Indoleacetic acid production and chromium reduction by cyanobacteria Synechocystis SP. P2A (Chroococcales) immobilized in alginate beads

Detalhes bibliográficos
Autor(a) principal: Zahid, Chand
Data de Publicação: 2017
Outros Autores: Khurshid, Sana, Husnain, Shahida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/32731
Resumo: A simple extrusion method was used to entrap Synechocystis sp.P2A in alginate beads. The viability, growth response and Indoleacetic acid (IAA) production at different pH were studied in alginate immobilized Synechocystis sp.P2A. 2.6% sodium alginate (w/v) and pH-7 was found to be optimum for growth of Synechocystis sp. P2A as well as IAA production (79µg/ml). To prepare effective formulation for plant inoculation, alginate beads were further modified by coating with chitosan or chitosan-polyethylene glycol. Effect of all formulations containing Synechocystis sp. P2A in free and immobilized form on growth of Triticumaestivum was evaluated. Soil inoculation of entrapped Synechocystis in alginate beads coated with chitosan resulted in 20% increase in root length and 14% increase in dry weight as compared to non-inoculated seedlings. Free and immobilized cyanobacteria were allowed to grow in BG11 medium supplemented with 100μg/ml K2CrO4 and chromium reduction was measured at variable pH. At pH 7 immobilized showed 5% more reduction than free form. The current study showed that alginate immobilized Synechocystis sp. P2A can accomplish viable functions including plant growth promoting hormone production and chromium reduction and therefore propose an efficient and convenient method for storage and use of cyanobacteria.
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spelling Indoleacetic acid production and chromium reduction by cyanobacteria Synechocystis SP. P2A (Chroococcales) immobilized in alginate beads Produção de ácido indolacético e redução do cromo por cianobactérias Synechocystis SP. P2A (Chroococcales) imobilizadas em esferas de alginato ImmobilizationChitosanCyanobacteriaAuxinSynechocystisA simple extrusion method was used to entrap Synechocystis sp.P2A in alginate beads. The viability, growth response and Indoleacetic acid (IAA) production at different pH were studied in alginate immobilized Synechocystis sp.P2A. 2.6% sodium alginate (w/v) and pH-7 was found to be optimum for growth of Synechocystis sp. P2A as well as IAA production (79µg/ml). To prepare effective formulation for plant inoculation, alginate beads were further modified by coating with chitosan or chitosan-polyethylene glycol. Effect of all formulations containing Synechocystis sp. P2A in free and immobilized form on growth of Triticumaestivum was evaluated. Soil inoculation of entrapped Synechocystis in alginate beads coated with chitosan resulted in 20% increase in root length and 14% increase in dry weight as compared to non-inoculated seedlings. Free and immobilized cyanobacteria were allowed to grow in BG11 medium supplemented with 100μg/ml K2CrO4 and chromium reduction was measured at variable pH. At pH 7 immobilized showed 5% more reduction than free form. The current study showed that alginate immobilized Synechocystis sp. P2A can accomplish viable functions including plant growth promoting hormone production and chromium reduction and therefore propose an efficient and convenient method for storage and use of cyanobacteria.Um método de extrusão simples foi utilizado para aprisionar Synechocystis sp.P2A em esferas de alginato. A viabilidade, a resposta ao crescimento e a produção de ácido indolacético (IAA) a diferentes pH foram estudadas na Synechocystis sp.P2A imobilizada com alginato. Alginato de sódio a 2,6% (p/v) e pH-7 revelou-se ótimo para o crescimento de Synechocystis sp. P2A, bem como para a produção de IAA (79 µg/ml). Para preparar uma formulação eficaz para inoculação de plantas, as esferas de alginato foram adicionalmente modificadas por revestimento com quitosano ou quitosano-polietileno glicol. O efeito de todas as formulações contendo Synechocystis sp. P2A em forma livre e imobilizada no crescimento de Triticumaestivum foi avaliado. A inoculação no solo com Synechocystis aprisionado em esferas de alginato revestidas com quitosano resultou em um aumento de 20% no comprimento da raiz e aumento de 14% no peso seco em comparação com mudas não inoculadas. As cianobactérias livres e imobilizadas foram deixadas crescer em meio BG11 suplementado com 100 µg/ml de K2CrO4 e a redução do cromo foi medida a um pH variável. A um pH 7 a forma imobilizada apresentou 5% mais de redução do que a forma livre. O presente estudo mostrou que o alginato imobilizado de Synechocystis sp. P2A pode realizar funções viáveis, incluindo a