Antimicrobial and cytotoxic assessment of marine cyanobacteria - synechocystis and synechococcus
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/1720 |
Resumo: | Aqueous extracts and organic solvent extracts of isolated marine cyanobacteria strains were tested for antimicrobial activity against a fungus, Gram-positive and Gram-negative bacteria and for cytotoxic activity against primary rat hepatocytes and HL-60 cells. Antimicrobial activity was based on the agar diffusion assay. Cytotoxic activity was measured by apoptotic cell death scored by cell surface evaluation and nuclear morphology. A high percentage of apoptotic cells were observed for HL-60 cells when treated with cyanobacterial organic extracts. Slight apoptotic effects were observed in primary rat hepatocytes when exposed to aqueous cyanobacterial extracts. Nine cyanobacteria strains were found to have antibiotic activity against two Gram-positive bacteria, Clavibacter michiganensis subsp. insidiosum and Cellulomonas uda. No inhibitory effects were found against the fungus Candida albicans and Gram-negative bacteria. Marine Synechocystis and Synechococcus extracts induce apoptosis in eukaryotic cells and cause inhibition of Gram-positive bacteria. The different activity in different extracts suggests different compounds with different polarities. |
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Antimicrobial and cytotoxic assessment of marine cyanobacteria - synechocystis and synechococcusMarine cyanobacteriaSynechococcusAntibioticSynechocystisAqueous extracts and organic solvent extracts of isolated marine cyanobacteria strains were tested for antimicrobial activity against a fungus, Gram-positive and Gram-negative bacteria and for cytotoxic activity against primary rat hepatocytes and HL-60 cells. Antimicrobial activity was based on the agar diffusion assay. Cytotoxic activity was measured by apoptotic cell death scored by cell surface evaluation and nuclear morphology. A high percentage of apoptotic cells were observed for HL-60 cells when treated with cyanobacterial organic extracts. Slight apoptotic effects were observed in primary rat hepatocytes when exposed to aqueous cyanobacterial extracts. Nine cyanobacteria strains were found to have antibiotic activity against two Gram-positive bacteria, Clavibacter michiganensis subsp. insidiosum and Cellulomonas uda. No inhibitory effects were found against the fungus Candida albicans and Gram-negative bacteria. Marine Synechocystis and Synechococcus extracts induce apoptosis in eukaryotic cells and cause inhibition of Gram-positive bacteria. The different activity in different extracts suggests different compounds with different polarities.FCT - PRAXXIS XXI/BD/21758/99; POCI/CTA/46733/2002MDPIRepositório Científico do Instituto Politécnico do PortoMartins, RosarioRamos, MiguelHerfindal, LarsSousa, JoséSkærven, KajaVasconcelos, Vítor2013-06-19T13:13:12Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/1720engMartins, R. F., Ramos, M. F., Herfindal, L., Sousa, J. A., Skærven, K., & Vasconcelos, V. M. (2008). Antimicrobial and Cytotoxic Assessment of Marine Cyanobacteria—Synechocystis and Synechococcus. Marine Drugs, 6(1), Artigo 1. https://doi.org/10.3390/md60100011660-339710.3390/md6010001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-27T01:47:51Zoai:recipp.ipp.pt:10400.22/1720Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:22:49.944023Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antimicrobial and cytotoxic assessment of marine cyanobacteria - synechocystis and synechococcus |
title |
Antimicrobial and cytotoxic assessment of marine cyanobacteria - synechocystis and synechococcus |
spellingShingle |
Antimicrobial and cytotoxic assessment of marine cyanobacteria - synechocystis and synechococcus Martins, Rosario Marine cyanobacteria Synechococcus Antibiotic Synechocystis |
title_short |
Antimicrobial and cytotoxic assessment of marine cyanobacteria - synechocystis and synechococcus |
title_full |
Antimicrobial and cytotoxic assessment of marine cyanobacteria - synechocystis and synechococcus |
title_fullStr |
Antimicrobial and cytotoxic assessment of marine cyanobacteria - synechocystis and synechococcus |
title_full_unstemmed |
Antimicrobial and cytotoxic assessment of marine cyanobacteria - synechocystis and synechococcus |
title_sort |
Antimicrobial and cytotoxic assessment of marine cyanobacteria - synechocystis and synechococcus |
author |
Martins, Rosario |
author_facet |
Martins, Rosario Ramos, Miguel Herfindal, Lars Sousa, José Skærven, Kaja Vasconcelos, Vítor |
author_role |
author |
author2 |
Ramos, Miguel Herfindal, Lars Sousa, José Skærven, Kaja Vasconcelos, Vítor |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Martins, Rosario Ramos, Miguel Herfindal, Lars Sousa, José Skærven, Kaja Vasconcelos, Vítor |
dc.subject.por.fl_str_mv |
Marine cyanobacteria Synechococcus Antibiotic Synechocystis |
topic |
Marine cyanobacteria Synechococcus Antibiotic Synechocystis |
description |
Aqueous extracts and organic solvent extracts of isolated marine cyanobacteria strains were tested for antimicrobial activity against a fungus, Gram-positive and Gram-negative bacteria and for cytotoxic activity against primary rat hepatocytes and HL-60 cells. Antimicrobial activity was based on the agar diffusion assay. Cytotoxic activity was measured by apoptotic cell death scored by cell surface evaluation and nuclear morphology. A high percentage of apoptotic cells were observed for HL-60 cells when treated with cyanobacterial organic extracts. Slight apoptotic effects were observed in primary rat hepatocytes when exposed to aqueous cyanobacterial extracts. Nine cyanobacteria strains were found to have antibiotic activity against two Gram-positive bacteria, Clavibacter michiganensis subsp. insidiosum and Cellulomonas uda. No inhibitory effects were found against the fungus Candida albicans and Gram-negative bacteria. Marine Synechocystis and Synechococcus extracts induce apoptosis in eukaryotic cells and cause inhibition of Gram-positive bacteria. The different activity in different extracts suggests different compounds with different polarities. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2008-01-01T00:00:00Z 2013-06-19T13:13:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/1720 |
url |
http://hdl.handle.net/10400.22/1720 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Martins, R. F., Ramos, M. F., Herfindal, L., Sousa, J. A., Skærven, K., & Vasconcelos, V. M. (2008). Antimicrobial and Cytotoxic Assessment of Marine Cyanobacteria—Synechocystis and Synechococcus. Marine Drugs, 6(1), Artigo 1. https://doi.org/10.3390/md6010001 1660-3397 10.3390/md6010001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131324214149120 |