Cationic agents for DNA compaction
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5003 https://doi.org/10.1016/j.jcis.2008.04.006 |
Resumo: | Fluorescence microscopy was used to investigate the conformational changes of individual T4 DNA molecules induced by different compacting agents, namely the cationic surfactants, cetyltrimethylammonium bromide (CTAB) and chloride (CTAC), iron(III), lysozyme, and protamine sulfate. A protocol for establishing size estimates is suggested to obtain reproducible results. Observations show that in the presence of lysozyme and protamine sulfate, DNA molecules exhibit a conformational change from an elongated coil structure to compact globules, usually interpreted as a first-order transition. The maximum degree of compaction that is attained when iron(III) or CTAB (CTAC) are used as compacting agents is considerably smaller, and intermediate structures (less elongated coils) are visible even for high concentrations of these agents. Dynamic light scattering experiments were carried out, for some of the systems, to assess the reliability of size estimates from fluorescence microscopy. |
id |
RCAP_688add7c7e9aaf939bc211d7309ef822 |
---|---|
oai_identifier_str |
oai:estudogeral.uc.pt:10316/5003 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Cationic agents for DNA compactionDNA compactionCoexistenceIntermediate structuresFluorescence microscopyDynamic light scatteringFluorescence microscopy was used to investigate the conformational changes of individual T4 DNA molecules induced by different compacting agents, namely the cationic surfactants, cetyltrimethylammonium bromide (CTAB) and chloride (CTAC), iron(III), lysozyme, and protamine sulfate. A protocol for establishing size estimates is suggested to obtain reproducible results. Observations show that in the presence of lysozyme and protamine sulfate, DNA molecules exhibit a conformational change from an elongated coil structure to compact globules, usually interpreted as a first-order transition. The maximum degree of compaction that is attained when iron(III) or CTAB (CTAC) are used as compacting agents is considerably smaller, and intermediate structures (less elongated coils) are visible even for high concentrations of these agents. Dynamic light scattering experiments were carried out, for some of the systems, to assess the reliability of size estimates from fluorescence microscopy.http://www.sciencedirect.com/science/article/B6WHR-4S7SV46-1/1/4fec487f09e6c653d0e22a0669f4e0d32008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5003http://hdl.handle.net/10316/5003https://doi.org/10.1016/j.jcis.2008.04.006engJournal of Colloid and Interface Science. 323:1 (2008) 75-83Gaweda, SylwiaMorán, M. CarmenPais, Alberto A. C. C.Dias, Rita S.Schillén, KarinLindman, BjörnMiguel, M. Graçainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-11-06T16:49:09Zoai:estudogeral.uc.pt:10316/5003Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:29.941260Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Cationic agents for DNA compaction |
title |
Cationic agents for DNA compaction |
spellingShingle |
Cationic agents for DNA compaction Gaweda, Sylwia DNA compaction Coexistence Intermediate structures Fluorescence microscopy Dynamic light scattering |
title_short |
Cationic agents for DNA compaction |
title_full |
Cationic agents for DNA compaction |
title_fullStr |
Cationic agents for DNA compaction |
title_full_unstemmed |
Cationic agents for DNA compaction |
title_sort |
Cationic agents for DNA compaction |
author |
Gaweda, Sylwia |
author_facet |
Gaweda, Sylwia Morán, M. Carmen Pais, Alberto A. C. C. Dias, Rita S. Schillén, Karin Lindman, Björn Miguel, M. Graça |
author_role |
author |
author2 |
Morán, M. Carmen Pais, Alberto A. C. C. Dias, Rita S. Schillén, Karin Lindman, Björn Miguel, M. Graça |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Gaweda, Sylwia Morán, M. Carmen Pais, Alberto A. C. C. Dias, Rita S. Schillén, Karin Lindman, Björn Miguel, M. Graça |
dc.subject.por.fl_str_mv |
DNA compaction Coexistence Intermediate structures Fluorescence microscopy Dynamic light scattering |
topic |
DNA compaction Coexistence Intermediate structures Fluorescence microscopy Dynamic light scattering |
description |
Fluorescence microscopy was used to investigate the conformational changes of individual T4 DNA molecules induced by different compacting agents, namely the cationic surfactants, cetyltrimethylammonium bromide (CTAB) and chloride (CTAC), iron(III), lysozyme, and protamine sulfate. A protocol for establishing size estimates is suggested to obtain reproducible results. Observations show that in the presence of lysozyme and protamine sulfate, DNA molecules exhibit a conformational change from an elongated coil structure to compact globules, usually interpreted as a first-order transition. The maximum degree of compaction that is attained when iron(III) or CTAB (CTAC) are used as compacting agents is considerably smaller, and intermediate structures (less elongated coils) are visible even for high concentrations of these agents. Dynamic light scattering experiments were carried out, for some of the systems, to assess the reliability of size estimates from fluorescence microscopy. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5003 http://hdl.handle.net/10316/5003 https://doi.org/10.1016/j.jcis.2008.04.006 |
url |
http://hdl.handle.net/10316/5003 https://doi.org/10.1016/j.jcis.2008.04.006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Colloid and Interface Science. 323:1 (2008) 75-83 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133906361909248 |