produção de hormônio promotor do crescimento de plantas e redução de cromo e, portanto, propor um método eficiente e conveniente para armazenamento e uso de cianobactérias.EDUFU2017-11-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/3273110.14393/BJ-v33n6a2017-32731Bioscience Journal ; Vol. 33 No. 6 (2017): Nov./Dec.; 1592-1600Bioscience Journal ; v. 33 n. 6 (2017): Nov./Dec.; 1592-16001981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/32731/21225Brazil; ContemporaryCopyright (c) 2017 Sana Khurshid, Chand Zahid, Shahida Husnainhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessZahid, ChandKhurshid, SanaHusnain, Shahida2022-02-12T01:15:50Zoai:ojs.www.seer.ufu.br:article/32731Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-02-12T01:15:50Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Indoleacetic acid production and chromium reduction by cyanobacteria Synechocystis SP. P2A (Chroococcales) immobilized in alginate beads
Produção de ácido indolacético e redução do cromo por cianobactérias Synechocystis SP. P2A (Chroococcales) imobilizadas em esferas de alginato
title Indoleacetic acid production and chromium reduction by cyanobacteria Synechocystis SP. P2A (Chroococcales) immobilized in alginate beads
spellingShingle Indoleacetic acid production and chromium reduction by cyanobacteria Synechocystis SP. P2A (Chroococcales) immobilized in alginate beads
Zahid, Chand
Immobilization
Chitosan
Cyanobacteria
Auxin
Synechocystis
title_short Indoleacetic acid production and chromium reduction by cyanobacteria Synechocystis SP. P2A (Chroococcales) immobilized in alginate beads
title_full Indoleacetic acid production and chromium reduction by cyanobacteria Synechocystis SP. P2A (Chroococcales) immobilized in alginate beads
title_fullStr Indoleacetic acid production and chromium reduction by cyanobacteria Synechocystis SP. P2A (Chroococcales) immobilized in alginate beads
title_full_unstemmed Indoleacetic acid production and chromium reduction by cyanobacteria Synechocystis SP. P2A (Chroococcales) immobilized in alginate beads
title_sort Indoleacetic acid production and chromium reduction by cyanobacteria Synechocystis SP. P2A (Chroococcales) immobilized in alginate beads
author Zahid, Chand
author_facet Zahid, Chand
Khurshid, Sana
Husnain, Shahida
author_role author
author2 Khurshid, Sana
Husnain, Shahida
author2_role author
author
dc.contributor.author.fl_str_mv Zahid, Chand
Khurshid, Sana
Husnain, Shahida
dc.subject.por.fl_str_mv Immobilization
Chitosan
Cyanobacteria
Auxin
Synechocystis
topic Immobilization
Chitosan
Cyanobacteria
Auxin
Synechocystis
description A simple extrusion method was used to entrap Synechocystis sp.P2A in alginate beads. The viability, growth response and Indoleacetic acid (IAA) production at different pH were studied in alginate immobilized Synechocystis sp.P2A. 2.6% sodium alginate (w/v) and pH-7 was found to be optimum for growth of Synechocystis sp. P2A as well as IAA production (79µg/ml). To prepare effective formulation for plant inoculation, alginate beads were further modified by coating with chitosan or chitosan-polyethylene glycol. Effect of all formulations containing Synechocystis sp. P2A in free and immobilized form on growth of Triticumaestivum was evaluated. Soil inoculation of entrapped Synechocystis in alginate beads coated with chitosan resulted in 20% increase in root length and 14% increase in dry weight as compared to non-inoculated seedlings. Free and immobilized cyanobacteria were allowed to grow in BG11 medium supplemented with 100μg/ml K2CrO4 and chromium reduction was measured at variable pH. At pH 7 immobilized showed 5% more reduction than free form. The current study showed that alginate immobilized Synechocystis sp. P2A can accomplish viable functions including plant growth promoting hormone production and chromium reduction and therefore propose an efficient and convenient method for storage and use of cyanobacteria.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/32731
10.14393/BJ-v33n6a2017-32731
url https://seer.ufu.br/index.php/biosciencejournal/article/view/32731
identifier_str_mv 10.14393/BJ-v33n6a2017-32731
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/32731/21225
dc.rights.driver.fl_str_mv Copyright (c) 2017 Sana Khurshid, Chand Zahid, Shahida Husnain
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Sana Khurshid, Chand Zahid, Shahida Husnain
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporary
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 33 No. 6 (2017): Nov./Dec.; 1592-1600
Bioscience Journal ; v. 33 n. 6 (2017): Nov./Dec.; 1592-1600
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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