Dual-mode gold nanoparticle-based method for early detection of Acanthamoeba
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/81037 |
Resumo: | Acanthamoeba keratitis is an aggressive and rapidly progressing ocular pathology whose main risk factor is the use of contact lenses. An early and differential diagnosis is considered the main factor to prevent the progression and improve the prognosis of the pathology. However, current diagnosis techniques require time, complex and costly materials making an early diagnosis challenging. Thus, there is a need for fast, accessible, and accurate methods for Acanthamoeba detection by practitioners for timely and suitable treatment and even for contact lens user as preventive diagnosis. Here, we developed a dual-mode colorimetric-based method for fast, visual, and accurate detection of Acanthamoeba using gold nanoparticles (AuNPs). For this strategy, AuNPs were functionalized with thiolated probes and the presence of target Acanthamoeba genomic sequences, produce a colorimetric change from red to purple. This approach allows the detection of 0.02 and 0.009 μM of the unamplified Acanthamoeba genome by the naked eye in less than 20 min and by color analysis using a smartphone. Additionally, real samples were successfully analyzed showing the potential of the technology considering the lack of point-of-care tools that are mostly needed. |
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Dual-mode gold nanoparticle-based method for early detection of AcanthamoebaAcanthamoebaAcanthamoeba keratitisgold nanoparticlescolorimetric assaycolor coordinatesoptical detectionScience & TechnologyAcanthamoeba keratitis is an aggressive and rapidly progressing ocular pathology whose main risk factor is the use of contact lenses. An early and differential diagnosis is considered the main factor to prevent the progression and improve the prognosis of the pathology. However, current diagnosis techniques require time, complex and costly materials making an early diagnosis challenging. Thus, there is a need for fast, accessible, and accurate methods for Acanthamoeba detection by practitioners for timely and suitable treatment and even for contact lens user as preventive diagnosis. Here, we developed a dual-mode colorimetric-based method for fast, visual, and accurate detection of Acanthamoeba using gold nanoparticles (AuNPs). For this strategy, AuNPs were functionalized with thiolated probes and the presence of target Acanthamoeba genomic sequences, produce a colorimetric change from red to purple. This approach allows the detection of 0.02 and 0.009 μM of the unamplified Acanthamoeba genome by the naked eye in less than 20 min and by color analysis using a smartphone. Additionally, real samples were successfully analyzed showing the potential of the technology considering the lack of point-of-care tools that are mostly needed.This work was supported by an industrial project (ND2017/BMD7676) granted by the community of Madrid.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoPastrana, CristinaGuerreiro, JoanaElumalai, MonishaCarpena-Torres, CarlosCrooke, AlmudenaCarracedo, GonzaloPrado, MartaHuete-Toral, Fernando20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/81037engPastrana, C.; Guerreiro, J.R.L.; Elumalai, M.; Carpena-Torres, C.; Crooke, A.; Carracedo, G.; Prado, M.; Huete-Toral, F. Dual-Mode Gold Nanoparticle-Based Method for Early Detection of Acanthamoeba. Int. J. Mol. Sci. 2022, 23, 14877. https://doi.org/10.3390/ijms2323148771424-67831661-65961422-006710.3390/ijms23231487736499204https://www.mdpi.com/1422-0067/23/23/14877info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:07Zoai:repositorium.sdum.uminho.pt:1822/81037Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:18.994821Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Dual-mode gold nanoparticle-based method for early detection of Acanthamoeba |
title |
Dual-mode gold nanoparticle-based method for early detection of Acanthamoeba |
spellingShingle |
Dual-mode gold nanoparticle-based method for early detection of Acanthamoeba Pastrana, Cristina Acanthamoeba Acanthamoeba keratitis gold nanoparticles colorimetric assay color coordinates optical detection Science & Technology |
title_short |
Dual-mode gold nanoparticle-based method for early detection of Acanthamoeba |
title_full |
Dual-mode gold nanoparticle-based method for early detection of Acanthamoeba |
title_fullStr |
Dual-mode gold nanoparticle-based method for early detection of Acanthamoeba |
title_full_unstemmed |
Dual-mode gold nanoparticle-based method for early detection of Acanthamoeba |
title_sort |
Dual-mode gold nanoparticle-based method for early detection of Acanthamoeba |
author |
Pastrana, Cristina |
author_facet |
Pastrana, Cristina Guerreiro, Joana Elumalai, Monisha Carpena-Torres, Carlos Crooke, Almudena Carracedo, Gonzalo Prado, Marta Huete-Toral, Fernando |
author_role |
author |
author2 |
Guerreiro, Joana Elumalai, Monisha Carpena-Torres, Carlos Crooke, Almudena Carracedo, Gonzalo Prado, Marta Huete-Toral, Fernando |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pastrana, Cristina Guerreiro, Joana Elumalai, Monisha Carpena-Torres, Carlos Crooke, Almudena Carracedo, Gonzalo Prado, Marta Huete-Toral, Fernando |
dc.subject.por.fl_str_mv |
Acanthamoeba Acanthamoeba keratitis gold nanoparticles colorimetric assay color coordinates optical detection Science & Technology |
topic |
Acanthamoeba Acanthamoeba keratitis gold nanoparticles colorimetric assay color coordinates optical detection Science & Technology |
description |
Acanthamoeba keratitis is an aggressive and rapidly progressing ocular pathology whose main risk factor is the use of contact lenses. An early and differential diagnosis is considered the main factor to prevent the progression and improve the prognosis of the pathology. However, current diagnosis techniques require time, complex and costly materials making an early diagnosis challenging. Thus, there is a need for fast, accessible, and accurate methods for Acanthamoeba detection by practitioners for timely and suitable treatment and even for contact lens user as preventive diagnosis. Here, we developed a dual-mode colorimetric-based method for fast, visual, and accurate detection of Acanthamoeba using gold nanoparticles (AuNPs). For this strategy, AuNPs were functionalized with thiolated probes and the presence of target Acanthamoeba genomic sequences, produce a colorimetric change from red to purple. This approach allows the detection of 0.02 and 0.009 μM of the unamplified Acanthamoeba genome by the naked eye in less than 20 min and by color analysis using a smartphone. Additionally, real samples were successfully analyzed showing the potential of the technology considering the lack of point-of-care tools that are mostly needed. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2022-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/81037 |
url |
https://hdl.handle.net/1822/81037 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pastrana, C.; Guerreiro, J.R.L.; Elumalai, M.; Carpena-Torres, C.; Crooke, A.; Carracedo, G.; Prado, M.; Huete-Toral, F. Dual-Mode Gold Nanoparticle-Based Method for Early Detection of Acanthamoeba. Int. J. Mol. Sci. 2022, 23, 14877. https://doi.org/10.3390/ijms232314877 1424-6783 1661-6596 1422-0067 10.3390/ijms232314877 36499204 https://www.mdpi.com/1422-0067/23/23/14877 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132585532588032 |