Using GFP as a biomarker to visualize the process of bacterial infection in black carp (Mylopharyngodon piceus)

Detalhes bibliográficos
Autor(a) principal: Zhang, Xueshu
Data de Publicação: 2020
Outros Autores: Xu, Xiaoyan, Shen, Yubang, Li, Lisen, Wang, Rongquan, Li, Jiale
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/14975
Resumo: As one of the most critical pathogens, Aeromonas hydrophila (AH) can cause motile aeromonad septicemia (MAS) in freshwater fish. In recent decades, a myriad of studies had been done for bacterial infect fish. However, the mechanism of bacterial infects fish especially freshwater fish was scanty. This study was conducted for investigating the invasion pathway of A. hydrophila in vivo of black carp. We have performed A. hydrophila 4332 transformed with a plasmid encoding the green fluorescent protein (pGFPuv) (AH4332GFPuv) in black carp. The AH4332GFPuv had similar growth properties and virulence as the wild-type strains under the simulated natural condition. In this study, black carp were divided into five groups: IM (challenged via immersion), IBD (increased stocking density), SAW (skin artificially wounded by scalpel), MR (mucus removed from the body surface), and C0 (control group). The number of AH4332GFPuv in gill, liver, spleen, intestine, mid kidney, head kidney, muscle, eye, brain, heart, and blood were examined after 72 h post-infection from all groups. Significantly high bacterial numbers were observed in the gills and intestine. The number of bacteria was significantly higher in IBD group than IM group. In conclusion, the gill, intestines, and injured skin are likely to be the primary infection routes.
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spelling Using GFP as a biomarker to visualize the process of bacterial infection in black carp (Mylopharyngodon piceus)Green fluorescent proteinInfection dynamicsMylopharyngodon piceusAeromonas hydrophilaAs one of the most critical pathogens, Aeromonas hydrophila (AH) can cause motile aeromonad septicemia (MAS) in freshwater fish. In recent decades, a myriad of studies had been done for bacterial infect fish. However, the mechanism of bacterial infects fish especially freshwater fish was scanty. This study was conducted for investigating the invasion pathway of A. hydrophila in vivo of black carp. We have performed A. hydrophila 4332 transformed with a plasmid encoding the green fluorescent protein (pGFPuv) (AH4332GFPuv) in black carp. The AH4332GFPuv had similar growth properties and virulence as the wild-type strains under the simulated natural condition. In this study, black carp were divided into five groups: IM (challenged via immersion), IBD (increased stocking density), SAW (skin artificially wounded by scalpel), MR (mucus removed from the body surface), and C0 (control group). The number of AH4332GFPuv in gill, liver, spleen, intestine, mid kidney, head kidney, muscle, eye, brain, heart, and blood were examined after 72 h post-infection from all groups. Significantly high bacterial numbers were observed in the gills and intestine. The number of bacteria was significantly higher in IBD group than IM group. In conclusion, the gill, intestines, and injured skin are likely to be the primary infection routes.ElsevierSapientiaZhang, XueshuXu, XiaoyanShen, YubangLi, LisenWang, RongquanLi, Jiale2021-01-19T14:11:19Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/14975eng10.1016/j.aqrep.2020.100530info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:27:20Zoai:sapientia.ualg.pt:10400.1/14975Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:05:54.502903Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Using GFP as a biomarker to visualize the process of bacterial infection in black carp (Mylopharyngodon piceus)
title Using GFP as a biomarker to visualize the process of bacterial infection in black carp (Mylopharyngodon piceus)
spellingShingle Using GFP as a biomarker to visualize the process of bacterial infection in black carp (Mylopharyngodon piceus)
Zhang, Xueshu
Green fluorescent protein
Infection dynamics
Mylopharyngodon piceus
Aeromonas hydrophila
title_short Using GFP as a biomarker to visualize the process of bacterial infection in black carp (Mylopharyngodon piceus)
title_full Using GFP as a biomarker to visualize the process of bacterial infection in black carp (Mylopharyngodon piceus)
title_fullStr Using GFP as a biomarker to visualize the process of bacterial infection in black carp (Mylopharyngodon piceus)
title_full_unstemmed Using GFP as a biomarker to visualize the process of bacterial infection in black carp (Mylopharyngodon piceus)
title_sort Using GFP as a biomarker to visualize the process of bacterial infection in black carp (Mylopharyngodon piceus)
author Zhang, Xueshu
author_facet Zhang, Xueshu
Xu, Xiaoyan
Shen, Yubang
Li, Lisen
Wang, Rongquan
Li, Jiale
author_role author
author2 Xu, Xiaoyan
Shen, Yubang
Li, Lisen
Wang, Rongquan
Li, Jiale
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Zhang, Xueshu
Xu, Xiaoyan
Shen, Yubang
Li, Lisen
Wang, Rongquan
Li, Jiale
dc.subject.por.fl_str_mv Green fluorescent protein
Infection dynamics
Mylopharyngodon piceus
Aeromonas hydrophila
topic Green fluorescent protein
Infection dynamics
Mylopharyngodon piceus
Aeromonas hydrophila
description As one of the most critical pathogens, Aeromonas hydrophila (AH) can cause motile aeromonad septicemia (MAS) in freshwater fish. In recent decades, a myriad of studies had been done for bacterial infect fish. However, the mechanism of bacterial infects fish especially freshwater fish was scanty. This study was conducted for investigating the invasion pathway of A. hydrophila in vivo of black carp. We have performed A. hydrophila 4332 transformed with a plasmid encoding the green fluorescent protein (pGFPuv) (AH4332GFPuv) in black carp. The AH4332GFPuv had similar growth properties and virulence as the wild-type strains under the simulated natural condition. In this study, black carp were divided into five groups: IM (challenged via immersion), IBD (increased stocking density), SAW (skin artificially wounded by scalpel), MR (mucus removed from the body surface), and C0 (control group). The number of AH4332GFPuv in gill, liver, spleen, intestine, mid kidney, head kidney, muscle, eye, brain, heart, and blood were examined after 72 h post-infection from all groups. Significantly high bacterial numbers were observed in the gills and intestine. The number of bacteria was significantly higher in IBD group than IM group. In conclusion, the gill, intestines, and injured skin are likely to be the primary infection routes.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2021-01-19T14:11:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/14975
url http://hdl.handle.net/10400.1/14975
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.aqrep.2020.100530
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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