Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife

Detalhes bibliográficos
Autor(a) principal: Lyashchenko, K. P.
Data de Publicação: 2008
Outros Autores: Greenwald, R., Esfandiari, J., Chambers, M. A., Vicente, J., Gortazar, C., Santos, N., Correia-Neves, M, Buddle, B. M., Jackson, R., O'Brien, D. J., Schmitt, S., Palmer, M. V., Delahay, R. J., Waters, W. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/61478
Resumo: Numerous species of mammals are susceptible to Mycobacterium bovis, the causative agent of bovine tuberculosis (TB). Several wildlife hosts have emerged as reservoirs of M. bovis infection for domestic livestock in different countries. In the present study, blood samples were collected from Eurasian badgers (n=1532), white-tailed deer (n=463), brushtail possums (n=129), and wild boar (n=177) for evaluation of antibody responses to M. bovis infection by a lateral-flow rapid test (RT) and multiantigen print immunoassay (MAPIA). Magnitude of the antibody responses and antigen recognition patterns varied among the animals as determined by MAPIA; however, MPB83 was the most commonly recognized antigen for each host studied. Other seroreactive antigens included ESAT-6, CFP10, and MPB70. The agreement of the RT with culture results varied from 74% for possums to 81% for badgers to 90% for wild boar to 97% for white-tailed deer. Small numbers of wild boar and deer exposed to M. avium infection or paratuberculosis, respectively, did not cross-react in the RT, supporting the high specificity of the assay. In deer, whole blood samples reacted similarly to corresponding serum specimens (97% concordance), demonstrating the potential for field application. As previously demonstrated for badgers and deer, antibody responses to M. bovis infection in wild boar were positively associated with advanced disease. Together, these findings suggest that a rapid TB assay such as the RT may provide a useful screening tool for certain wildlife species that may be implicated in the maintenance and transmission of M. bovis infection to domestic livestock.
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spelling Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlifeAnimalsAnimals, WildAntibodies, BacterialAntigens, BacterialCattleDeerMustelidaeMycobacterium bovisNew ZealandPortugalSerologic TestsSpainSus scrofaTrichosurusTuberculosis, BovineUnited KingdomUnited StatesTuberculosisWildlifeSerologyCiências Médicas::Outras Ciências MédicasScience & TechnologyNumerous species of mammals are susceptible to Mycobacterium bovis, the causative agent of bovine tuberculosis (TB). Several wildlife hosts have emerged as reservoirs of M. bovis infection for domestic livestock in different countries. In the present study, blood samples were collected from Eurasian badgers (n=1532), white-tailed deer (n=463), brushtail possums (n=129), and wild boar (n=177) for evaluation of antibody responses to M. bovis infection by a lateral-flow rapid test (RT) and multiantigen print immunoassay (MAPIA). Magnitude of the antibody responses and antigen recognition patterns varied among the animals as determined by MAPIA; however, MPB83 was the most commonly recognized antigen for each host studied. Other seroreactive antigens included ESAT-6, CFP10, and MPB70. The agreement of the RT with culture results varied from 74% for possums to 81% for badgers to 90% for wild boar to 97% for white-tailed deer. Small numbers of wild boar and deer exposed to M. avium infection or paratuberculosis, respectively, did not cross-react in the RT, supporting the high specificity of the assay. In deer, whole blood samples reacted similarly to corresponding serum specimens (97% concordance), demonstrating the potential for field application. As previously demonstrated for badgers and deer, antibody responses to M. bovis infection in wild boar were positively associated with advanced disease. Together, these findings suggest that a rapid TB assay such as the RT may provide a useful screening tool for certain wildlife species that may be implicated in the maintenance and transmission of M. bovis infection to domestic livestock.The authors are grateful to Peter Andersen and Jim McNair for kindly providing certain antigens used in C this study. Badger samples were taken under projects funded by the Department for Environment, Food, and Rural Affairs (Defra), UK. The authors acknowledge the support of staff from CSL, VLA Starcross, Defra Wildlife Unit, and permission from the Independent Scientific Group for use of sera from the RBCT. Spanish wild boar samples were obtained with support from MEC Plan Nacional AGL2005-07401 and Santander - Fundacion M. Botin.ElsevierUniversidade do MinhoLyashchenko, K. P.Greenwald, R.Esfandiari, J.Chambers, M. A.Vicente, J.Gortazar, C.Santos, N.Correia-Neves, MBuddle, B. M.Jackson, R.O'Brien, D. J.Schmitt, S.Palmer, M. V.Delahay, R. J.Waters, W. R.2008-12-102008-12-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/61478engLyashchenko, K. P., Greenwald, R., Esfandiari, J., et. al.(2008). Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife. Veterinary microbiology, 132(3-4), 283-2920378-11351873-254210.1016/j.vetmic.2008.05.02918602770https://www.sciencedirect.com/science/article/pii/S0378113508002046info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:24:06Zoai:repositorium.sdum.uminho.pt:1822/61478Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:18:00.202687Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife
title Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife
spellingShingle Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife
Lyashchenko, K. P.
