A phenotypic screening bioassay for Escherichia coli stress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysis

Detalhes bibliográficos
Autor(a) principal: Ribeiro Da Cunha, Bernardo
Data de Publicação: 2019
Outros Autores: Fonseca, Luís P. P., Calado, Cecília
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/10747
Resumo: Este trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2017 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISEL
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spelling A phenotypic screening bioassay for Escherichia coli stress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysisAntibioticsEscherichia coliFourier-transform infrared spectroscopyMetabolismOptimizationStress responseEste trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2017 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISELEste trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2018 do Instituto Politécnico de Lisboa. Código de referência IPL/2018/RENALPROG_ISELAims: To develop and optimize a Fourier-transform infrared spectroscopy (FTIRS) phenotypic screening bioassay for stress responses, regarding the effect of nutrient content, bacterial growth phase and stress agent exposure time. Methods and Results: A high-throughput FTIRS bioassay was developed to distinguish the stress responses of Escherichia coli to sodium hydroxide, hydrochloric acid, sodium chloride, sodium hypochlorite and ethanol. Principal component analysis and hierarchical clustering were used to quantify the effect of each parameter on bioassay performance, namely its reproducibility and metabolic resolution. Bioassay performance varied greatly, ranging from poor to very good. Spectra were partitioned into biologically relevant regions to evaluate their contributions to bioassay performance, but further improvements were not observed. Bioassay optimization was validated against empirical parameters, which confirmed a closer representation of known mechanisms on the antibiotic-induced stress responses. Conclusions: The optimized bioassay used standard nutrient content, cells in the late-stationary growth phase and a one-shift exposure duration. Only the optimized bioassay adequately and reproducibly distinguished the E. coli stress and antibiotic responses. The absence of performance improvements using partitioned spectra indicated that stress responses are imprinted on the wholespectra metabolic signature. Significance and Impact of the Study: Highly optimized FTIRS bioassay parameters are vital in capturing whole-spectra metabolic signatures that can be used for satisfactory and reproducible phenotypic screening of stress and antibiotic responses.The Society for Applied MicrobiologyRCIPLRibeiro Da Cunha, BernardoFonseca, Luís P. P.Calado, Cecília2019-11-26T14:29:24Z2019-122019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/10747engCUNHA, Bernardo Ribeiro; FONSECA, Luís P.; CALADO, Cecília R. C. – A phenotypic screening bioassay for Escherichia coli stress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysis. Journal of Applied Microbiology. ISSN 1365-2672. Vol. 127, N.º 6 (2019), pp. 1776-17891364-507210.1111/jam.14429metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:01:08Zoai:repositorio.ipl.pt:10400.21/10747Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:19:06.177850Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A phenotypic screening bioassay for Escherichia coli stress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysis
title A phenotypic screening bioassay for Escherichia coli stress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysis
spellingShingle A phenotypic screening bioassay for Escherichia coli stress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysis
Ribeiro Da Cunha, Bernardo
Antibiotics
Escherichia coli
Fourier-transform infrared spectroscopy
Metabolism
Optimization
Stress response
title_short A phenotypic screening bioassay for Escherichia coli stress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysis
title_full A phenotypic screening bioassay for Escherichia coli stress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysis
title_fullStr A phenotypic screening bioassay for Escherichia coli stress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysis
title_full_unstemmed A phenotypic screening bioassay for Escherichia coli stress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysis
title_sort A phenotypic screening bioassay for Escherichia coli stress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysis
author Ribeiro Da Cunha, Bernardo
author_facet Ribeiro Da Cunha, Bernardo
Fonseca, Luís P. P.
Calado, Cecília
author_role author
author2 Fonseca, Luís P. P.
Calado, Cecília
author2_role author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Ribeiro Da Cunha, Bernardo
Fonseca, Luís P. P.
Calado, Cecília
dc.subject.por.fl_str_mv Antibiotics
Escherichia coli
Fourier-transform infrared spectroscopy
Metabolism
Optimization
Stress response
topic Antibiotics
Escherichia coli
Fourier-transform infrared spectroscopy
Metabolism
Optimization
Stress response
description Este trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2017 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISEL
publishDate 2019
dc.date.none.fl_str_mv 2019-11-26T14:29:24Z
2019-12
2019-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/10747
url http://hdl.handle.net/10400.21/10747
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv CUNHA, Bernardo Ribeiro; FONSECA, Luís P.; CALADO, Cecília R. C. – A phenotypic screening bioassay for Escherichia coli stress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysis. Journal of Applied Microbiology. ISSN 1365-2672. Vol. 127, N.º 6 (2019), pp. 1776-1789
1364-5072
10.1111/jam.14429
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Society for Applied Microbiology
publisher.none.fl_str_mv The Society for Applied Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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