The Selective Advantage of the lac Operon for Escherichia coli Is Conditional on Diet and Microbiota Composition

Detalhes bibliográficos
Autor(a) principal: Pinto, Catarina
Data de Publicação: 2021
Outros Autores: Melo-Miranda, Rita, Gordo, Isabel, Sousa, Ana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/124272
Resumo: Funding Information: Funding: This work was funded by the Fundação para a Ciência e Tecnologia (PTDC/BIA-EVF/3247/2012 and PTDC/BIAEVL/30212/2017) and iBiMED (UID/BIM/04501/2019, project POCI-01-0145-FEDER-007628). CP was the recipient of a fellowship (138/BI/13) from Fundação para a Ciência e Tecnologia. RM-M was the recipient of a doctoral fellowship (DFA/BD/5130/2020) from Fundação para a Ciência e Tecnologia. AS was funded from National Funds through FCT - Fundação para a Ciência e a Tecnologia, I.P., under the Scientific Employment Stimulus - Institutional Call - reference CEECINST/00026/2018.
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spelling The Selective Advantage of the lac Operon for Escherichia coli Is Conditional on Diet and Microbiota CompositionBacteroides thetaiotaomicronEscherichia colifitness effectgut microbiotalac operonLactobacillus murinuslactosemicrobe–microbe interactionsMicrobiologyMicrobiology (medical)Funding Information: Funding: This work was funded by the Fundação para a Ciência e Tecnologia (PTDC/BIA-EVF/3247/2012 and PTDC/BIAEVL/30212/2017) and iBiMED (UID/BIM/04501/2019, project POCI-01-0145-FEDER-007628). CP was the recipient of a fellowship (138/BI/13) from Fundação para a Ciência e Tecnologia. RM-M was the recipient of a doctoral fellowship (DFA/BD/5130/2020) from Fundação para a Ciência e Tecnologia. AS was funded from National Funds through FCT - Fundação para a Ciência e a Tecnologia, I.P., under the Scientific Employment Stimulus - Institutional Call - reference CEECINST/00026/2018.The lac operon is one of the best known gene regulatory circuits and constitutes a landmark example of how bacteria tune their metabolism to nutritional conditions. It is nearly ubiquitous in Escherichia coli strains justifying the use of its phenotype, the ability to consume lactose, for species identification. Lactose is the primary sugar found in milk, which is abundant in mammals during the first weeks of life. However, lactose is virtually non-existent after the weaning period, with humans being an exception as many consume dairy products throughout their lives. The absence of lactose during adulthood in most mammals and the rarity of lactose in the environment, means that the selective pressure for maintaining the lac operon could be weak for long periods of time. Despite the ability to metabolize lactose being a hallmark of E. coli’s success when colonizing its primary habitat, the mammalian intestine, the selective value of this trait remains unknown in this ecosystem during adulthood. Here we determine the competitive advantage conferred by the lac operon to a commensal strain of E. coli when colonizing the mouse gut. We find that its benefit, which can be as high as 11%, is contingent on the presence of lactose in the diet and on the presence of other microbiota members in the gut, but the operon is never deleterious. These results help explaining the pervasiveness of the lac operon in E. coli, but also its polymorphism, as lac-negative E. coli strains albeit rare can naturally occur in the gut.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNPinto, CatarinaMelo-Miranda, RitaGordo, IsabelSousa, Ana2021-09-09T00:27:42Z2021-07-212021-07-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/124272eng1664-302XPURE: 33428995https://doi.org/10.3389/fmicb.2021.709259info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:05:45Zoai:run.unl.pt:10362/124272Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:45:25.360182Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The Selective Advantage of the lac Operon for Escherichia coli Is Conditional on Diet and Microbiota Composition
title The Selective Advantage of the lac Operon for Escherichia coli Is Conditional on Diet and Microbiota Composition
spellingShingle The Selective Advantage of the lac Operon for Escherichia coli Is Conditional on Diet and Microbiota Composition
Pinto, Catarina
Bacteroides thetaiotaomicron
Escherichia coli
fitness effect
gut microbiota
lac operon
Lactobacillus murinus
lactose
microbe–microbe interactions
Microbiology
Microbiology (medical)
title_short The Selective Advantage of the lac Operon for Escherichia coli Is Conditional on Diet and Microbiota Composition
title_full The Selective Advantage of the lac Operon for Escherichia coli Is Conditional on Diet and Microbiota Composition
title_fullStr The Selective Advantage of the lac Operon for Escherichia coli Is Conditional on Diet and Microbiota Composition
title_full_unstemmed The Selective Advantage of the lac Operon for Escherichia coli Is Conditional on Diet and Microbiota Composition
title_sort The Selective Advantage of the lac Operon for Escherichia coli Is Conditional on Diet and Microbiota Composition
author Pinto, Catarina
author_facet Pinto, Catarina
Melo-Miranda, Rita
Gordo, Isabel
Sousa, Ana
author_role author
author2 Melo-Miranda, Rita
Gordo, Isabel
Sousa, Ana
author2_role author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Pinto, Catarina
Melo-Miranda, Rita
Gordo, Isabel
Sousa, Ana
dc.subject.por.fl_str_mv Bacteroides thetaiotaomicron
Escherichia coli
fitness effect
gut microbiota
lac operon
Lactobacillus murinus
lactose
microbe–microbe interactions
Microbiology
Microbiology (medical)
topic Bacteroides thetaiotaomicron
Escherichia coli
fitness effect
gut microbiota
lac operon
Lactobacillus murinus
lactose
microbe–microbe interactions
Microbiology
Microbiology (medical)
description Funding Information: Funding: This work was funded by the Fundação para a Ciência e Tecnologia (PTDC/BIA-EVF/3247/2012 and PTDC/BIAEVL/30212/2017) and iBiMED (UID/BIM/04501/2019, project POCI-01-0145-FEDER-007628). CP was the recipient of a fellowship (138/BI/13) from Fundação para a Ciência e Tecnologia. RM-M was the recipient of a doctoral fellowship (DFA/BD/5130/2020) from Fundação para a Ciência e Tecnologia. AS was funded from National Funds through FCT - Fundação para a Ciência e a Tecnologia, I.P., under the Scientific Employment Stimulus - Institutional Call - reference CEECINST/00026/2018.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-09T00:27:42Z
2021-07-21
2021-07-21T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/124272
url http://hdl.handle.net/10362/124272
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1664-302X
PURE: 33428995
https://doi.org/10.3389/fmicb.2021.709259
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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