Biochemical, biophysical and haemorheological effects of dimethylsulphoxide on human erythrocyte calcium loading

Detalhes bibliográficos
Autor(a) principal: Santos, Nuno C.
Data de Publicação: 2002
Outros Autores: Figueira-Coelho, J., Saldanha, Carlota, Martins-Silva, João
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/17630
Resumo: © 2002, Published by Elsevier Science Ltd.
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spelling Biochemical, biophysical and haemorheological effects of dimethylsulphoxide on human erythrocyte calcium loading© 2002, Published by Elsevier Science Ltd.The studies using dimethylsulphoxide (DMSO) and/or the 4-bromo-calcium ionophore A23187 (Br-A23187)often neglect the precise knowledge of some of their biochemical, biophysical and haemorheological effects. The aim of the present study was to evaluate these effects on erythrocytes after whole blood incubations with DMSO or Br-A23187 dissolved in DMSO. There were no significant differences between the different aliquots in the values of P50, pH,erythrocyte deformability, erythrocyte membrane fluidity, haemoglobin and intracellular Ca2+ concentrations ([Ca2+]i). Aliquots with DMSO (independently of the presence of Br-A23187 or added Ca2+) had lower erythrocyte aggregation indexes and higher plasma concentrations of K+, Na+ and Ca2+ than the aliquots without DMSO independently of the presence of added Ca2+). Aliquots with added calcium (without the presence of Br-A23187 in DMSO) had a significantly higher erythrocyte acetylcholinesterase activity. Our data shows that calcium loading, the usual objective of Br-A23187 incubations, cannot be fulfilled with the studied experimental conditions. The coherence between our results and those obtained by other authors with different biological systems and different modulators of the rise on [Ca2+]i suggests a non-specific effect of DMSO, disabling the action of the modulator. It can be reasoned that the decreased erythrocyte aggregation (without significant changes on the deformability or membrane fluidity) can result either from the decrease of the hydrogen bonding contribution to erythrocyte aggregation or the increased ionic strength influence on the erythrocyte membrane surface.This work was supported by the Fundação para a Ciência e Tecnologia of the Portuguese Ministry of Science and Technology, and by the Calouste Gulbenkian Foundation (Portugal)ElsevierRepositório da Universidade de LisboaSantos, Nuno C.Figueira-Coelho, J.Saldanha, CarlotaMartins-Silva, João2015-03-05T14:49:06Z20022002-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/17630engCell Calcium (2002) 31(4), 183–1880143-4160http://dx.doi.org/10.1054/ceca.2002.0271metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:22:11Zoai:repositorio.ul.pt:10451/17630Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:22:11Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biochemical, biophysical and haemorheological effects of dimethylsulphoxide on human erythrocyte calcium loading
title Biochemical, biophysical and haemorheological effects of dimethylsulphoxide on human erythrocyte calcium loading
spellingShingle Biochemical, biophysical and haemorheological effects of dimethylsulphoxide on human erythrocyte calcium loading
Santos, Nuno C.
title_short Biochemical, biophysical and haemorheological effects of dimethylsulphoxide on human erythrocyte calcium loading
title_full Biochemical, biophysical and haemorheological effects of dimethylsulphoxide on human erythrocyte calcium loading
title_fullStr Biochemical, biophysical and haemorheological effects of dimethylsulphoxide on human erythrocyte calcium loading
title_full_unstemmed Biochemical, biophysical and haemorheological effects of dimethylsulphoxide on human erythrocyte calcium loading
title_sort Biochemical, biophysical and haemorheological effects of dimethylsulphoxide on human erythrocyte calcium loading
author Santos, Nuno C.
author_facet Santos, Nuno C.
Figueira-Coelho, J.
Saldanha, Carlota
Martins-Silva, João
author_role author
author2 Figueira-Coelho, J.
Saldanha, Carlota
Martins-Silva, João
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Santos, Nuno C.
Figueira-Coelho, J.
Saldanha, Carlota
Martins-Silva, João
description © 2002, Published by Elsevier Science Ltd.
publishDate 2002
dc.date.none.fl_str_mv 2002
2002-01-01T00:00:00Z
2015-03-05T14:49:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/17630
url http://hdl.handle.net/10451/17630
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cell Calcium (2002) 31(4), 183–188
0143-4160
http://dx.doi.org/10.1054/ceca.2002.0271
dc.rights.driver.fl_str_mv metadata only access
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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