bolA result in alterations in the properties of the outer membrane. The sensitivity to detergents and vancomycin was reduced when bolA was overexpressed and fluorescent probes indicated that different levels of bolA had an effect on outer membrane protein accessibility. Moreover, bolA was shown to be involved in the modulation of the OmpF/OmpC balance.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNFreire, PatrickVieira, Helena L.A.Furtado, Ana R.De Pedro, Miguel A.Arraiano, Cecília M.2023-01-12T22:12:08Z2006-07-012006-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6application/pdfhttp://hdl.handle.net/10362/147414eng0378-1097PURE: 15803428https://doi.org/10.1111/j.1574-6968.2006.00307.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:28:30Zoai:run.unl.pt:10362/147414Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:54.492344Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of the morphogene bolA on the permeability of the Escherichia coli outer membrane
title Effect of the morphogene bolA on the permeability of the Escherichia coli outer membrane
spellingShingle Effect of the morphogene bolA on the permeability of the Escherichia coli outer membrane
Freire, Patrick
bolA
Cell morphology
Escherichia coli
Outer membrane
Permeability
Microbiology
Molecular Biology
Genetics
title_short Effect of the morphogene bolA on the permeability of the Escherichia coli outer membrane
title_full Effect of the morphogene bolA on the permeability of the Escherichia coli outer membrane
title_fullStr Effect of the morphogene bolA on the permeability of the Escherichia coli outer membrane
title_full_unstemmed Effect of the morphogene bolA on the permeability of the Escherichia coli outer membrane
title_sort Effect of the morphogene bolA on the permeability of the Escherichia coli outer membrane
author Freire, Patrick
author_facet Freire, Patrick
Vieira, Helena L.A.
Furtado, Ana R.
De Pedro, Miguel A.
Arraiano, Cecília M.
author_role author
author2 Vieira, Helena L.A.
Furtado, Ana R.
De Pedro, Miguel A.
Arraiano, Cecília M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Freire, Patrick
Vieira, Helena L.A.
Furtado, Ana R.
De Pedro, Miguel A.
Arraiano, Cecília M.
dc.subject.por.fl_str_mv bolA
Cell morphology
Escherichia coli
Outer membrane
Permeability
Microbiology
Molecular Biology
Genetics
topic bolA
Cell morphology
Escherichia coli
Outer membrane
Permeability
Microbiology
Molecular Biology
Genetics
description Escherichia coli bolA is a morphogene involved in stress response and cell division. Overexpression of bolA induces biofilm formation and affects the levels of carboxypeptidases PBP5, PBP6 and β-lactamase AmpC. In this study, we have shown that changes in the expression of bolA result in alterations in the properties of the outer membrane. The sensitivity to detergents and vancomycin was reduced when bolA was overexpressed and fluorescent probes indicated that different levels of bolA had an effect on outer membrane protein accessibility. Moreover, bolA was shown to be involved in the modulation of the OmpF/OmpC balance.
publishDate 2006
dc.date.none.fl_str_mv 2006-07-01
2006-07-01T00:00:00Z
2023-01-12T22:12:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/147414
url http://hdl.handle.net/10362/147414
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0378-1097
PURE: 15803428
https://doi.org/10.1111/j.1574-6968.2006.00307.x
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 6
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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