A bacteriophage detection tool for viability assessment of Salmonella cells

Detalhes bibliográficos
Autor(a) principal: Fernandes, Elisabete Ramos
Data de Publicação: 2014
Outros Autores: Martins, V. C., Nóbrega, Cláudia, Carvalho, Carla A. O. C. M., Cardoso, F. A., Cardoso, S., Dias, J., Deng, D., Kluskens, Leon, Freitas, P. P., Azeredo, Joana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/27313
Resumo: Available online 7 September 2013
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spelling A bacteriophage detection tool for viability assessment of Salmonella cellsBacteriophageSalmonellaCell viabilityViable but non-culturable bacteriaMagnetoresistive biochipMagnetic nanoparticlesScience & TechnologyAvailable online 7 September 2013Salmonellosis, one of the most common food and water-borne diseases, has a major global health and economic impact. Salmonella cells present high infection rates, persistence over inauspicious conditions and the potential to preserve virulence in dormant states when cells are viable but non-culturable (VBNC). These facts are challenging for current detection methods. Culture methods lack the capacity to detect VBNC cells, while biomolecular methods (e.g. DNA- or protein-based) hardly distinguish between dead innocuous cells and their viable lethal counterparts. This work presents and validates a novel bacteriophage (phage)-based microbial detection tool to detect and assess Salmonella viability. Salmonella Enteritidis cells in a VBNC physiological state were evaluated by cell culture, flow-cytometry and epifluorescence microscopy, and further assayed with a biosensor platform. Free PVP-SE1 phages in solution showed the ability to recognize VBNC cells, with no lysis induction, in contrast to the minor recognition of heat-killed cells. This ability was confirmed for immobilized phages on gold surfaces, where the phage detection signal follows the same trend of the concentration of viable plus VBNC cells in the sample. The phage probe was then tested in a magnetoresistive biosensor platform allowing the quantitative detection and discrimination of viable and VBNC cells from dead cells, with high sensitivity. Signals arising from 3 to 4 cells per sensor were recorded. In comparison to a polyclonal antibody that does not distinguish viable from dead cells, the phage selectivity in cell recognition minimizes false-negative and false-positive results often associated with most detection methods.ElsevierUniversidade do MinhoFernandes, Elisabete RamosMartins, V. C.Nóbrega, CláudiaCarvalho, Carla A. O. C. M.Cardoso, F. A.Cardoso, S.Dias, J.Deng, D.Kluskens, LeonFreitas, P. P.Azeredo, Joana20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/27313eng0956-566310.1016/j.bios.2013.08.05324055938http://www.sciencedirect.com/science/article/pii/S0956566313006027info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:20:27Zoai:repositorium.sdum.uminho.pt:1822/27313Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:13:34.592937Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A bacteriophage detection tool for viability assessment of Salmonella cells
title A bacteriophage detection tool for viability assessment of Salmonella cells
spellingShingle A bacteriophage detection tool for viability assessment of Salmonella cells
Fernandes, Elisabete Ramos
Bacteriophage
Salmonella
Cell viability
Viable but non-culturable bacteria
Magnetoresistive biochip
Magnetic nanoparticles
Science & Technology
title_short A bacteriophage detection tool for viability assessment of Salmonella cells
title_full A bacteriophage detection tool for viability assessment of Salmonella cells
title_fullStr A bacteriophage detection tool for viability assessment of Salmonella cells
title_full_unstemmed A bacteriophage detection tool for viability assessment of Salmonella cells
title_sort A bacteriophage detection tool for viability assessment of Salmonella cells
author Fernandes, Elisabete Ramos
author_facet Fernandes, Elisabete Ramos
Martins, V. C.
Nóbrega, Cláudia
Carvalho, Carla A. O. C. M.
Cardoso, F. A.
Cardoso, S.
Dias, J.
Deng, D.
Kluskens, Leon
Freitas, P. P.
Azeredo, Joana
author_role author
author2 Martins, V. C.
Nóbrega, Cláudia
Carvalho, Carla A. O. C. M.
Cardoso, F. A.
Cardoso, S.
Dias, J.
Deng, D.
Kluskens, Leon
Freitas, P. P.
Azeredo, Joana
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Fernandes, Elisabete Ramos
Martins, V. C.
Nóbrega, Cláudia
Carvalho, Carla A. O. C. M.
Cardoso, F. A.
Cardoso, S.
Dias, J.
Deng, D.
Kluskens, Leon
Freitas, P. P.
Azeredo, Joana
dc.subject.por.fl_str_mv Bacteriophage
Salmonella
Cell viability
Viable but non-culturable bacteria
Magnetoresistive biochip
Magnetic nanoparticles
Science & Technology
topic Bacteriophage
Salmonella
Cell viability
Viable but non-culturable bacteria
Magnetoresistive biochip
Magnetic nanoparticles
Science & Technology
description Available online 7 September 2013
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/27313
url http://hdl.handle.net/1822/27313
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0956-5663
10.1016/j.bios.2013.08.053
24055938
http://www.sciencedirect.com/science/article/pii/S0956566313006027
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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