A two-component pre-seeded dermal-epidermal scaffold
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/44457 |
Resumo: | We have developed a bilayered dermal-epidermal scaffold for application in the treatment of full-thickness skin defects. The dermal component gels in situ and adapts to the lesion shape, delivering human dermal fibroblasts in a matrix of fibrin and cross-linked hyaluronic acid modified with a cell adhesion-promoting peptide. Fibroblasts were able to form a tridimensional matrix due to material features such as tailored mechanical properties, presence of protease-degradable elements and cell-binding ligands. The epidermal component is a robust membrane containing cross-linked hyaluronic acid and poly-l-lysine, on which keratinocytes were able to attach and to form a monolayer. Amine-aldehyde bonding at the interface between the two components allows the formation of a tightly bound composite scaffold. Both parts of the scaffold were designed to provide cell-type-specific cues to allow for cell proliferation and form a construct that mimics the skin environment. |
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A two-component pre-seeded dermal-epidermal scaffoldSkin defectsBilayeredHyaluronic acidAmine–aldehyde bondingScience & TechnologyWe have developed a bilayered dermal-epidermal scaffold for application in the treatment of full-thickness skin defects. The dermal component gels in situ and adapts to the lesion shape, delivering human dermal fibroblasts in a matrix of fibrin and cross-linked hyaluronic acid modified with a cell adhesion-promoting peptide. Fibroblasts were able to form a tridimensional matrix due to material features such as tailored mechanical properties, presence of protease-degradable elements and cell-binding ligands. The epidermal component is a robust membrane containing cross-linked hyaluronic acid and poly-l-lysine, on which keratinocytes were able to attach and to form a monolayer. Amine-aldehyde bonding at the interface between the two components allows the formation of a tightly bound composite scaffold. Both parts of the scaffold were designed to provide cell-type-specific cues to allow for cell proliferation and form a construct that mimics the skin environment.D.S.K. acknowledges funding from the Biotechnology Research Endowment from the Department of Anesthesiology at Boston Children's Hospital. I.P.M. acknowledges the Portuguese Foundation for Science and Technology for the grant BD/39396/2007 and the MIT-Portugal Program. D.G. acknowledges the Swiss National Science Foundation for a post-doctoral fellowship (PBGEP3-129111). B.P.T. acknowledges an NIR Ruth L. Kirschstein National Research Service Award (F32GM096546).ElsevierUniversidade do MinhoMonteiro, I. P.Gabriel, D.Timko, B. P.Hashimoto, M.Karajanagi, S.Tong, R.Marques, A. P.Reis, R. L.Kohane, D. S.2014-092014-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/44457engMonteiro I. P., Gabriel D., Timko B. P., Hashimoto M., Karajanagi S., Tong R., Marques A. P., Reis R. L., Kohane D. S. A two-component pre-seeded dermal-epidermal scaffold, Acta Biomaterialia, Vol. 10, Issue 12, pp. 4928-4938, doi:10.1016/j.actbio.2014.08.029, 20141742-706110.1016/j.actbio.2014.08.02925192821http://www.sciencedirect.com/science/article/pii/S1742706114003742info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T04:24:35Zoai:repositorium.sdum.uminho.pt:1822/44457Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T04:24:35Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A two-component pre-seeded dermal-epidermal scaffold |
title |
A two-component pre-seeded dermal-epidermal scaffold |
spellingShingle |
A two-component pre-seeded dermal-epidermal scaffold Monteiro, I. P. Skin defects Bilayered Hyaluronic acid Amine–aldehyde bonding Science & Technology |
title_short |
A two-component pre-seeded dermal-epidermal scaffold |
title_full |
A two-component pre-seeded dermal-epidermal scaffold |
title_fullStr |
A two-component pre-seeded dermal-epidermal scaffold |
title_full_unstemmed |
A two-component pre-seeded dermal-epidermal scaffold |
title_sort |
A two-component pre-seeded dermal-epidermal scaffold |
author |
Monteiro, I. P. |
author_facet |
Monteiro, I. P. Gabriel, D. Timko, B. P. Hashimoto, M. Karajanagi, S. Tong, R. Marques, A. P. Reis, R. L. Kohane, D. S. |
author_role |
author |
author2 |
Gabriel, D. Timko, B. P. Hashimoto, M. Karajanagi, S. Tong, R. Marques, A. P. Reis, R. L. Kohane, D. S. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Monteiro, I. P. Gabriel, D. Timko, B. P. Hashimoto, M. Karajanagi, S. Tong, R. Marques, A. P. Reis, R. L. Kohane, D. S. |
dc.subject.por.fl_str_mv |
Skin defects Bilayered Hyaluronic acid Amine–aldehyde bonding Science & Technology |
topic |
Skin defects Bilayered Hyaluronic acid Amine–aldehyde bonding Science & Technology |
description |
We have developed a bilayered dermal-epidermal scaffold for application in the treatment of full-thickness skin defects. The dermal component gels in situ and adapts to the lesion shape, delivering human dermal fibroblasts in a matrix of fibrin and cross-linked hyaluronic acid modified with a cell adhesion-promoting peptide. Fibroblasts were able to form a tridimensional matrix due to material features such as tailored mechanical properties, presence of protease-degradable elements and cell-binding ligands. The epidermal component is a robust membrane containing cross-linked hyaluronic acid and poly-l-lysine, on which keratinocytes were able to attach and to form a monolayer. Amine-aldehyde bonding at the interface between the two components allows the formation of a tightly bound composite scaffold. Both parts of the scaffold were designed to provide cell-type-specific cues to allow for cell proliferation and form a construct that mimics the skin environment. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09 2014-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/44457 |
url |
http://hdl.handle.net/1822/44457 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Monteiro I. P., Gabriel D., Timko B. P., Hashimoto M., Karajanagi S., Tong R., Marques A. P., Reis R. L., Kohane D. S. A two-component pre-seeded dermal-epidermal scaffold, Acta Biomaterialia, Vol. 10, Issue 12, pp. 4928-4938, doi:10.1016/j.actbio.2014.08.029, 2014 1742-7061 10.1016/j.actbio.2014.08.029 25192821 http://www.sciencedirect.com/science/article/pii/S1742706114003742 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817544301243006976 |