Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan

Detalhes bibliográficos
Autor(a) principal: Campos, Débora A.
Data de Publicação: 2017
Outros Autores: Valetti, Nadia Woitovich, Oliveira, Ana, Pastrana-Castro, Lorenzo M., Teixeira, J. A., Pintado, Maria Manuela, Guillermo Picó
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/46337
Resumo: The main objective of this work was to investigate for the first time the molecular mechanism of complex formation between bromelain (a positively charged enzyme) and carrageenan (a natural strong polyelectrolyte, negatively charged) using spectroscopy techniques and thermodynamic approaches. The Bromelain-Carrageenan complex showed a maximal non-solubility at pH around 5.1. The solubility was dependent on pH and ionic strength of the medium. To re-dissolve the formed complex, the pH was changed and 500 mM of NaCl was added to the initial solution, proving the columbic mechanism for the formation of non-soluble complex. The formation of the carrageenan-bromelain complex increased in 8 °C the enzyme thermal stability, while its biological activity was not modified. The amount of total enzyme recovered in solution after precipitation with around 0.08% w/v of carrageenan was 8590%.
id RCAP_6dbf0d226129aec3d0aacc13f8f5b86d
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/46337
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenanProtein complexEnzyme mechanismCarrageenanPolyelectrolytesBromelainScience & TechnologyThe main objective of this work was to investigate for the first time the molecular mechanism of complex formation between bromelain (a positively charged enzyme) and carrageenan (a natural strong polyelectrolyte, negatively charged) using spectroscopy techniques and thermodynamic approaches. The Bromelain-Carrageenan complex showed a maximal non-solubility at pH around 5.1. The solubility was dependent on pH and ionic strength of the medium. To re-dissolve the formed complex, the pH was changed and 500 mM of NaCl was added to the initial solution, proving the columbic mechanism for the formation of non-soluble complex. The formation of the carrageenan-bromelain complex increased in 8 °C the enzyme thermal stability, while its biological activity was not modified. The amount of total enzyme recovered in solution after precipitation with around 0.08% w/v of carrageenan was 8590%.The work was financially supported by BiValBi − Biotechnologies to Valorise the regional Biodiversity in Latin America, FCT − Fundação para a Ciência e a Tecnologia through project UID/Multi/50016/2013 and by PhD grant SFRH/BD/104074/2014 to Débora Campos.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoCampos, Débora A.Valetti, Nadia WoitovichOliveira, AnaPastrana-Castro, Lorenzo M.Teixeira, J. A.Pintado, Maria ManuelaGuillermo Picó2017-032017-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/46337engCampos, Débora A.; Valetti, Nadia Woitovich; Oliveira, Ana; Pastrana-Castro, Lorenzo M.; Teixeira, José A.; Pintado, Maria Manuela; Guillermo Picó, Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan. Process Biochemistry, 54, 156-161, 20171359-511310.1016/j.procbio.2016.12.014http://www.journals.elsevier.com/process-biochemistry/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:03:18Zoai:repositorium.sdum.uminho.pt:1822/46337Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:53:24.901740Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan
title Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan
spellingShingle Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan
Campos, Débora A.
Protein complex
Enzyme mechanism
Carrageenan
Polyelectrolytes
Bromelain
Science & Technology
title_short Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan
title_full Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan
title_fullStr Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan
title_full_unstemmed Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan
title_sort Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan
author Campos, Débora A.
author_facet Campos, Débora A.
Valetti, Nadia Woitovich
Oliveira, Ana
Pastrana-Castro, Lorenzo M.
Teixeira, J. A.
Pintado, Maria Manuela
Guillermo Picó
author_role author
author2 Valetti, Nadia Woitovich
Oliveira, Ana
Pastrana-Castro, Lorenzo M.
Teixeira, J. A.
Pintado, Maria Manuela
Guillermo Picó
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Campos, Débora A.
Valetti, Nadia Woitovich
Oliveira, Ana
Pastrana-Castro, Lorenzo M.
Teixeira, J. A.
Pintado, Maria Manuela
Guillermo Picó
dc.subject.por.fl_str_mv Protein complex
Enzyme mechanism
Carrageenan
Polyelectrolytes
Bromelain
Science & Technology
topic Protein complex
Enzyme mechanism
Carrageenan
Polyelectrolytes
Bromelain
Science & Technology
description The main objective of this work was to investigate for the first time the molecular mechanism of complex formation between bromelain (a positively charged enzyme) and carrageenan (a natural strong polyelectrolyte, negatively charged) using spectroscopy techniques and thermodynamic approaches. The Bromelain-Carrageenan complex showed a maximal non-solubility at pH around 5.1. The solubility was dependent on pH and ionic strength of the medium. To re-dissolve the formed complex, the pH was changed and 500 mM of NaCl was added to the initial solution, proving the columbic mechanism for the formation of non-soluble complex. The formation of the carrageenan-bromelain complex increased in 8 °C the enzyme thermal stability, while its biological activity was not modified. The amount of total enzyme recovered in solution after precipitation with around 0.08% w/v of carrageenan was 8590%.
publishDate 2017
dc.date.none.fl_str_mv 2017-03
2017-03-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/46337
url http://hdl.handle.net/1822/46337
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Campos, Débora A.; Valetti, Nadia Woitovich; Oliveira, Ana; Pastrana-Castro, Lorenzo M.; Teixeira, José A.; Pintado, Maria Manuela; Guillermo Picó, Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan. Process Biochemistry, 54, 156-161, 2017
1359-5113
10.1016/j.procbio.2016.12.014
http://www.journals.elsevier.com/process-biochemistry/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132314069893120

Registros relacionados