A two-component pre-seeded dermal-epidermal scaffold

Detalhes bibliográficos
Autor(a) principal: Monteiro, I. P.
Data de Publicação: 2014
Outros Autores: Gabriel, D., Timko, B. P., Hashimoto, M., Karajanagi, S., Tong, R., Marques, A. P., Reis, R. L., Kohane, D. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/44457
Resumo: We have developed a bilayered dermal-epidermal scaffold for application in the treatment of full-thickness skin defects. The dermal component gels in situ and adapts to the lesion shape, delivering human dermal fibroblasts in a matrix of fibrin and cross-linked hyaluronic acid modified with a cell adhesion-promoting peptide. Fibroblasts were able to form a tridimensional matrix due to material features such as tailored mechanical properties, presence of protease-degradable elements and cell-binding ligands. The epidermal component is a robust membrane containing cross-linked hyaluronic acid and poly-l-lysine, on which keratinocytes were able to attach and to form a monolayer. Amine-aldehyde bonding at the interface between the two components allows the formation of a tightly bound composite scaffold. Both parts of the scaffold were designed to provide cell-type-specific cues to allow for cell proliferation and form a construct that mimics the skin environment.
id RCAP_6df8b2d087eb099d6fee5e582fc72639
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/44457
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling A two-component pre-seeded dermal-epidermal scaffoldSkin defectsBilayeredHyaluronic acidAmine–aldehyde bondingScience & TechnologyWe have developed a bilayered dermal-epidermal scaffold for application in the treatment of full-thickness skin defects. The dermal component gels in situ and adapts to the lesion shape, delivering human dermal fibroblasts in a matrix of fibrin and cross-linked hyaluronic acid modified with a cell adhesion-promoting peptide. Fibroblasts were able to form a tridimensional matrix due to material features such as tailored mechanical properties, presence of protease-degradable elements and cell-binding ligands. The epidermal component is a robust membrane containing cross-linked hyaluronic acid and poly-l-lysine, on which keratinocytes were able to attach and to form a monolayer. Amine-aldehyde bonding at the interface between the two components allows the formation of a tightly bound composite scaffold. Both parts of the scaffold were designed to provide cell-type-specific cues to allow for cell proliferation and form a construct that mimics the skin environment.D.S.K. acknowledges funding from the Biotechnology Research Endowment from the Department of Anesthesiology at Boston Children's Hospital. I.P.M. acknowledges the Portuguese Foundation for Science and Technology for the grant BD/39396/2007 and the MIT-Portugal Program. D.G. acknowledges the Swiss National Science Foundation for a post-doctoral fellowship (PBGEP3-129111). B.P.T. acknowledges an NIR Ruth L. Kirschstein National Research Service Award (F32GM096546).ElsevierUniversidade do MinhoMonteiro, I. P.Gabriel, D.Timko, B. P.Hashimoto, M.Karajanagi, S.Tong, R.Marques, A. P.Reis, R. L.Kohane, D. S.2014-092014-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/44457engMonteiro I. P., Gabriel D., Timko B. P., Hashimoto M., Karajanagi S., Tong R., Marques A. P., Reis R. L., Kohane D. S. A two-component pre-seeded dermal-epidermal scaffold, Acta Biomaterialia, Vol. 10, Issue 12, pp. 4928-4938, doi:10.1016/j.actbio.2014.08.029, 20141742-706110.1016/j.actbio.2014.08.02925192821http://www.sciencedirect.com/science/article/pii/S1742706114003742info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T04:24:35Zoai:repositorium.sdum.uminho.pt:1822/44457Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T04:24:35Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A two-component pre-seeded dermal-epidermal scaffold
title A two-component pre-seeded dermal-epidermal scaffold
spellingShingle A two-component pre-seeded dermal-epidermal scaffold
Monteiro, I. P.
Skin defects
Bilayered
Hyaluronic acid
Amine–aldehyde bonding
Science & Technology
title_short A two-component pre-seeded dermal-epidermal scaffold
title_full A two-component pre-seeded dermal-epidermal scaffold
title_fullStr A two-component pre-seeded dermal-epidermal scaffold
title_full_unstemmed A two-component pre-seeded dermal-epidermal scaffold
title_sort A two-component pre-seeded dermal-epidermal scaffold
author Monteiro, I. P.
author_facet Monteiro, I. P.
Gabriel, D.
Timko, B. P.
Hashimoto, M.
Karajanagi, S.
Tong, R.
Marques, A. P.
Reis, R. L.
Kohane, D. S.
author_role author
author2 Gabriel, D.
Timko, B. P.
Hashimoto, M.
Karajanagi, S.
Tong, R.
Marques, A. P.
Reis, R. L.
Kohane, D. S.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Monteiro, I. P.
Gabriel, D.
Timko, B. P.
Hashimoto, M.
Karajanagi, S.
Tong, R.
Marques, A. P.
Reis, R. L.
Kohane, D. S.
dc.subject.por.fl_str_mv Skin defects
Bilayered
Hyaluronic acid
Amine–aldehyde bonding
Science & Technology
topic Skin defects
Bilayered
Hyaluronic acid
Amine–aldehyde bonding
Science & Technology
description We have developed a bilayered dermal-epidermal scaffold for application in the treatment of full-thickness skin defects. The dermal component gels in situ and adapts to the lesion shape, delivering human dermal fibroblasts in a matrix of fibrin and cross-linked hyaluronic acid modified with a cell adhesion-promoting peptide. Fibroblasts were able to form a tridimensional matrix due to material features such as tailored mechanical properties, presence of protease-degradable elements and cell-binding ligands. The epidermal component is a robust membrane containing cross-linked hyaluronic acid and poly-l-lysine, on which keratinocytes were able to attach and to form a monolayer. Amine-aldehyde bonding at the interface between the two components allows the formation of a tightly bound composite scaffold. Both parts of the scaffold were designed to provide cell-type-specific cues to allow for cell proliferation and form a construct that mimics the skin environment.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
2014-09-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/44457
url http://hdl.handle.net/1822/44457
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Monteiro I. P., Gabriel D., Timko B. P., Hashimoto M., Karajanagi S., Tong R., Marques A. P., Reis R. L., Kohane D. S. A two-component pre-seeded dermal-epidermal scaffold, Acta Biomaterialia, Vol. 10, Issue 12, pp. 4928-4938, doi:10.1016/j.actbio.2014.08.029, 2014
1742-7061
10.1016/j.actbio.2014.08.029
25192821
http://www.sciencedirect.com/science/article/pii/S1742706114003742
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817544301243006976