Evaluation of saliva as an alternative to standard collection for detection of SARS-CoV-2
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.48797/sl.2023.20 |
Resumo: | In December 2019, in Wuhan, an outbreak of a new coronavirus (SARS-CoV-2) began to spread rapidly, resulting in a potentially fatal viral respiratory disease. Since August 23, 2020, more than 679 million cases of COVID-19 and nearly 6.8 million deaths have been confirmed in more than 200 countries. This, in turn, had a severe impact on public health and the world economy. The SARS-CoV-2 pandemic also resulted in a shortage of viral transport medium and in the need to find different diagnostic means, such as saliva. To fill this gap in the market, a new viral transport medium was created and tested with samples collected from the nasopharynx and/or oropharynx using swabs and saliva samples. The specificity, sensitivity, and threshold cycles (Cts) of Real-Time PCR (RT-PCR) testing of the samples were compared, revealing concordant results with approximate sensitivity and specificity. Our study highlighted the need to optimize saliva sample collection and its potential use as a substitute for standard collection. |
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Evaluation of saliva as an alternative to standard collection for detection of SARS-CoV-2SARS-CoV-2salivaviral transport mediumIn December 2019, in Wuhan, an outbreak of a new coronavirus (SARS-CoV-2) began to spread rapidly, resulting in a potentially fatal viral respiratory disease. Since August 23, 2020, more than 679 million cases of COVID-19 and nearly 6.8 million deaths have been confirmed in more than 200 countries. This, in turn, had a severe impact on public health and the world economy. The SARS-CoV-2 pandemic also resulted in a shortage of viral transport medium and in the need to find different diagnostic means, such as saliva. To fill this gap in the market, a new viral transport medium was created and tested with samples collected from the nasopharynx and/or oropharynx using swabs and saliva samples. The specificity, sensitivity, and threshold cycles (Cts) of Real-Time PCR (RT-PCR) testing of the samples were compared, revealing concordant results with approximate sensitivity and specificity. Our study highlighted the need to optimize saliva sample collection and its potential use as a substitute for standard collection.IUCS-CESPU Publishing2023-05-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.48797/sl.2023.20https://doi.org/10.48797/sl.2023.20Scientific Letters; Vol. 1 No. 1 (2023)2795-5117reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttps://publicacoes.cespu.pt/index.php/sl/article/view/20https://publicacoes.cespu.pt/index.php/sl/article/view/20/130Copyright (c) 2023 Fernando Ferreira, Daniela Alves, Telma Oliveira, Silvia Coelho, Inês Oliveira, Paula de Melo Alvesinfo:eu-repo/semantics/openAccessFerreira, FernandoAlves, DanielaOliveira, TelmaCoelho, SilviaOliveira, Inêsde Melo Alves, Paula2023-06-10T08:45:13Zoai:publicacoes.cespu.pt:article/20Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:00:08.957911Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evaluation of saliva as an alternative to standard collection for detection of SARS-CoV-2 |
title |
Evaluation of saliva as an alternative to standard collection for detection of SARS-CoV-2 |
spellingShingle |
Evaluation of saliva as an alternative to standard collection for detection of SARS-CoV-2 Ferreira, Fernando SARS-CoV-2 saliva viral transport medium |
title_short |
Evaluation of saliva as an alternative to standard collection for detection of SARS-CoV-2 |
title_full |
Evaluation of saliva as an alternative to standard collection for detection of SARS-CoV-2 |
title_fullStr |
Evaluation of saliva as an alternative to standard collection for detection of SARS-CoV-2 |
title_full_unstemmed |
Evaluation of saliva as an alternative to standard collection for detection of SARS-CoV-2 |
title_sort |
Evaluation of saliva as an alternative to standard collection for detection of SARS-CoV-2 |
author |
Ferreira, Fernando |
author_facet |
Ferreira, Fernando Alves, Daniela Oliveira, Telma Coelho, Silvia Oliveira, Inês de Melo Alves, Paula |
author_role |
author |
author2 |
Alves, Daniela Oliveira, Telma Coelho, Silvia Oliveira, Inês de Melo Alves, Paula |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira, Fernando Alves, Daniela Oliveira, Telma Coelho, Silvia Oliveira, Inês de Melo Alves, Paula |
dc.subject.por.fl_str_mv |
SARS-CoV-2 saliva viral transport medium |
topic |
SARS-CoV-2 saliva viral transport medium |
description |
In December 2019, in Wuhan, an outbreak of a new coronavirus (SARS-CoV-2) began to spread rapidly, resulting in a potentially fatal viral respiratory disease. Since August 23, 2020, more than 679 million cases of COVID-19 and nearly 6.8 million deaths have been confirmed in more than 200 countries. This, in turn, had a severe impact on public health and the world economy. The SARS-CoV-2 pandemic also resulted in a shortage of viral transport medium and in the need to find different diagnostic means, such as saliva. To fill this gap in the market, a new viral transport medium was created and tested with samples collected from the nasopharynx and/or oropharynx using swabs and saliva samples. The specificity, sensitivity, and threshold cycles (Cts) of Real-Time PCR (RT-PCR) testing of the samples were compared, revealing concordant results with approximate sensitivity and specificity. Our study highlighted the need to optimize saliva sample collection and its potential use as a substitute for standard collection. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.48797/sl.2023.20 https://doi.org/10.48797/sl.2023.20 |
url |
https://doi.org/10.48797/sl.2023.20 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://publicacoes.cespu.pt/index.php/sl/article/view/20 https://publicacoes.cespu.pt/index.php/sl/article/view/20/130 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
IUCS-CESPU Publishing |
publisher.none.fl_str_mv |
IUCS-CESPU Publishing |
dc.source.none.fl_str_mv |
Scientific Letters; Vol. 1 No. 1 (2023) 2795-5117 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131670906929152 |