Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/61478 |
Resumo: | Numerous species of mammals are susceptible to Mycobacterium bovis, the causative agent of bovine tuberculosis (TB). Several wildlife hosts have emerged as reservoirs of M. bovis infection for domestic livestock in different countries. In the present study, blood samples were collected from Eurasian badgers (n=1532), white-tailed deer (n=463), brushtail possums (n=129), and wild boar (n=177) for evaluation of antibody responses to M. bovis infection by a lateral-flow rapid test (RT) and multiantigen print immunoassay (MAPIA). Magnitude of the antibody responses and antigen recognition patterns varied among the animals as determined by MAPIA; however, MPB83 was the most commonly recognized antigen for each host studied. Other seroreactive antigens included ESAT-6, CFP10, and MPB70. The agreement of the RT with culture results varied from 74% for possums to 81% for badgers to 90% for wild boar to 97% for white-tailed deer. Small numbers of wild boar and deer exposed to M. avium infection or paratuberculosis, respectively, did not cross-react in the RT, supporting the high specificity of the assay. In deer, whole blood samples reacted similarly to corresponding serum specimens (97% concordance), demonstrating the potential for field application. As previously demonstrated for badgers and deer, antibody responses to M. bovis infection in wild boar were positively associated with advanced disease. Together, these findings suggest that a rapid TB assay such as the RT may provide a useful screening tool for certain wildlife species that may be implicated in the maintenance and transmission of M. bovis infection to domestic livestock. |
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Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlifeAnimalsAnimals, WildAntibodies, BacterialAntigens, BacterialCattleDeerMustelidaeMycobacterium bovisNew ZealandPortugalSerologic TestsSpainSus scrofaTrichosurusTuberculosis, BovineUnited KingdomUnited StatesTuberculosisWildlifeSerologyCiências Médicas::Outras Ciências MédicasScience & TechnologyNumerous species of mammals are susceptible to Mycobacterium bovis, the causative agent of bovine tuberculosis (TB). Several wildlife hosts have emerged as reservoirs of M. bovis infection for domestic livestock in different countries. In the present study, blood samples were collected from Eurasian badgers (n=1532), white-tailed deer (n=463), brushtail possums (n=129), and wild boar (n=177) for evaluation of antibody responses to M. bovis infection by a lateral-flow rapid test (RT) and multiantigen print immunoassay (MAPIA). Magnitude of the antibody responses and antigen recognition patterns varied among the animals as determined by MAPIA; however, MPB83 was the most commonly recognized antigen for each host studied. Other seroreactive antigens included ESAT-6, CFP10, and MPB70. The agreement of the RT with culture results varied from 74% for possums to 81% for badgers to 90% for wild boar to 97% for white-tailed deer. Small numbers of wild boar and deer exposed to M. avium infection or paratuberculosis, respectively, did not cross-react in the RT, supporting the high specificity of the assay. In deer, whole blood samples reacted similarly to corresponding serum specimens (97% concordance), demonstrating the potential for field application. As previously demonstrated for badgers and deer, antibody responses to M. bovis infection in wild boar were positively associated with advanced disease. Together, these findings suggest that a rapid TB assay such as the RT may provide a useful screening tool for certain wildlife species that may be implicated in the maintenance and transmission of M. bovis infection to domestic livestock.The authors are grateful to Peter Andersen and Jim McNair for kindly providing certain antigens used in C this study. Badger samples were taken under projects funded by the Department for Environment, Food, and Rural Affairs (Defra), UK. The authors acknowledge the support of staff from CSL, VLA Starcross, Defra Wildlife Unit, and permission from the Independent Scientific Group for use of sera from the RBCT. Spanish wild boar samples were obtained with support from MEC Plan Nacional AGL2005-07401 and Santander - Fundacion M. Botin.ElsevierUniversidade do MinhoLyashchenko, K. P.Greenwald, R.Esfandiari, J.Chambers, M. A.Vicente, J.Gortazar, C.Santos, N.Correia-Neves, MBuddle, B. M.Jackson, R.O'Brien, D. J.Schmitt, S.Palmer, M. V.Delahay, R. J.Waters, W. R.2008-12-102008-12-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/61478engLyashchenko, K. P., Greenwald, R., Esfandiari, J., et. al.(2008). Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife. Veterinary microbiology, 132(3-4), 283-2920378-11351873-254210.1016/j.vetmic.2008.05.02918602770https://www.sciencedirect.com/science/article/pii/S0378113508002046info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:24:06Zoai:repositorium.sdum.uminho.pt:1822/61478Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:18:00.202687Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife |
