Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties

Detalhes bibliográficos
Autor(a) principal: Sampaio, Liliana M. P.
Data de Publicação: 2016
Outros Autores: Padrão, Jorge, Faria, Jorge, Silva, João P., Silva, Carla J., Dourado, Fernando, Zille, Andrea
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/41296
Resumo: This work studied the physical immobilization of a commercial laccase on bacterial nanocellulose (BNC) aiming to identify the laccase antibacterial properties suitable for wound dressings. Physico-chemical analysis demonstrates that the BNC structure is manly formed by pure crystalline I cellulose. The pH optimum and activation energy of free laccase depends on the substrate employed corresponding to pH 6, 7, 3 and 57, 22, 48 kJ mol1 for 2,6-dimethylphenol (DMP), catechol and 2,2 -azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), respectively. The Michaelis-Menten constant (Km) value for the immobilized laccase (0.77 mM) was found to be almost double of that of the free enzyme (0.42 mM). However, the specific activities of immobilized and free laccase are similar suggesting that the cage-like structure of BNC allows entrapped laccase to maintain some flexibility and favour substrate accessibility. The results clearly show the antimicrobial effect of laccase in Gram-positive (92%) and Gram-negative (26%) bacteria and cytotoxicity acceptable for wound dressing applications.
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spelling Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability propertiesBacterial CelluloseLaccaseAntimicrobialWound DressingImmobilizationCytotoxicityScience & TechnologyThis work studied the physical immobilization of a commercial laccase on bacterial nanocellulose (BNC) aiming to identify the laccase antibacterial properties suitable for wound dressings. Physico-chemical analysis demonstrates that the BNC structure is manly formed by pure crystalline I cellulose. The pH optimum and activation energy of free laccase depends on the substrate employed corresponding to pH 6, 7, 3 and 57, 22, 48 kJ mol1 for 2,6-dimethylphenol (DMP), catechol and 2,2 -azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), respectively. The Michaelis-Menten constant (Km) value for the immobilized laccase (0.77 mM) was found to be almost double of that of the free enzyme (0.42 mM). However, the specific activities of immobilized and free laccase are similar suggesting that the cage-like structure of BNC allows entrapped laccase to maintain some flexibility and favour substrate accessibility. The results clearly show the antimicrobial effect of laccase in Gram-positive (92%) and Gram-negative (26%) bacteria and cytotoxicity acceptable for wound dressing applications.Andrea Zille (C2011-UMINHO-2C2T-01) acknowledges FCT funding from Programa Compromisso para a Ciência 2008, Portugal, FEDER funding on the Programa Operacional Factores de Competitividade - COMPETE and by national funds through FCT – Foundation for Science and Technology within the scope of the project POCI-01-0145-FEDER-007136 and UID/CTM/00264. Jorge Padrão and João P. Silva acknowledge Portuguese Foundation for Science and Technology (FCT) grants SFRH/BD/64901/2009 and SFRH/BPD/64958/2009 respectively. The authors Jorge Padrão, JoãoP. Silva and Fernando Dourado would like to thank the FCT StrategicProject PEst-OE/EQB/LA0023/2013, also to the Project “BioHealth −Biotechnology and Bioengineering approaches to improve healthquality”, Ref. NORTE-07-0124-FEDER-000027, co-funded by thePrograma Operacional Regional do Norte (ON.2-O Novo Norte),QREN, FEDER, and finnally to RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462).Elsevier 1Universidade do MinhoSampaio, Liliana M. P.Padrão, JorgeFaria, JorgeSilva, João P.Silva, Carla J.Dourado, FernandoZille, Andrea2016-07-102016-07-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/41296engSampaio, Liliana M. P.; Padrão, Jorge; Faria, Jorge; Silva, João P.; Silva, Carla J.; Dourado, Fernando; Zille, Andrea, Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties. Carbohydrate Polymers, 145, 1-12, 20160144-86170144-861710.1016/j.carbpol.2016.03.00927106145http://www.journals.elsevier.com/carbohydrate-polymers/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-25T01:55:36Zoai:repositorium.sdum.uminho.pt:1822/41296Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-25T01:55:36Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties
title Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties
spellingShingle Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties
Sampaio, Liliana M. P.
Bacterial Cellulose
Laccase
Antimicrobial
Wound Dressing
Immobilization
Cytotoxicity
Science & Technology
title_short Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties
title_full Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties
title_fullStr Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties
title_full_unstemmed Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties
title_sort Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties
author Sampaio, Liliana M. P.
author_facet Sampaio, Liliana M. P.
Padrão, Jorge
Faria, Jorge
Silva, João P.
Silva, Carla J.
Dourado, Fernando
Zille, Andrea
author_role author
author2 Padrão, Jorge
Faria, Jorge
Silva, João P.
Silva, Carla J.
Dourado, Fernando
Zille, Andrea
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Sampaio, Liliana M. P.
Padrão, Jorge
Faria, Jorge
Silva, João P.
Silva, Carla J.
Dourado, Fernando
Zille, Andrea
dc.subject.por.fl_str_mv Bacterial Cellulose
Laccase
Antimicrobial
Wound Dressing
Immobilization
Cytotoxicity
Science & Technology
topic Bacterial Cellulose
Laccase
Antimicrobial
Wound Dressing
Immobilization
Cytotoxicity
Science & Technology
description This work studied the physical immobilization of a commercial laccase on bacterial nanocellulose (BNC) aiming to identify the laccase antibacterial properties suitable for wound dressings. Physico-chemical analysis demonstrates that the BNC structure is manly formed by pure crystalline I cellulose. The pH optimum and activation energy of free laccase depends on the substrate employed corresponding to pH 6, 7, 3 and 57, 22, 48 kJ mol1 for 2,6-dimethylphenol (DMP), catechol and 2,2 -azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), respectively. The Michaelis-Menten constant (Km) value for the immobilized laccase (0.77 mM) was found to be almost double of that of the free enzyme (0.42 mM). However, the specific activities of immobilized and free laccase are similar suggesting that the cage-like structure of BNC allows entrapped laccase to maintain some flexibility and favour substrate accessibility. The results clearly show the antimicrobial effect of laccase in Gram-positive (92%) and Gram-negative (26%) bacteria and cytotoxicity acceptable for wound dressing applications.
publishDate 2016
dc.date.none.fl_str_mv 2016-07-10
2016-07-10T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/41296
url https://hdl.handle.net/1822/41296
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Sampaio, Liliana M. P.; Padrão, Jorge; Faria, Jorge; Silva, João P.; Silva, Carla J.; Dourado, Fernando; Zille, Andrea, Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties. Carbohydrate Polymers, 145, 1-12, 2016
0144-8617
0144-8617
10.1016/j.carbpol.2016.03.009
27106145
http://www.journals.elsevier.com/carbohydrate-polymers/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier 1
publisher.none.fl_str_mv Elsevier 1
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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