Use of aspartic acid racemization in fingerprints as a molecular clock

Detalhes bibliográficos
Autor(a) principal: Adelantado Sánchez, Carlos
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.26/6716
Resumo: Dissertação de Erasmus Mundus para obtenção do grau de mestre em Técnicas Laboratoriais Forenses
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spelling Use of aspartic acid racemization in fingerprints as a molecular clockAspartic acidIsomersRacemizationFingerprintsDissertação de Erasmus Mundus para obtenção do grau de mestre em Técnicas Laboratoriais ForensesAspartic acid is the chemical, amongst amino acids, whose racemization process is considered to be the fastest and the most reliable way to conduct an ageing study in some living-being tissues. Racemization is a natural-occurring event in which L-stereoisomer of aspartic acid is converted into D-stereoisomer in an equilibrium dependent on factors namely pH, environmental conditions and, most critically, on temperature.The main objective of the current study is to assess the influence of temperature on kinetics of aspartic acid racemization and to implement this methodology in fingermarks. The hypothesis is that different starting points of deposition of a biological specimen will give rise to significant differences in L-/D- racemization after the sample has been heated, allowing to determine time since deposition of a fingerprint. In this way, it would be possible to find the time since build a molecular clock for fingerprints, consisting of time since deposition of fingermarks based on racemization degree of aspartic acid.Firstly, a calibration curve was built within the concentrations range expected to be quantified in actual samples both with L-aspartic acid (L-Asp) and D-aspartic acid (D-Asp) separately, Gas Chromatography - Flame Ionization Detector (GC-FID) being the chosen technique and detection mode to set up the calibration experiments. Secondly, L-aspartic acid standards were treated at different temperatures during different time periods and were analyzed by GC-FID with a chiral column, aimed for detecting D- isomer, providing with information about racemization rate of aspartic acid.Eventually a downward trend was observed for L-Asp but not for D-Asp and a pseudo-rate constant could be calculated for the former, demonstrating that temperature degrades this chemical. Fingerprints study was not successful to determine aspartic acid racemization but a simple handling of them and an adequate, sensitive response of the chromatographic system could quantify racemization degree.Instituto Superior de Ciências da Saúde Egas MonizQuintas, AlexandreSoeiro, RitaRepositório ComumAdelantado Sánchez, Carlos2014-09-18T11:45:09Z2014-07-01T00:00:00Z2014-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.26/6716201541475enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-10-06T14:51:29Zoai:comum.rcaap.pt:10400.26/6716Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:06:05.592505Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Use of aspartic acid racemization in fingerprints as a molecular clock
title Use of aspartic acid racemization in fingerprints as a molecular clock
spellingShingle Use of aspartic acid racemization in fingerprints as a molecular clock
Adelantado Sánchez, Carlos
Aspartic acid
Isomers
Racemization
Fingerprints
title_short Use of aspartic acid racemization in fingerprints as a molecular clock
title_full Use of aspartic acid racemization in fingerprints as a molecular clock
title_fullStr Use of aspartic acid racemization in fingerprints as a molecular clock
title_full_unstemmed Use of aspartic acid racemization in fingerprints as a molecular clock
title_sort Use of aspartic acid racemization in fingerprints as a molecular clock
author Adelantado Sánchez, Carlos
author_facet Adelantado Sánchez, Carlos
author_role author
dc.contributor.none.fl_str_mv Quintas, Alexandre
Soeiro, Rita
Repositório Comum
dc.contributor.author.fl_str_mv Adelantado Sánchez, Carlos
dc.subject.por.fl_str_mv Aspartic acid
Isomers
Racemization
Fingerprints
topic Aspartic acid
Isomers
Racemization
Fingerprints
description Dissertação de Erasmus Mundus para obtenção do grau de mestre em Técnicas Laboratoriais Forenses
publishDate 2014
dc.date.none.fl_str_mv 2014-09-18T11:45:09Z
2014-07-01T00:00:00Z
2014-07-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.26/6716
201541475
url http://hdl.handle.net/10400.26/6716
identifier_str_mv 201541475
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Superior de Ciências da Saúde Egas Moniz
publisher.none.fl_str_mv Instituto Superior de Ciências da Saúde Egas Moniz
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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