title |
Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife |
spellingShingle |
Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife Lyashchenko, K. P. Animals Animals, Wild Antibodies, Bacterial Antigens, Bacterial Cattle Deer Mustelidae Mycobacterium bovis New Zealand Portugal Serologic Tests Spain Sus scrofa Trichosurus Tuberculosis, Bovine United Kingdom United States Tuberculosis Wildlife Serology Ciências Médicas::Outras Ciências Médicas Science & Technology |
title_short |
Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife |
title_full |
Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife |
title_fullStr |
Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife |
title_full_unstemmed |
Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife |
title_sort |
Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife |
author |
Lyashchenko, K. P. |
author_facet |
Lyashchenko, K. P. Greenwald, R. Esfandiari, J. Chambers, M. A. Vicente, J. Gortazar, C. Santos, N. Correia-Neves, M Buddle, B. M. Jackson, R. O'Brien, D. J. Schmitt, S. Palmer, M. V. Delahay, R. J. Waters, W. R. |
author_role |
author |
author2 |
Greenwald, R. Esfandiari, J. Chambers, M. A. Vicente, J. Gortazar, C. Santos, N. Correia-Neves, M Buddle, B. M. Jackson, R. O'Brien, D. J. Schmitt, S. Palmer, M. V. Delahay, R. J. Waters, W. R. |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Lyashchenko, K. P. Greenwald, R. Esfandiari, J. Chambers, M. A. Vicente, J. Gortazar, C. Santos, N. Correia-Neves, M Buddle, B. M. Jackson, R. O'Brien, D. J. Schmitt, S. Palmer, M. V. Delahay, R. J. Waters, W. R. |
dc.subject.por.fl_str_mv |
Animals Animals, Wild Antibodies, Bacterial Antigens, Bacterial Cattle Deer Mustelidae Mycobacterium bovis New Zealand Portugal Serologic Tests Spain Sus scrofa Trichosurus Tuberculosis, Bovine United Kingdom United States Tuberculosis Wildlife Serology Ciências Médicas::Outras Ciências Médicas Science & Technology |
topic |
Animals Animals, Wild Antibodies, Bacterial Antigens, Bacterial Cattle Deer Mustelidae Mycobacterium bovis New Zealand Portugal Serologic Tests Spain Sus scrofa Trichosurus Tuberculosis, Bovine United Kingdom United States Tuberculosis Wildlife Serology Ciências Médicas::Outras Ciências Médicas Science & Technology |
description |
Numerous species of mammals are susceptible to Mycobacterium bovis, the causative agent of bovine tuberculosis (TB). Several wildlife hosts have emerged as reservoirs of M. bovis infection for domestic livestock in different countries. In the present study, blood samples were collected from Eurasian badgers (n=1532), white-tailed deer (n=463), brushtail possums (n=129), and wild boar (n=177) for evaluation of antibody responses to M. bovis infection by a lateral-flow rapid test (RT) and multiantigen print immunoassay (MAPIA). Magnitude of the antibody responses and antigen recognition patterns varied among the animals as determined by MAPIA; however, MPB83 was the most commonly recognized antigen for each host studied. Other seroreactive antigens included ESAT-6, CFP10, and MPB70. The agreement of the RT with culture results varied from 74% for possums to 81% for badgers to 90% for wild boar to 97% for white-tailed deer. Small numbers of wild boar and deer exposed to M. avium infection or paratuberculosis, respectively, did not cross-react in the RT, supporting the high specificity of the assay. In deer, whole blood samples reacted similarly to corresponding serum specimens (97% concordance), demonstrating the potential for field application. As previously demonstrated for badgers and deer, antibody responses to M. bovis infection in wild boar were positively associated with advanced disease. Together, these findings suggest that a rapid TB assay such as the RT may provide a useful screening tool for certain wildlife species that may be implicated in the maintenance and transmission of M. bovis infection to domestic livestock. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-12-10 2008-12-10T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/61478 |
url |
http://hdl.handle.net/1822/61478 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lyashchenko, K. P., Greenwald, R., Esfandiari, J., et. al.(2008). Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife. Veterinary microbiology, 132(3-4), 283-292 0378-1135 1873-2542 10.1016/j.vetmic.2008.05.029 18602770 https://www.sciencedirect.com/science/article/pii/S0378113508002046 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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