Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.8/8260 |
Resumo: | Gene therapy could be simply defined as a strategy for the introduction of a functional copy of desired genes in patients, to correct some specific mutation and potentially treat the respective disorder. However, this straightforward definition hides very complex processes related to the design and preparation of the therapeutic genes, as well as the development of suitable gene delivery systems. Within non-viral vectors, polymeric nanocarriers have offered an ideal platform to be applied as gene delivery systems. Concerning this, the main goal of the study was to do a systematic evaluation on the formulation of pDNA delivery systems based on the complexation of different sized plasmids with chitosan (CH) or polyethyleneimine (PEI) polymers to search for the best option regarding encapsulation efficiency, surface charge, size, and delivery ability. The cytotoxicity and the transfection efficiency of these systems were accessed and, for the best p53 encoding pDNA nanosystems, the ability to promote protein expression was also evaluated. Overall, it was showed that CH polyplexes are more efficient on transfection when compared with the PEI polyplexes, resulting in higher P53 protein expression. Cells transfected with CH/p53-pDNA polyplexes presented an increase of around 54.2% on P53 expression, while the transfection with the PEI/p53-pDNA polyplexes resulted in a 32% increase. |
id |
RCAP_6952b53d382b6d6d9076a91fc9587124 |
---|---|
oai_identifier_str |
oai:iconline.ipleiria.pt:10400.8/8260 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes FormulationTransgene expressionPolyplexesPolyethyleneimineGene therapyChitosanGene therapy could be simply defined as a strategy for the introduction of a functional copy of desired genes in patients, to correct some specific mutation and potentially treat the respective disorder. However, this straightforward definition hides very complex processes related to the design and preparation of the therapeutic genes, as well as the development of suitable gene delivery systems. Within non-viral vectors, polymeric nanocarriers have offered an ideal platform to be applied as gene delivery systems. Concerning this, the main goal of the study was to do a systematic evaluation on the formulation of pDNA delivery systems based on the complexation of different sized plasmids with chitosan (CH) or polyethyleneimine (PEI) polymers to search for the best option regarding encapsulation efficiency, surface charge, size, and delivery ability. The cytotoxicity and the transfection efficiency of these systems were accessed and, for the best p53 encoding pDNA nanosystems, the ability to promote protein expression was also evaluated. Overall, it was showed that CH polyplexes are more efficient on transfection when compared with the PEI polyplexes, resulting in higher P53 protein expression. Cells transfected with CH/p53-pDNA polyplexes presented an increase of around 54.2% on P53 expression, while the transfection with the PEI/p53-pDNA polyplexes resulted in a 32% increase.MDPIIC-OnlineValente, J. F. A.Pereira, P.Sousa, A.Queiroz, J. A.Sousa, F.2023-03-20T11:45:35Z2021-03-052021-03-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/8260engValente, J. F. A., Pereira, P., Sousa, A., Queiroz, J. A., & Sousa, F. (2021). Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation. Polymers, 13(5), 793. MDPI AG. Retrieved from http://dx.doi.org/10.3390/polym1305079310.3390/polym13050793info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:56:57Zoai:iconline.ipleiria.pt:10400.8/8260Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:51:01.629226Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation |
title |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation |
spellingShingle |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation Valente, J. F. A. Transgene expression Polyplexes Polyethyleneimine Gene therapy Chitosan |
title_short |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation |
title_full |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation |
title_fullStr |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation |
title_full_unstemmed |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation |
title_sort |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation |
author |
Valente, J. F. A. |
author_facet |
Valente, J. F. A. Pereira, P. Sousa, A. Queiroz, J. A. Sousa, F. |
author_role |
author |
author2 |
Pereira, P. Sousa, A. Queiroz, J. A. Sousa, F. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
IC-Online |
dc.contributor.author.fl_str_mv |
Valente, J. F. A. Pereira, P. Sousa, A. Queiroz, J. A. Sousa, F. |
dc.subject.por.fl_str_mv |
Transgene expression Polyplexes Polyethyleneimine Gene therapy Chitosan |
topic |
Transgene expression Polyplexes Polyethyleneimine Gene therapy Chitosan |
description |
Gene therapy could be simply defined as a strategy for the introduction of a functional copy of desired genes in patients, to correct some specific mutation and potentially treat the respective disorder. However, this straightforward definition hides very complex processes related to the design and preparation of the therapeutic genes, as well as the development of suitable gene delivery systems. Within non-viral vectors, polymeric nanocarriers have offered an ideal platform to be applied as gene delivery systems. Concerning this, the main goal of the study was to do a systematic evaluation on the formulation of pDNA delivery systems based on the complexation of different sized plasmids with chitosan (CH) or polyethyleneimine (PEI) polymers to search for the best option regarding encapsulation efficiency, surface charge, size, and delivery ability. The cytotoxicity and the transfection efficiency of these systems were accessed and, for the best p53 encoding pDNA nanosystems, the ability to promote protein expression was also evaluated. Overall, it was showed that CH polyplexes are more efficient on transfection when compared with the PEI polyplexes, resulting in higher P53 protein expression. Cells transfected with CH/p53-pDNA polyplexes presented an increase of around 54.2% on P53 expression, while the transfection with the PEI/p53-pDNA polyplexes resulted in a 32% increase. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03-05 2021-03-05T00:00:00Z 2023-03-20T11:45:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.8/8260 |
url |
http://hdl.handle.net/10400.8/8260 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Valente, J. F. A., Pereira, P., Sousa, A., Queiroz, J. A., & Sousa, F. (2021). Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation. Polymers, 13(5), 793. MDPI AG. Retrieved from http://dx.doi.org/10.3390/polym13050793 10.3390/polym13050793 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799137002225926144 |