Listeria monocytogenes biofilm-associated protein (BapL) may contribute to surface attachment of L-monocytogenes but is absent from many field isolates
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/11922 |
Resumo: | Listeria monocytogenes is a food-borne pathogen capable of adhering to a range of surfaces utilized within the food industry, including stainless steel. The factors required for the attachment of this ubiquitous organism to abiotic surfaces are still relatively unknown. In silico analysis of the L. monocytogenes EGD genome identified a putative cell wall-anchored protein (Lmo0435 [BapL]), which had similarity to proteins involved in biofilm formation by staphylococci. An insertion mutation was constructed in L. monocytogenes to determine the influence of this protein on attachment to abiotic surfaces. The results show that the protein may contribute to the surface adherence of strains that possess BapL, but it is not an essential requirement for all L. monocytogenes strains. Several BapL-negative field isolates demonstrated an ability to adhere to abiotic surfaces equivalent to that of BapL-positive strains. BapL is not required for the virulence of L. monocytogenes in mice. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
spelling |
Listeria monocytogenes biofilm-associated protein (BapL) may contribute to surface attachment of L-monocytogenes but is absent from many field isolatesStaphylococcus-AureusIntercellular-AdhesionFoodborne ListeriosisOutbreakStrainsGrowthIdentificationTemperatureVirulencePlasmidListeria monocytogenes is a food-borne pathogen capable of adhering to a range of surfaces utilized within the food industry, including stainless steel. The factors required for the attachment of this ubiquitous organism to abiotic surfaces are still relatively unknown. In silico analysis of the L. monocytogenes EGD genome identified a putative cell wall-anchored protein (Lmo0435 [BapL]), which had similarity to proteins involved in biofilm formation by staphylococci. An insertion mutation was constructed in L. monocytogenes to determine the influence of this protein on attachment to abiotic surfaces. The results show that the protein may contribute to the surface adherence of strains that possess BapL, but it is not an essential requirement for all L. monocytogenes strains. Several BapL-negative field isolates demonstrated an ability to adhere to abiotic surfaces equivalent to that of BapL-positive strains. BapL is not required for the virulence of L. monocytogenes in mice.European Union; LMTOOCHE consortium [QLKL1-CT-2002-02219]American Society for MicrobiologySapientiaJordan, Suzanne J.Perni, StefanoGlenn, SarahFernandes, IsabelBarbosa, ManuelaSol, ManuelaTenreiro, Rogerio P.Chambel, LeliaBarata, BelarminoZilhao, IsabelAldsworth, Timothy G.Adrião, Andreia Lúcia GonçalvesFaleiro, LShama, GilbertAndrew, Peter W.2018-12-07T14:58:14Z2008-092008-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/11922eng0099-224010.1128/AEM.02419-07info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:23:49Zoai:sapientia.ualg.pt:10400.1/11922Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:03:21.547205Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Listeria monocytogenes biofilm-associated protein (BapL) may contribute to surface attachment of L-monocytogenes but is absent from many field isolates |
title |
Listeria monocytogenes biofilm-associated protein (BapL) may contribute to surface attachment of L-monocytogenes but is absent from many field isolates |
spellingShingle |
Listeria monocytogenes biofilm-associated protein (BapL) may contribute to surface attachment of L-monocytogenes but is absent from many field isolates Jordan, Suzanne J. Staphylococcus-Aureus Intercellular-Adhesion Foodborne Listeriosis Outbreak Strains Growth Identification Temperature Virulence Plasmid |
title_short |
Listeria monocytogenes biofilm-associated protein (BapL) may contribute to surface attachment of L-monocytogenes but is absent from many field isolates |
title_full |
Listeria monocytogenes biofilm-associated protein (BapL) may contribute to surface attachment of L-monocytogenes but is absent from many field isolates |
title_fullStr |
Listeria monocytogenes biofilm-associated protein (BapL) may contribute to surface attachment of L-monocytogenes but is absent from many field isolates |
title_full_unstemmed |
Listeria monocytogenes biofilm-associated protein (BapL) may contribute to surface attachment of L-monocytogenes but is absent from many field isolates |
title_sort |
Listeria monocytogenes biofilm-associated protein (BapL) may contribute to surface attachment of L-monocytogenes but is absent from many field isolates |
author |
Jordan, Suzanne J. |
author_facet |
Jordan, Suzanne J. Perni, Stefano Glenn, Sarah Fernandes, Isabel Barbosa, Manuela Sol, Manuela Tenreiro, Rogerio P. Chambel, Lelia Barata, Belarmino Zilhao, Isabel Aldsworth, Timothy G. Adrião, Andreia Lúcia Gonçalves Faleiro, L Shama, Gilbert Andrew, Peter W. |
author_role |
author |
author2 |
Perni, Stefano Glenn, Sarah Fernandes, Isabel Barbosa, Manuela Sol, Manuela Tenreiro, Rogerio P. Chambel, Lelia Barata, Belarmino Zilhao, Isabel Aldsworth, Timothy G. Adrião, Andreia Lúcia Gonçalves Faleiro, L Shama, Gilbert Andrew, Peter W. |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Jordan, Suzanne J. Perni, Stefano Glenn, Sarah Fernandes, Isabel Barbosa, Manuela Sol, Manuela Tenreiro, Rogerio P. Chambel, Lelia Barata, Belarmino Zilhao, Isabel Aldsworth, Timothy G. Adrião, Andreia Lúcia Gonçalves Faleiro, L Shama, Gilbert Andrew, Peter W. |
dc.subject.por.fl_str_mv |
Staphylococcus-Aureus Intercellular-Adhesion Foodborne Listeriosis Outbreak Strains Growth Identification Temperature Virulence Plasmid |
topic |
Staphylococcus-Aureus Intercellular-Adhesion Foodborne Listeriosis Outbreak Strains Growth Identification Temperature Virulence Plasmid |
description |
Listeria monocytogenes is a food-borne pathogen capable of adhering to a range of surfaces utilized within the food industry, including stainless steel. The factors required for the attachment of this ubiquitous organism to abiotic surfaces are still relatively unknown. In silico analysis of the L. monocytogenes EGD genome identified a putative cell wall-anchored protein (Lmo0435 [BapL]), which had similarity to proteins involved in biofilm formation by staphylococci. An insertion mutation was constructed in L. monocytogenes to determine the influence of this protein on attachment to abiotic surfaces. The results show that the protein may contribute to the surface adherence of strains that possess BapL, but it is not an essential requirement for all L. monocytogenes strains. Several BapL-negative field isolates demonstrated an ability to adhere to abiotic surfaces equivalent to that of BapL-positive strains. BapL is not required for the virulence of L. monocytogenes in mice. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-09 2008-09-01T00:00:00Z 2018-12-07T14:58:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/11922 |
url |
http://hdl.handle.net/10400.1/11922 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0099-2240 10.1128/AEM.02419-07 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133267959480320 |