Animals
Animals, Wild
Antibodies, Bacterial
Antigens, Bacterial
Cattle
Deer
Mustelidae
Mycobacterium bovis
New Zealand
Portugal
Serologic Tests
Spain
Sus scrofa
Trichosurus
Tuberculosis, Bovine
United Kingdom
United States
Tuberculosis
Wildlife
Serology
Ciências Médicas::Outras Ciências Médicas
Science & Technology
title_short Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife
title_full Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife
title_fullStr Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife
title_full_unstemmed Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife
title_sort Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife
author Lyashchenko, K. P.
author_facet Lyashchenko, K. P.
Greenwald, R.
Esfandiari, J.
Chambers, M. A.
Vicente, J.
Gortazar, C.
Santos, N.
Correia-Neves, M
Buddle, B. M.
Jackson, R.
O'Brien, D. J.
Schmitt, S.
Palmer, M. V.
Delahay, R. J.
Waters, W. R.
author_role author
author2 Greenwald, R.
Esfandiari, J.
Chambers, M. A.
Vicente, J.
Gortazar, C.
Santos, N.
Correia-Neves, M
Buddle, B. M.
Jackson, R.
O'Brien, D. J.
Schmitt, S.
Palmer, M. V.
Delahay, R. J.
Waters, W. R.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Lyashchenko, K. P.
Greenwald, R.
Esfandiari, J.
Chambers, M. A.
Vicente, J.
Gortazar, C.
Santos, N.
Correia-Neves, M
Buddle, B. M.
Jackson, R.
O'Brien, D. J.
Schmitt, S.
Palmer, M. V.
Delahay, R. J.
Waters, W. R.
dc.subject.por.fl_str_mv Animals
Animals, Wild
Antibodies, Bacterial
Antigens, Bacterial
Cattle
Deer
Mustelidae
Mycobacterium bovis
New Zealand
Portugal
Serologic Tests
Spain
Sus scrofa
Trichosurus
Tuberculosis, Bovine
United Kingdom
United States
Tuberculosis
Wildlife
Serology
Ciências Médicas::Outras Ciências Médicas
Science & Technology
topic Animals
Animals, Wild
Antibodies, Bacterial
Antigens, Bacterial
Cattle
Deer
Mustelidae
Mycobacterium bovis
New Zealand
Portugal
Serologic Tests
Spain
Sus scrofa
Trichosurus
Tuberculosis, Bovine
United Kingdom
United States
Tuberculosis
Wildlife
Serology
Ciências Médicas::Outras Ciências Médicas
Science & Technology
description Numerous species of mammals are susceptible to Mycobacterium bovis, the causative agent of bovine tuberculosis (TB). Several wildlife hosts have emerged as reservoirs of M. bovis infection for domestic livestock in different countries. In the present study, blood samples were collected from Eurasian badgers (n=1532), white-tailed deer (n=463), brushtail possums (n=129), and wild boar (n=177) for evaluation of antibody responses to M. bovis infection by a lateral-flow rapid test (RT) and multiantigen print immunoassay (MAPIA). Magnitude of the antibody responses and antigen recognition patterns varied among the animals as determined by MAPIA; however, MPB83 was the most commonly recognized antigen for each host studied. Other seroreactive antigens included ESAT-6, CFP10, and MPB70. The agreement of the RT with culture results varied from 74% for possums to 81% for badgers to 90% for wild boar to 97% for white-tailed deer. Small numbers of wild boar and deer exposed to M. avium infection or paratuberculosis, respectively, did not cross-react in the RT, supporting the high specificity of the assay. In deer, whole blood samples reacted similarly to corresponding serum specimens (97% concordance), demonstrating the potential for field application. As previously demonstrated for badgers and deer, antibody responses to M. bovis infection in wild boar were positively associated with advanced disease. Together, these findings suggest that a rapid TB assay such as the RT may provide a useful screening tool for certain wildlife species that may be implicated in the maintenance and transmission of M. bovis infection to domestic livestock.
publishDate 2008
dc.date.none.fl_str_mv 2008-12-10
2008-12-10T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/61478
url http://hdl.handle.net/1822/61478
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lyashchenko, K. P., Greenwald, R., Esfandiari, J., et. al.(2008). Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife. Veterinary microbiology, 132(3-4), 283-292
0378-1135
1873-2542
10.1016/j.vetmic.2008.05.029
18602770
https://www.sciencedirect.com/science/article/pii/S0378113508002046
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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