Poly(D,L-lactic acid) scaffolds as an innovative approach to the treatment of mixed S. aureus-C. albicans biofilms

Detalhes bibliográficos
Autor(a) principal: Zegre, Miguel
Data de Publicação: 2023
Outros Autores: Barros, J., Ribeiro, I. A., Santos, C., Aranha Caetano, Liliana, Gonçalves, L., Monteiro, F., Ferraz, M., Bettencourt, A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/16966
Resumo: The authors thank the Fundação para a Ciência e Tecnologia (FCT), Portugal for the financial support: projects UIDB/04138/2020 and UIDP/04138/2020 (iMed.Ulisboa), UIDB/00100/2020 (CQE), L. Gonçalves Principal Researcher grant (CEECIND/03143/2017), UIDB/ 05608/2020 and UIDP/05608/2020 (H&TRC).
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spelling Poly(D,L-lactic acid) scaffolds as an innovative approach to the treatment of mixed S. aureus-C. albicans biofilmsBone infectionMinocyclineVoriconazoleFCT_UIDB/ 05608/2020FCT_UIDP/05608/2020The authors thank the Fundação para a Ciência e Tecnologia (FCT), Portugal for the financial support: projects UIDB/04138/2020 and UIDP/04138/2020 (iMed.Ulisboa), UIDB/00100/2020 (CQE), L. Gonçalves Principal Researcher grant (CEECIND/03143/2017), UIDB/ 05608/2020 and UIDP/05608/2020 (H&TRC).Introduction: The treatment of bacterial joint and bone infections in patients after multiple revision arthroplasties is very challenging. An expanding number of studies report the co-isolation of fungal and bacterial species (such as Candida albicans and Staphylococcus aureus) from polymicrobial biofilm associated with infections related to bone infections. Current investigations establish that local-specific drug delivery scaffolds with low toxicity and increased efficiency to specific sites when compared to oral and systemic administration approaches, can considerably lower the number of viable microorganisms in polymicrobial biofilms, preventing simultaneously the progression of infection in bone disorder. Notably, the development of co-delivery systems of at least two antimicrobials is yet a neglected approach, while it may be a critical strategy for the treatment of infections associated with polymicrobial biofilms. Simultaneously, it is recommended to assess the contribution of each microbial population within the biofilm to select the best therapy to treat polymicrobial infections. Among different biomaterials used in scaffolds as drug-delivery carriers, poly(lactic acid) (PLA) based polymers are being widely studied due to their versatility, low toxicity, and tailored biodegradability having the US Food and Drug Administration approval for clinical use. The adequate osteoconductive and anti-S. aureus effects of a collagen functionalized poly(D, L-lactic acid) (PDLLA) porous scaffold loaded with minocycline (a tetracycline antibiotic) have been previously demonstrated3. In the present study, we focus on the problem of mixed bacterial-fungal biofilm infections and the joining of two antimicrobials in the PDLLA scaffold. Minocycline and voriconazole (an antifungal triazole) were the chosen model drugs, since minocycline may represent a promising drug that can be administered in combination with azoles (namely voriconazole) to treat infections caused by pathogenic Candida species. Morphological and chemical properties of the co-delivery PDLLA scaffolds, as well as drug release profiles, were examined. The antibiofilm activity of these drug delivery systems was tested against single- and dual-species biofilms of S. aureus and C. albicans. The formation of dual-species S. aureus – C. albicans biofilms was studied over time to understand the relationship between both microorganisms during in vitro biofilm formation. Cytocompatibility and osteoconductive tests were also conducted using MG-63 osteoblasts to assess the biocompatibility of the PDLLA scaffolds.Ordem dos FarmacêuuticosRCIPLZegre, MiguelBarros, J.Ribeiro, I. A.Santos, C.Aranha Caetano, LilianaGonçalves, L.Monteiro, F.Ferraz, M.Bettencourt, A.2024-01-23T10:44:12Z2023-102023-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/16966engZegre M, Barros J, Ribeiro IA, Santos C, Caetano LA, Gonçalves L, et al. Poly(D,L-lactic acid) scaffolds as an innovative approach to the treatment of mixed S. Aureus-C. Albicans biofilms. Acta Farm Port. 2023;(Suppl.):29-32.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-31T02:16:08Zoai:repositorio.ipl.pt:10400.21/16966Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:56:53.200182Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Poly(D,L-lactic acid) scaffolds as an innovative approach to the treatment of mixed S. aureus-C. albicans biofilms
title Poly(D,L-lactic acid) scaffolds as an innovative approach to the treatment of mixed S. aureus-C. albicans biofilms
spellingShingle Poly(D,L-lactic acid) scaffolds as an innovative approach to the treatment of mixed S. aureus-C. albicans biofilms
Zegre, Miguel
Bone infection
Minocycline
Voriconazole
FCT_UIDB/ 05608/2020
FCT_UIDP/05608/2020
title_short Poly(D,L-lactic acid) scaffolds as an innovative approach to the treatment of mixed S. aureus-C. albicans biofilms
title_full Poly(D,L-lactic acid) scaffolds as an innovative approach to the treatment of mixed S. aureus-C. albicans biofilms
title_fullStr Poly(D,L-lactic acid) scaffolds as an innovative approach to the treatment of mixed S. aureus-C. albicans biofilms
title_full_unstemmed Poly(D,L-lactic acid) scaffolds as an innovative approach to the treatment of mixed S. aureus-C. albicans biofilms
title_sort Poly(D,L-lactic acid) scaffolds as an innovative approach to the treatment of mixed S. aureus-C. albicans biofilms
author Zegre, Miguel
author_facet Zegre, Miguel
Barros, J.
Ribeiro, I. A.
Santos, C.
Aranha Caetano, Liliana
Gonçalves, L.
Monteiro, F.
Ferraz, M.
Bettencourt, A.
author_role author
author2 Barros, J.
Ribeiro, I. A.
Santos, C.
Aranha Caetano, Liliana
Gonçalves, L.
Monteiro, F.
Ferraz, M.
Bettencourt, A.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Zegre, Miguel
Barros, J.
Ribeiro, I. A.
Santos, C.
Aranha Caetano, Liliana
Gonçalves, L.
Monteiro, F.
Ferraz, M.
Bettencourt, A.
dc.subject.por.fl_str_mv Bone infection
Minocycline
Voriconazole
FCT_UIDB/ 05608/2020
FCT_UIDP/05608/2020
topic Bone infection
Minocycline
Voriconazole
FCT_UIDB/ 05608/2020
FCT_UIDP/05608/2020
description The authors thank the Fundação para a Ciência e Tecnologia (FCT), Portugal for the financial support: projects UIDB/04138/2020 and UIDP/04138/2020 (iMed.Ulisboa), UIDB/00100/2020 (CQE), L. Gonçalves Principal Researcher grant (CEECIND/03143/2017), UIDB/ 05608/2020 and UIDP/05608/2020 (H&TRC).
publishDate 2023
dc.date.none.fl_str_mv 2023-10
2023-10-01T00:00:00Z
2024-01-23T10:44:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/16966
url http://hdl.handle.net/10400.21/16966
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Zegre M, Barros J, Ribeiro IA, Santos C, Caetano LA, Gonçalves L, et al. Poly(D,L-lactic acid) scaffolds as an innovative approach to the treatment of mixed S. Aureus-C. Albicans biofilms. Acta Farm Port. 2023;(Suppl.):29-32.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Ordem dos Farmacêuuticos
publisher.none.fl_str_mv Ordem dos Farmacêuuticos
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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