Physicochemical and biological evaluation of poly(ethylene glycol) methacrylate grafted onto poly(dimethyl siloxane) surfaces for prosthetic devices

Detalhes bibliográficos
Autor(a) principal: Gonçalves, S.
Data de Publicação: 2013
Outros Autores: Leirós, Ana Catarina Correia, Van Kooten, Theo, Dourado, Fernando, Rodrigues, L. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/24959
Resumo: Poly(dimethyl siloxane) (PDMS) was surface-polymerized with poly(ethylene glycol)methacrylate (PEGMA) by surface-initiated atom transfer radical polymerization (SI-ATRP) in aqueous media at room temperature. Modification of the PDMS surface followed a three-step procedure: (i) PDMS surface hydroxylation by UV/ozone exposure, immediately followed by (ii) covalent attachment of the initiator, 1-trichlorosilyl-2-(chloromethylphenyl)ethane, onto the hydroxylated PDMS, via chemical vapor deposition; finally (iii) PDMS surface-polymerization of PEGMA by ATRP. Modified PDMS was characterized by water contact angle measurement, SEM, FTIR-ATR, and XPS. Results showed that modified surfaces had a hydrophilic character, given the water contact angles around 60◦; FTIR-ATR and XPS analysis confirmed the presence of polymerized PEGMA on the surface of PDMS and the adhesion of Staphylococcus aureus GB 2/1 and Streptococcus salivarius GB 24/9 onto the modified surfaces was inhibited 94% and 81%, respectively. Finally, the modified PDMS showed no evidence of cytotoxic effects in in vitro assays using human skin fibroblasts.
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spelling Physicochemical and biological evaluation of poly(ethylene glycol) methacrylate grafted onto poly(dimethyl siloxane) surfaces for prosthetic devicesPoly(dimethyl siloxane)Surface-initiated atom transfer radicalPolymerizationAnti-adhesionCytotoxicitySurface-initiated atom transfer radical polymerizationScience & TechnologyPoly(dimethyl siloxane) (PDMS) was surface-polymerized with poly(ethylene glycol)methacrylate (PEGMA) by surface-initiated atom transfer radical polymerization (SI-ATRP) in aqueous media at room temperature. Modification of the PDMS surface followed a three-step procedure: (i) PDMS surface hydroxylation by UV/ozone exposure, immediately followed by (ii) covalent attachment of the initiator, 1-trichlorosilyl-2-(chloromethylphenyl)ethane, onto the hydroxylated PDMS, via chemical vapor deposition; finally (iii) PDMS surface-polymerization of PEGMA by ATRP. Modified PDMS was characterized by water contact angle measurement, SEM, FTIR-ATR, and XPS. Results showed that modified surfaces had a hydrophilic character, given the water contact angles around 60◦; FTIR-ATR and XPS analysis confirmed the presence of polymerized PEGMA on the surface of PDMS and the adhesion of Staphylococcus aureus GB 2/1 and Streptococcus salivarius GB 24/9 onto the modified surfaces was inhibited 94% and 81%, respectively. Finally, the modified PDMS showed no evidence of cytotoxic effects in in vitro assays using human skin fibroblasts.The authors acknowledge Stockwell Elastomerics Inc. for kindly proving the silicone rubber samples (ref. HT6240). SEM, FTIR-ATR and XPS studies were performed at SEMAT (University of Minho, Portugal), DEP (University of Minho, Portugal) and CEMUP (University of Porto, Portugal) facilities, respectively. Also, the authors acknowledge the financial support from Fundacao para a Ciencia e Tecnologia (FCT) to the project BIOSURFA - Application of biosurfactants for microbial adhesion inhibition in medical devices (PTDC/SAU-BEB/73498/2006).Elsevier Science BVElsevier BVUniversidade do MinhoGonçalves, S.Leirós, Ana Catarina CorreiaVan Kooten, TheoDourado, FernandoRodrigues, L. R.20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/24959eng0927-77650927-776510.1016/j.colsurfb.2013.03.05023660308info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:02Zoai:repositorium.sdum.uminho.pt:1822/24959Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:12.736376Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Physicochemical and biological evaluation of poly(ethylene glycol) methacrylate grafted onto poly(dimethyl siloxane) surfaces for prosthetic devices
title Physicochemical and biological evaluation of poly(ethylene glycol) methacrylate grafted onto poly(dimethyl siloxane) surfaces for prosthetic devices
spellingShingle Physicochemical and biological evaluation of poly(ethylene glycol) methacrylate grafted onto poly(dimethyl siloxane) surfaces for prosthetic devices
Gonçalves, S.
