Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortium

Detalhes bibliográficos
Autor(a) principal: Pacheco, C.C.
Data de Publicação: 2003
Outros Autores: Alves, C.C., Barreiros, L., Castro, Paula M. L., Teixeira, Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/6835
Resumo: Epifluorescence microscope methods, namely BacLight, direct epifluorescence filter technique and Rhodamine 123, consistently underestimated plate bacterial counts in a 4-chlorophenol degrading consortium. Cells capable of passing through 0.2 mgrm filters, referred as `ultramicrocells', were found. Although cell counts were higher when traditional methods were used, BacLight and direct epifluorescence filter technique were convenient techniques for the systematic monitoring of bacteria involved in biodegradation processes, as results were consistent and available within a short time.
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spelling Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortiumDegrading consortiaFluorescence dyesStarvationUltramicrocellsViable but non-culturable bacteriaEpifluorescence microscope methods, namely BacLight, direct epifluorescence filter technique and Rhodamine 123, consistently underestimated plate bacterial counts in a 4-chlorophenol degrading consortium. Cells capable of passing through 0.2 mgrm filters, referred as `ultramicrocells', were found. Although cell counts were higher when traditional methods were used, BacLight and direct epifluorescence filter technique were convenient techniques for the systematic monitoring of bacteria involved in biodegradation processes, as results were consistent and available within a short time.Springer VerlagVeritati - Repositório Institucional da Universidade Católica PortuguesaPacheco, C.C.Alves, C.C.Barreiros, L.Castro, Paula M. L.Teixeira, Paula2011-10-22T16:06:55Z20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/6835engPACHECO, C.C... [et al.] - Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortium. Biotechnology Letters. ISSN 1573-6776. Vol. 25, n.º 24 (2003) p. 2089-209210.1023/B:BILE.0000007070.12514.64info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:10:32ZPortal AgregadorONG
dc.title.none.fl_str_mv Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortium
title Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortium
spellingShingle Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortium
Pacheco, C.C.
Degrading consortia
Fluorescence dyes
Starvation
Ultramicrocells
Viable but non-culturable bacteria
title_short Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortium
title_full Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortium
title_fullStr Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortium
title_full_unstemmed Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortium
title_sort Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortium
author Pacheco, C.C.
author_facet Pacheco, C.C.
Alves, C.C.
Barreiros, L.
Castro, Paula M. L.
Teixeira, Paula
author_role author
author2 Alves, C.C.
Barreiros, L.
Castro, Paula M. L.
Teixeira, Paula
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Pacheco, C.C.
Alves, C.C.
Barreiros, L.
Castro, Paula M. L.
Teixeira, Paula
dc.subject.por.fl_str_mv Degrading consortia
Fluorescence dyes
Starvation
Ultramicrocells
Viable but non-culturable bacteria
topic Degrading consortia
Fluorescence dyes
Starvation
Ultramicrocells
Viable but non-culturable bacteria
description Epifluorescence microscope methods, namely BacLight, direct epifluorescence filter technique and Rhodamine 123, consistently underestimated plate bacterial counts in a 4-chlorophenol degrading consortium. Cells capable of passing through 0.2 mgrm filters, referred as `ultramicrocells', were found. Although cell counts were higher when traditional methods were used, BacLight and direct epifluorescence filter technique were convenient techniques for the systematic monitoring of bacteria involved in biodegradation processes, as results were consistent and available within a short time.
publishDate 2003
dc.date.none.fl_str_mv 2003
2003-01-01T00:00:00Z
2011-10-22T16:06:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/6835
url http://hdl.handle.net/10400.14/6835
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PACHECO, C.C... [et al.] - Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortium. Biotechnology Letters. ISSN 1573-6776. Vol. 25, n.º 24 (2003) p. 2089-2092
10.1023/B:BILE.0000007070.12514.64
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv
repository.mail.fl_str_mv
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