Isolation and characterization of cgchi3, a nodule-specific gene from Casuarina glauca encoding a class III chitinase

Detalhes bibliográficos
Autor(a) principal: Fortunato, Ana
Data de Publicação: 2007
Outros Autores: Santos, Patricia, Graça, Inês, Gouveia, Maria Manuela, Martins, Sandra Marina, Ricardo, Cândido Pereira Pinto, Pawlowski, Katharina, Ribeiro, Ana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.13/5283
Resumo: Chitinases (EC 3.2.1.14) catalyse the hydrolysis of chitin, a homopolymer of b-1,4-linked N-acetyl-D-glucosamine residues. Plant chitinases are involved in a wide variety of processes; in particular, their expression has been found to be enhanced in symbiotic and pathogenic plant–microbe interactions. During this work we have cloned and characterized a gene encoding a class III chitinase from actinorhizal nodules of Casuarina glauca (cgchi3). CGCHI3 was found to be encoded by a single gene that was specifically activated in nodules as compared with uninoculated control roots and leaves. The expression of this gene was further enhanced in nodules after salicylic acid treatment and completely repressed after wounding. In situ hybridisation analysis revealed that cgchi3 is an early nodulin gene, being expressed in the meristem and in the uninfected cortical cells of young nodules. Based on the obtained results we suggest that this gene is involved in nodule development. This is the first report on a class III chitinase coding gene that is specifically activated during actinorhizal symbiosis.
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spelling Isolation and characterization of cgchi3, a nodule-specific gene from Casuarina glauca encoding a class III chitinasecgchi3Casuarina glaucaChitinase.Faculdade de Ciências da VidaChitinases (EC 3.2.1.14) catalyse the hydrolysis of chitin, a homopolymer of b-1,4-linked N-acetyl-D-glucosamine residues. Plant chitinases are involved in a wide variety of processes; in particular, their expression has been found to be enhanced in symbiotic and pathogenic plant–microbe interactions. During this work we have cloned and characterized a gene encoding a class III chitinase from actinorhizal nodules of Casuarina glauca (cgchi3). CGCHI3 was found to be encoded by a single gene that was specifically activated in nodules as compared with uninoculated control roots and leaves. The expression of this gene was further enhanced in nodules after salicylic acid treatment and completely repressed after wounding. In situ hybridisation analysis revealed that cgchi3 is an early nodulin gene, being expressed in the meristem and in the uninfected cortical cells of young nodules. Based on the obtained results we suggest that this gene is involved in nodule development. This is the first report on a class III chitinase coding gene that is specifically activated during actinorhizal symbiosis.WileyDigitUMaFortunato, AnaSantos, PatriciaGraça, InêsGouveia, Maria ManuelaMartins, Sandra MarinaRicardo, Cândido Pereira PintoPawlowski, KatharinaRibeiro, Ana2023-07-18T15:19:33Z20072007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/5283engFortunato, A., Santos, P., Graça, I., Gouveia, M. M., Martins, S. M., Pinto Ricardo, C. P., ... & Ribeiro, A. (2007). Isolation and characterization of cgchi3, a nodule‐specific gene from Casuarina glauca encoding a class III chitinase. Physiologia Plantarum, 130(3), 418-426.10.1111/j.1399-3054.2006.00864.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-23T03:37:21Zoai:digituma.uma.pt:10400.13/5283Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:54:28.213479Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Isolation and characterization of cgchi3, a nodule-specific gene from Casuarina glauca encoding a class III chitinase
title Isolation and characterization of cgchi3, a nodule-specific gene from Casuarina glauca encoding a class III chitinase
spellingShingle Isolation and characterization of cgchi3, a nodule-specific gene from Casuarina glauca encoding a class III chitinase
Fortunato, Ana
cgchi3
Casuarina glauca
Chitinase
.
Faculdade de Ciências da Vida
title_short Isolation and characterization of cgchi3, a nodule-specific gene from Casuarina glauca encoding a class III chitinase
title_full Isolation and characterization of cgchi3, a nodule-specific gene from Casuarina glauca encoding a class III chitinase
title_fullStr Isolation and characterization of cgchi3, a nodule-specific gene from Casuarina glauca encoding a class III chitinase
title_full_unstemmed Isolation and characterization of cgchi3, a nodule-specific gene from Casuarina glauca encoding a class III chitinase
title_sort Isolation and characterization of cgchi3, a nodule-specific gene from Casuarina glauca encoding a class III chitinase
author Fortunato, Ana
author_facet Fortunato, Ana
Santos, Patricia
Graça, Inês
Gouveia, Maria Manuela
Martins, Sandra Marina
Ricardo, Cândido Pereira Pinto
Pawlowski, Katharina
Ribeiro, Ana
author_role author
author2 Santos, Patricia
Graça, Inês
Gouveia, Maria Manuela
Martins, Sandra Marina
Ricardo, Cândido Pereira Pinto
Pawlowski, Katharina
Ribeiro, Ana
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DigitUMa
dc.contributor.author.fl_str_mv Fortunato, Ana
Santos, Patricia
Graça, Inês
Gouveia, Maria Manuela
Martins, Sandra Marina
Ricardo, Cândido Pereira Pinto
Pawlowski, Katharina
Ribeiro, Ana
dc.subject.por.fl_str_mv cgchi3
Casuarina glauca
Chitinase
.
Faculdade de Ciências da Vida
topic cgchi3
Casuarina glauca
Chitinase
.
Faculdade de Ciências da Vida
description Chitinases (EC 3.2.1.14) catalyse the hydrolysis of chitin, a homopolymer of b-1,4-linked N-acetyl-D-glucosamine residues. Plant chitinases are involved in a wide variety of processes; in particular, their expression has been found to be enhanced in symbiotic and pathogenic plant–microbe interactions. During this work we have cloned and characterized a gene encoding a class III chitinase from actinorhizal nodules of Casuarina glauca (cgchi3). CGCHI3 was found to be encoded by a single gene that was specifically activated in nodules as compared with uninoculated control roots and leaves. The expression of this gene was further enhanced in nodules after salicylic acid treatment and completely repressed after wounding. In situ hybridisation analysis revealed that cgchi3 is an early nodulin gene, being expressed in the meristem and in the uninfected cortical cells of young nodules. Based on the obtained results we suggest that this gene is involved in nodule development. This is the first report on a class III chitinase coding gene that is specifically activated during actinorhizal symbiosis.
publishDate 2007
dc.date.none.fl_str_mv 2007
2007-01-01T00:00:00Z
2023-07-18T15:19:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.13/5283
url http://hdl.handle.net/10400.13/5283
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fortunato, A., Santos, P., Graça, I., Gouveia, M. M., Martins, S. M., Pinto Ricardo, C. P., ... & Ribeiro, A. (2007). Isolation and characterization of cgchi3, a nodule‐specific gene from Casuarina glauca encoding a class III chitinase. Physiologia Plantarum, 130(3), 418-426.
10.1111/j.1399-3054.2006.00864.x
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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