Poly(dimethyl siloxane)
Surface-initiated atom transfer radical
Polymerization
Anti-adhesion
Cytotoxicity
Surface-initiated atom transfer radical polymerization
Science & Technology
title_short Physicochemical and biological evaluation of poly(ethylene glycol) methacrylate grafted onto poly(dimethyl siloxane) surfaces for prosthetic devices
title_full Physicochemical and biological evaluation of poly(ethylene glycol) methacrylate grafted onto poly(dimethyl siloxane) surfaces for prosthetic devices
title_fullStr Physicochemical and biological evaluation of poly(ethylene glycol) methacrylate grafted onto poly(dimethyl siloxane) surfaces for prosthetic devices
title_full_unstemmed Physicochemical and biological evaluation of poly(ethylene glycol) methacrylate grafted onto poly(dimethyl siloxane) surfaces for prosthetic devices
title_sort Physicochemical and biological evaluation of poly(ethylene glycol) methacrylate grafted onto poly(dimethyl siloxane) surfaces for prosthetic devices
author Gonçalves, S.
author_facet Gonçalves, S.
Leirós, Ana Catarina Correia
Van Kooten, Theo
Dourado, Fernando
Rodrigues, L. R.
author_role author
author2 Leirós, Ana Catarina Correia
Van Kooten, Theo
Dourado, Fernando
Rodrigues, L. R.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gonçalves, S.
Leirós, Ana Catarina Correia
Van Kooten, Theo
Dourado, Fernando
Rodrigues, L. R.
dc.subject.por.fl_str_mv Poly(dimethyl siloxane)
Surface-initiated atom transfer radical
Polymerization
Anti-adhesion
Cytotoxicity
Surface-initiated atom transfer radical polymerization
Science & Technology
topic Poly(dimethyl siloxane)
Surface-initiated atom transfer radical
Polymerization
Anti-adhesion
Cytotoxicity
Surface-initiated atom transfer radical polymerization
Science & Technology
description Poly(dimethyl siloxane) (PDMS) was surface-polymerized with poly(ethylene glycol)methacrylate (PEGMA) by surface-initiated atom transfer radical polymerization (SI-ATRP) in aqueous media at room temperature. Modification of the PDMS surface followed a three-step procedure: (i) PDMS surface hydroxylation by UV/ozone exposure, immediately followed by (ii) covalent attachment of the initiator, 1-trichlorosilyl-2-(chloromethylphenyl)ethane, onto the hydroxylated PDMS, via chemical vapor deposition; finally (iii) PDMS surface-polymerization of PEGMA by ATRP. Modified PDMS was characterized by water contact angle measurement, SEM, FTIR-ATR, and XPS. Results showed that modified surfaces had a hydrophilic character, given the water contact angles around 60◦; FTIR-ATR and XPS analysis confirmed the presence of polymerized PEGMA on the surface of PDMS and the adhesion of Staphylococcus aureus GB 2/1 and Streptococcus salivarius GB 24/9 onto the modified surfaces was inhibited 94% and 81%, respectively. Finally, the modified PDMS showed no evidence of cytotoxic effects in in vitro assays using human skin fibroblasts.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/24959
url http://hdl.handle.net/1822/24959
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0927-7765
0927-7765
10.1016/j.colsurfb.2013.03.050
23660308
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Science BV
Elsevier BV
publisher.none.fl_str_mv Elsevier Science BV
Elsevier BV
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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